Antioxidant and prooxidant effects of Piptadeniastrum africanum as the possible rationale behind its broad scale application in African ethnomedicine

Dlamini, Lindiwe M.; Tata, Charlotte Mungho; Djuidje, Marthe Carine F.; Ikhile, Monisola I.; Nikolova, Galina; Karamalakova, Yanka; Gadjeva, V.; Zheleva, A.; Njobeh, Patrick Berka; Ndinteh, Derek Tantoh

doi:10.1016/j.jep.2018.11.039

Cited by 13 publications

(11 citation statements)

References 59 publications

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“…The antioxidant property of polyphenolic compounds has been reported [25][26]. A correlation between antioxidant activity and the phenolic content has been documented [24,27]. Plant extracts with high phenolic content also show high avonoid content [28] as evident in this study.…”

Section: Resultssupporting

confidence: 67%

Antioxidant Potentials of the Pod Extract of Caesalpinia Pulcherrima Swarta (Fabaece) and the Theoretical Evaluation of the Antioxidant Property of the Isolated Compounds

Faloye

Famuyiwa

Akinwunmi

et al. 2021

Preprint

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Various diseases associated with oxidative stress have necessitated the need to investigate plants for antioxidant agents. This study aims to investigate the antioxidant potential of the extract and fractions of C. pulcherrima and estimate the quantum chemical properties of polyphenolic compounds isolated from its most active antioxidant fraction. Methanolic extract and partition fractions of the pods of the plant were assayed for their antioxidant activity using four models: 1,1-diphenyl-2-dipicrylhydrazyl radical scavenging, ferric reducing antioxidant power, total antioxidant capacity and hydroxyl radical scavenging activity. Total phenolic and flavonoid contents of the extract and fractions were also investigated. Furthermore, the quantum chemical properties of two polyphenolic compounds were calculated to predict the antioxidant potential. The extract exhibited good antioxidant activity with IC50 = 45.63 µg/ml comparable to ascorbic acid of IC50 = 37.94 µg/ml, high reducing power with value at 376.74 ± 6.78, total antioxidant capacity obtained at ascorbic acid equivalence of 383 ± 8.16 mgAAE/g and potent hydroxyl radical scavenging activity with value at 580.97 µg/ml with ascorbic acid at 30.7 µg/ml. The total phenol and flavonoid contents values at 540.53 ± 3.71 and 347.87 ± 7.13 respectively. Furthermore, the results obtained from the quantum chemical calculations reveals polyphenolic compounds; 5(4-hydroxyphenyl)-3-hydroxy-2-methoxyphenol and 3(4-methanetriiol-2,6-dihydroxyphenoxy)-3’, 4’, 5’, 5, 7-pentahydroxyflavonol as promising antioxidant agents. The study concluded that C. pulcherrima pods have good antioxidant activity. Also, the polyphenolic compounds are among the chemical constituents responsible for the antioxidant activity obtained for the extract and ethyl acetate fraction.

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Section: Resultssupporting

confidence: 67%

Antioxidant Potentials of the Pod Extract of Caesalpinia Pulcherrima Swarta (Fabaece) and the Theoretical Evaluation of the Antioxidant Property of the Isolated Compounds

Faloye

Famuyiwa

Akinwunmi

et al. 2021

Preprint

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“…Piptadeniastrum africanum (Hook.f.) Brenan (Mimosaceae), a massive tree which can reach up to 50 m, is widely distributed across Western Africa tropical rainforests of Angola, Uganda, Senegal Cameroon, Democratic Republic of Congo, Nigeria, and Sudan [1]. P. africanum has been extensively used in African traditional medicinal systems.…”

Section: Introductionmentioning

confidence: 99%

“…Dlamini and colleagues (2019) summarized the ethnomedicinal uses of P. africanum across different countries in Africa. As such, they reported that in Nigeria the stem bark and root were used to treat sickle cell anemia and cancer, respectively; in Guinea the stem bark is used for its antibacterial properties, whereas leaves are used in the management of diabetes; in Ghana, Benin, and Gabon the bark is used for waist pain impotence [1].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts

