Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells

Abstract

Delineation of factors which affect wound healing would be of immense value to enable on-time or early healing and reduce comorbidities associated with infections or biochemical stress like diabetes. Plasma gelsolin has been identified earlier to significantly enable injury recovery compared to placebo. This study evaluates the role of rhuGSN for its antioxidant and wound healing properties in murine fibroblasts (3T3-L1 cell line). Total antioxidant capacity of rhuGSN increased in a concentration-dependent manner (0.75-200 μg/mL). Cells pretreated with 0.375 and 0.75 μg/mL rhuGSN for 24 h exhibited a significant increase in viability in a MTT assay. Preincubation of cells with rhuGSN for 24 h followed by oxidative stress induced by exposure to H2O2 for 3 h showed cytoprotective effect. rhuGSN at 12.5 and 25 μg/mL concentration showed an enhanced cell migration after 20 h of injury in a scratch wound healing assay. The proinflammatory cytokine IL-6 levels were elevated in the culture supernatant. These results establish an effective role of rhuGSN against oxidative stress induced by H2O2 and in wound healing of 3T3-L1 fibroblast cells.

Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

“…Wound healing is a multifaceted biological process involving many extracellular and intracellular macromolecules. Healing occurs in four steps: hemostasis, inflammation, proliferation, and maturation ( Vaid et al, 2020 ), and has a vital role in skin remodeling after injury ( Mousavi et al, 2020 ). The economic evaluation of chronic wounds suggests that care and treatment of the wound are time-consuming and cost billions of dollars every year ( Nussbaum et al, 2018 ).…”

Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes

“…In the Scratch assay, scratches are created in vitro, causing the monolayer cell layer to deteriorate and the loss of cell-cell contact. Cell migration and proliferation are encouraged by the induction of various factors in the wound area over time [31,32]. Therefore, in our study, the scratch assay was used to evaluate the effect of B. spergulifolius extracts on cell migration and wound healing capacity in mature adipocytes.…”

“…Images of the scratch-wound were taken at 0. h, 6. h, 24. h, 30. h, 48. h using a microscope (4X magnification). Also, the scratch-wound area was measured from the digital images and calculated using ImageJ software (imagej.nih.gov) [32]. The scratch closure rate (%) was calculated:…”

“…In vitro scratch assay was used to evaluate the effects of MeOH, EA, Aqueous of B. spergulifolius extracts on the migration of mature 3T3-L1 adipocytes [31,32]. Cell migration and wound closure in untreated preadipocytes, adipocytes and adipocytes treated with IC 50 doses of MeOH, EA and Aqueous of B. spergulifolius extracts for 24 h were evaluated with the Scratch assay.…”

“…The percentage of the scratch wound closure was calculated using ImageJ software as the ratio between the scratch-wound area at the initial and at each time point of the assay [32]. According to data analysis of the percentage of the scratch wound closure, scratch-wound area of untreated adipocytes compared to pre-adipocytes decreased at 6 th , 24 th , 30 th and 48 th hour 50.88 ± 2.17%, 51.88 ± 2.03%, 2.94 ± 2.00%, and 0.33 ± 2.65% respectively (p = 0.0015, p = 0.0053, p = 0.3235, p = 0.4226).…”