Antioxidant anthocyanins screening through spectrum–effect relationships and DPPH-HPLC-DAD analysis on nine cultivars of introduced rabbiteye blueberry in China

Sun, Li-Qiong; Ding, Xuehua; Qi, Jin; Yu, Hong; He, Shuqin; Zhang, Jian; Ge, Haixia; Yu, Boyang

doi:10.1016/j.foodchem.2011.11.030

Cited by 69 publications

(31 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DPPH-HPLC is a simple and rapid screening method that aids in the rapid selection of target components in complex extracts and has been widely used in many studies. [25][26][27] HSCCC is a unique separation technique based on the liquid-liquid partition chromatography without any solid support matrix. Therefore, it prevents the loss of sample observed in the conventional adsorption chromatography.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive evaluation of the antioxidant capacity of Perilla frutescens leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

Kwon

Wang

Hwang

et al. 2017

International Journal of Food Properties

View full text Add to dashboard Cite

To comprehensively examine the antioxidant capacity of Perilla frutescens leaves extract, eight assays including hydroxyl radical, hydrogen peroxide, ferrous ionchelating, total antioxidant capacity, nitric oxide, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2,2′-diphenyl-1-picrylhydrazyl, and ferrous reducing antioxidant assays were employed. The results indicated that the ethyl acetatesoluble fraction of P. frutescens leaves extract displayed the highest antioxidant activities. The antioxidant constituents from ethyl acetate-soluble fraction of P. frutescens leaves extract were screened by 2,2′-diphenyl-1-picrylhydrazyl highperformance liquid chromatography, and 11 compounds were identified after isolation using step-wise high-speed countercurrent chromatography. Protocatechuic acid, chlorogenic acid, caffeic acid, rosmarinic acid, luteolin, methyl-rosmarinic acid, apigenin, and 4′,5,7-trimethoxyflavone are the major antioxidant constituents in ethyl acetate-soluble fraction of P. frutescens leaves extract. ARTICLE HISTORY

show abstract

Section: Introductionmentioning

confidence: 99%

Comprehensive evaluation of the antioxidant capacity of Perilla frutescens leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

Kwon

Wang

Hwang

et al. 2017

International Journal of Food Properties

View full text Add to dashboard Cite

show abstract

“…Therefore, acetic acid-induced writhing test was used to evaluate analgesic activity of AT roots, with aspirin as positive drug. The higher the correlation coefficient is, the higher the contribution of the corresponding peak to analgesic activity of AT roots extract is [20]. The results (Table 5: correlation coefficient A) revealed that the contributions of 16 common peaks to analgesic activity of AT roots extract were in the order: peak 12 (atropine) > 8 (scopolamine) > 13 > 7 (scopolin) > 9 (anisodamine) > 11 > 10 > 4 > 2 (anisodine) > 5 > 15 > 6 (fabiatrin) > 1 (caffeoylputrescine) > 16 > 14 > 3.…”

Section: Discussionmentioning

confidence: 99%

Spectrum-effect relationships between high performance liquid chromatography fingerprint and analgesic property of Anisodus tanguticus (Maxim) Pascher (Solanaceae) roots

Jiang¹,

Zhong²,

Hu³

et al. 2017

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

“…The peak areas of antioxidants decreased or disappeared in the HPLC chromatogram after spiked with radicals, while for those without antioxidant activities, there was almost no change in their peak areas. The difference of the reduction of peak areas (PA) for radicals between the control sample and the experiment sample was used to evaluate radical scavenging activity of the sample according to the following equation [17][18][19] In this equation, PA control is the peak area of the control sample, and PA exp is the peak area of the experimental sample.…”

Section: Evaluation Of Radical Scavenging Capacitymentioning

confidence: 99%

Screening of active components as scavenger of radical from perennial fujimoto bean (Hyacinth Bean) whole herb by HPLC-MS coupled radical reaction

Shi

Wang

et al. 2016

Acta Chromatographica

View full text Add to dashboard Cite

Summary. High-performance liquid chromatography-mass spectrometry (HPLC-MS) method coupled with radical reaction for screening active ingredients from perennial fujimoto bean whole herb was established. The active ingredients, present in perennial fujimoto bean whole herb, possess scavenging effects towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide, peroxy radical, and hydroxyl radical. The radical scavenging abilities of these active ingredients were evaluated based on the relative peak areas in the HPLC chromatogram. The results indicate that potent antioxidants are present in the anhydrous methanol extract of perennial fujimoto bean whole herb. Based on HPLC-MS analysis, it was found that the scavenging ability can be mostly attributed to the presence of three compounds: cyanidin-3-o-β-D-glucopyranoside, troxerutin, and rutin. The structures were identified based on the MS and nuclear magnetic resonance (NMR) data. Free radical scavenging activity decreased in the following order: troxerutin > rutin > cyanidin-3-o-β-D-glucopyranoside.

show abstract

Antioxidant anthocyanins screening through spectrum–effect relationships and DPPH-HPLC-DAD analysis on nine cultivars of introduced rabbiteye blueberry in China

Cited by 69 publications

References 27 publications

Comprehensive evaluation of the antioxidant capacity of Perilla frutescens leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

Comprehensive evaluation of the antioxidant capacity of Perilla frutescens leaves extract and isolation of free radical scavengers using step-wise HSCCC guided by DPPH-HPLC

Spectrum-effect relationships between high performance liquid chromatography fingerprint and analgesic property of Anisodus tanguticus (Maxim) Pascher (Solanaceae) roots

Screening of active components as scavenger of radical from perennial fujimoto bean (Hyacinth Bean) whole herb by HPLC-MS coupled radical reaction

Contact Info

Product

Resources

About