Antioxidant and triglyceride-lowering effects of vitamin E associated with the prevention of abnormalities in the reactivity and morphology of aorta from streptozotocin-diabetic rats

Karasu, Çi̇men; Ozansoy, Gülgun; Bozkurt, Onur; Erdoğan, Deniz; Ömeroğlu, Suna

doi:10.1016/s0026-0495(97)90072-x

Cited by 71 publications

(65 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microscopic evaluation of the aorta was performed according to the protocol described by previous study (Karasu et al, 1997). Following dissection, the thoracic aorta was cut into small pieces which then fixed at 4˚C for 2 hours in 2.5% buffered glutaraldehyde and post-fixed for 90 minutes with osmium tetroxide.…”

Section: Transmission Electron Microscopic Evaluationmentioning

confidence: 99%

Hibiscus sabdariffa Linn. improves the aortic damage in diabetic rats by acting as antioxidant

Zainalabidin¹,

Budin²,

Anuar³

et al. 2018

J App Pharm Sci

View full text Add to dashboard Cite

Chronic hyperglycaemia produces excessive free radicals which could lead to diabetic complications. Hibiscus sabdariffa Linn. is proven to have high antioxidant and antihyperlipidemic properties. The aim of the study was to investigate the effects of H. sabdariffa polyphenol-rich extract (HPE) in the aorta of diabetic rats. Twenty-four male rats were randomly divided into three groups, i.e. non-diabetes mellitus (NDM), diabetes mellitus (DM) and diabetes mellitus with HPE (DM + HPE). HPE at 100 mg/kg or saline as control vehicle were administered daily through oral gavage for 28 days prior to sacrification and the aorta was isolated for further analysis. The glucose level in DM + HPE was significantly lower compared to DM, indicating its potential as an antidiabetic agent. Conversely, catalase (CAT), superoxide dismutase (SOD) activity and the level of glutathione (GSH) in DM + HPE were significantly higher than DM. The level of malondialdehyde (MDA) and protein carbonyl were significantly lower in DM + HPE than DM. Lipid profile analysis demonstrated a significant decrease in triglyceride, total cholesterol and LDL-C in DM + HPE compared to DM, however, HDL-C was significantly increased. Microscopic observation showed a disrupted morphology of the thoracic aorta in diabetic rats. HPE may exert protective effects by acting as an antidiabetic, antihyperlipidemic and antioxidant agent to the thoracic aorta in diabetic rats.

show abstract

Section: Transmission Electron Microscopic Evaluationmentioning

confidence: 99%

Hibiscus sabdariffa Linn. improves the aortic damage in diabetic rats by acting as antioxidant

Zainalabidin¹,

Budin²,

Anuar³

et al. 2018

J App Pharm Sci

View full text Add to dashboard Cite

show abstract

“…Hyperglycemia is known to causes increased production of free radicals and insufficiencies in the antioxidant system [2]. Excess reactive oxygen species (ROS) is secreted in the inflammatory phase of wound healing by neutrophils and macrophages [3].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of d-δ-tocotrienol rich fraction on excisional skin wounds in diabetic rats

Elsy¹,

Khan²,

Maheshwari³

2017

Our Dermatol Online

View full text Add to dashboard Cite

“…29,30 Vitamin E has also been reported to improve NO-mediated arterial relaxation, maintain cell membrane integrity and protein stability. 31,32 The potential impact of combining vitamin E and sildenafil to treat diabetes induced ED (ED-DM) remains to be determined. This animal study was designed to gain insight into the potential clinical utility of this approach.…”

Section: Introductionmentioning

confidence: 99%

Effect of PDE5 inhibition combined with free oxygen radical scavenger therapy on erectile function in a diabetic animal model

Young

Freeman

et al. 2003

Int J Impot Res

View full text Add to dashboard Cite

Phosphodiesterase (PDE) inhibitors represent an important advance in the treatment of erectile dysfunction (ED). In spite of widespread use and generally good efficacy, as a class they remain ineffective in 15-57% of men. Specific cohorts of patients with severe vascular or neurogenic basis to their ED, such as diabetic men or those who have undergone radical pelvic surgery, demonstrate lower response rates with PDE inhibition treatment. We believe that circulating levels of nitric oxide (NO) may be enhanced through delivery of adequate concentrations of free oxygen radical scavenger molecules such as vitamin E. Higher levels of NO, theoretically, should produce increased penile blood flow with the potential for a synergistic effect when combined with a PDE5 inhibitor. With this hypothesis in mind, 20 adult male Sprague-Dawley streptozotocin-induced (60 mg/kg i.p.) diabetic rats were divided into four therapeutic groups (n ¼ 5). Group IFcontrol animals received peanut oil, group IIFvitamin E 20 IU/day, group IIIFsildenafil 5 mg/kg/day and group IVFvitamin E 20 IU/day plus sildenafil 5 mg/kg/day, by oral gavage daily for 3 weeks. Erectile function was assessed as a rise in intracavernous pressure following cavernous nerve electrostimulation. Penile tissue was harvested to determine the changes in tissue morphology including neuronal nitric oxide synthase, smooth muscle a-actin and endothelial cell integrity. PDE5 protein content and activity were measured. Significant increases in intracavernous pressure were measured in the animals receiving combined vitamin E plus sildenafil treatment. Immunohistochemical staining showed increases of neuronal nitric oxide synthase, endothelial cell and smooth muscle cell staining. Western blot analysis did not show significant differences of PDE5 protein between the groups. However, higher PDE5 activity was measured in the sildenafil group and lower activity of PDE5 was recorded in the cohort receiving vitamin E with sildenafil. Vitamin E enhanced the therapeutic effect of the PDE5 inhibitor in a meaningful way in this animal model of diabetes. This study indicates a potential means of salvaging erectile function among patients who are refractory to sildenafil.

show abstract

Antioxidant and triglyceride-lowering effects of vitamin E associated with the prevention of abnormalities in the reactivity and morphology of aorta from streptozotocin-diabetic rats

Cited by 71 publications

References 45 publications

Hibiscus sabdariffa Linn. improves the aortic damage in diabetic rats by acting as antioxidant

Hibiscus sabdariffa Linn. improves the aortic damage in diabetic rats by acting as antioxidant

Therapeutic potential of d-δ-tocotrienol rich fraction on excisional skin wounds in diabetic rats

Effect of PDE5 inhibition combined with free oxygen radical scavenger therapy on erectile function in a diabetic animal model

Contact Info

Product

Resources

About