et al. 2020

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The stem bark (SB) of Piptadeniastrum africanum (PA) has been extensively used in African traditional medicinal systems. However, there is a dearth of scientific information regarding its possible activity in the management of type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. This study therefore attempted to elucidate the in vitro inhibitory action of ethyl acetate, methanol, and water extracts of P. africanum stem bark (PA-SB) on α-amylase, α-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase. Cell viability, catecholamine, and 3-hydroxykynurenine levels of hypothalamic HypoE22 cells exposed to PA-SB extracts were also investigated. The phytochemical profiles of the extracts were determined by high performance liquid chromatography (HPLC) and antioxidant properties were investigated. Saponin (867.42 mg quillaja equivalent/g) and tannin (33.81 mg catechin equivalent/g) contents were higher in the methanol extract. Multiple dihydroxy-trimethoxy(iso)flavone isomers, loliolide, eriodictyol, naringenin, luteolin, chrysoeriol, apigenin, and liquiritigenin, were characterized from PA-SB extracts using HPLC. The methanol extract of PA-SB showed highest inhibitory activity against acetylcholinesterase (4.88 mg galantamine equivalent (GALAE)/g extract), butyrylcholinesterase (5.37 mg GALAE/g extract), and tyrosinase (154.86 mg kojic acid equivalent/g extract) while α-glucosidase was effectively inhibited by the ethyl acetate extract (15.22 mmol acarbose equivalent/g extract). The methanol extract of PA-SB also showed potent antioxidant properties (493.87, 818.12, 953.07, and 732.19 mg Trolox equivalent/g extract, for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays, respectively). PA-SB extracts exhibited antioxidant activity and promising inhibition against key enzymes related to type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. Additionally, all extracts were able to contrast hydrogen peroxide-induced oxidative stress, in HypoE22 cells, thus restoring basal catecholamine and 3-hydroxykinurenine levels, whereas only methanol and water extracts stimulated basal dopamine release. Overall, data from the present study contribute to the biological assessment of P. africanum that appears to be a promising source of natural compounds with protective and neuromodulatory effects.

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“…Even though polyphenols present antioxidant activity, with numerous benefits in preserving human health, the pro-oxidant effect of these compounds must be recognized [ 75 , 76 , 77 ]. When consumed in low or moderate concentrations, pro-oxidants can be considered as weapons for the defense system of the organism and if consumed in high doses, they have toxic potential by causing OS.…”

Section: Discussionmentioning

confidence: 99%

“…When consumed in low or moderate concentrations, pro-oxidants can be considered as weapons for the defense system of the organism and if consumed in high doses, they have toxic potential by causing OS. On the other hand, the ability of pro-oxidants to produce cell apoptosis can be exploited in cancer therapy [ 76 , 77 , 78 ]. Thus, polyphenol-rich dietary supplements could offer health benefits to consumers when administered in appropriate doses [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing

et al. 2022

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Grape pomace and berries represent natural sources of phytochemicals that can increase the quality of life of consumers by contributing to the prevention of chronic diseases; thus, the development of a dietary supplement was necessary. The raw material (r.m.) used for the development of the dietary supplement consisted of dried and powdered bilberries (Vaccinium myrtillus L.), red currants (Ribes rubrum L.), and red fermented pomaces (Vitis vinifera L.) from Feteasca Neagra and Cabernet Sauvignon cultivars. The particle size distribution, powder flow, total phenolic content (TPC), HPLC-DAD phenolic profile assessment, and radical scavenging assay (RSA) were employed for the analysis of the raw material. After encapsulation, the average mass and uniformity of mass, the disintegration, and the uniformity of content for the obtained capsules were performed to obtain a high-quality dietary supplement. All the assays performed complied to the compendial requirements and the TPC was determined at 9.07 ± 0.25 mg gallic acid equivalents/g r.m. and RSA at 48.32 ± 0.74%. The highest quantities of phenolic compounds determined were 333.7 ± 0.50 µg/g r.m. for chlorogenic acid, followed by rutin, ferulic acid, and (+)-catechin with 198.9 ± 1.60 µg/g r.m., 179.8 ± 0.90 µg/g r.m. and 118.7 ± 0.75 µg/g r.m., respectively. The results of this study can be used for the manufacturing and assessing of pilot scale-up capsule batches and thinking of quality assurance, we recommend that the industrial batch extracts should be standardized in polyphenols, and the manufacturing process should be validated.

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Antioxidant and prooxidant effects of Piptadeniastrum africanum as the possible rationale behind its broad scale application in African ethnomedicine

Cited by 13 publications

References 59 publications

Antioxidant Potentials of the Pod Extract of Caesalpinia Pulcherrima Swarta (Fabaece) and the Theoretical Evaluation of the Antioxidant Property of the Isolated Compounds

Antioxidant Potentials of the Pod Extract of Caesalpinia Pulcherrima Swarta (Fabaece) and the Theoretical Evaluation of the Antioxidant Property of the Isolated Compounds

Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts

Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing

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