Antioxidant and Hepatoprotective Effects of Croton hypoleucus Extract in an Induced-Necrosis Model in Rats

Abstract

The aim of this study was to evaluate the antioxidant and hepatoprotective activity of Croton hypoleucus (EC). The present work reports the first pharmacological, toxicological, and antioxidant studies of EC extract on liver injury. Liver necrosis was induced by thioacetamide (TAA). Five groups were established: Croton Extract (EC), thioacetamide (TAA), Croton extract with thioacetamide (EC + TAA), vitamin E with thioacetamide (VE + TAA) and the positive control and vehicle (CT). For EC and EC + TAA, Wistar rats (n = 8) were intragastrically pre-administered for 4 days with EC (300 mg/kg.day) and on the last day, EC + TAA received a single dose of TAA (400 mg/kg). At 24 h after damage induction, animals were sacrificed. In vitro activity and gene expression of superoxide dismutase (SOD), catalase (Cat), and Nrf2 nuclear factor were measured. The results show that EC has medium antioxidant properties, with an IC50 of 0.63 mg/mL and a ferric-reducing power of 279.8 µM/mg. Additionally, EC reduced hepatic damage markers at 24 h after TAA intoxication; also, it increased SOD and Cat gene expression against TAA by controlling antioxidant defense levels. Our findings demonstrated the hepatoprotective effect of EC by reducing hepatic damage markers and controlling antioxidant defense levels. Further studies are necessary to identify the mechanism of this protection.

In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide

“…A single dose of TAA could produce centrilobular hepatic necrosis whereas chronic administration led to cirrhosis 11 . Several researchers reported that TAA has been an effective agent to induce liver fibrosis in experimental animals 12–17 …”

“…11 Several researchers reported that TAA has been an effective agent to induce liver fibrosis in experimental animals. [12][13][14][15][16][17] The numbers of medicinal/therapeutic plants were characterized and proved to be effective in hepatoprotective action. Some of them include, Sappan wood (Caesalpinia sappan L.), Ginger (Zingiber officinale), Java tea (Orthosiphon stamineus), Cucumis ficifolius, and Chinese chastetree (Vitex negundo).…”

Combination of Trans-Resveratrol and ε-Viniferin Induces a Hepatoprotective Effect in Rats with Severe Acute Liver Failure via Reduction of Oxidative Stress and MMP-9 Expression

“…Other studies using TAA reported similar results [10,29]. Studies using resveratrol as an antioxidant, but in different models, also reported identical results [30]. As in other studies using flavonoids [17] and vitamin E [27], the administration of resveratrol and ε-viniferin reduced the activity of SOD compared to TAA-treated rats, achieving values similar to the controls, possibly in an attempt to adjust for the liver damage caused by the presence of the xenobiotic (Figure 3A).…”

Exenatide improves random‐pattern skin flap survival via TFE3 mediated autophagy augment

“…Ischemia‐reperfusion injury is another cause of flap necrosis as ROS accumulate during reperfusion, leading to oxidative stress and cell damage (Chen et al, 2018; Zhao et al, 2019). SOD, an antioxidant enzyme, acts as a first line of defense against oxidative damage and neutralizes superoxide radicals to hydrogen peroxide (H 2 O 2 ) and oxygen (Urrutia‐Hernández et al, 2019; L. Zhang et al, 2016). Other proteins, such as HO1 and eNOS, also exert antioxidant effects (Sun et al, 2018).…”