Combination of Trans-Resveratrol and ε-Viniferin Induces a Hepatoprotective Effect in Rats with Severe Acute Liver Failure via Reduction of Oxidative Stress and MMP-9 Expression

Abstract

Stilbenes are a major grapevine class of phenolic compounds, known for their biological activities, including anti-inflammatory and antioxidant, but never studied in combination. We aimed to evaluate the effect of trans-resveratrol + ε-viniferin as an antioxidant mixture and its role in inflammatory development an in vivo model of severe acute liver failure induced with TAA. Trans-resveratrol + trans-ε-viniferin (5 mg/kg each) was administered to Wistar rats. Resveratrol + ε-viniferin significantly decreased TBARS and SOD activity and restored CAT and GST activities in the treated group. This stilbene combination reduced the expression of TNFα, iNOS, and COX-2, and inhibited MMP-9. The combination of resveratrol + ε-viniferin had a hepatoprotective effect, reducing DNA damage, exhibiting a protective role on the antioxidant pathway by altering SOD, CAT, and GST activities; by downregulating TNFα, COX-2, and iNOS; and upregulating IL-10. Our results suggested that adding viniferin to resveratrol may be more effective in hepatoprotection than resveratrol alone, opening a new perspective on using this stilbene combination in functional diets.

Apigenin role against thioacetamide-triggered liver fibrosis: Deciphering the PPARγ/TGF-β1/NF-κB and the HIF/FAK/AKT pathways

“…Our findings demonstrated that TAA significantly increased the levels of NF-κB and TGF-β1. TAA triggers the transcription of NF-κB, which activates the production of proinflammatory cytokines, such as TNFα and IL-1β, and the modulation of IL-10 anti-inflammatory cytokine (38). The antiinflammatory activity of APG was demonstrated by lower levels of TNF-α, IL-1β, NF-κB, and TGF-β1, as well as higher levels of IL-10, implying that APG reduces liver fibrosis by limiting the levels of inflammatory cytokines and exerting its protective effect by inhibiting the NF-κB and TGF-β1 pathways.…”

Lupin Protein Concentrate as a Novel Functional Food Additive That Can Reduce Colitis-Induced Inflammation and Oxidative Stress

“…In our study, we observed a significant increase in SOD activity in the colitis group ( Figure 3 C), most likely to compensate for the damage caused by the action of acetic acid in the animals’ intestines. The enzyme SOD has an essential role in cellular redox balance, promoting dismutation in an attempt to free radicals, protecting tissues against oxidative damage [ 28 ]. However, whilst the Col + Cc group presented similar levels ( p < 0.001) of SOD activity when compared to the colitis groups, the treated group showed a significant decrease in SOD activity, even reaching levels that were significantly similar to the control (healthy) group.…”

“…The activity of superoxide dismutase (SOD), defined by its ability to inhibit the reaction of superoxide radicals with adrenaline, was monitored spectrophotometrically at 560 nm. Results were expressed as USOD/mg protein [ 28 ]. The activity of glutathione peroxidase (GPx) was assessed by the NADPH oxidation rate in the presence of reduced glutathione and glutathione reductase.…”

“…Sodium azide was added to inhibit catalase activity. GPx activity was measured spectrophotometrically at 340 nm and expressed as nmol/min/mg protein [ 28 ].…”

Early prediction of bronchopulmonary dysplasia: can noninvasive monitoring methods be essential?

“…Matrix metalloproteinases (MMPs) have crucial roles in a variety of pathological processes, including inflammation, cardiovascular problems and respiratory system disorders [ 42 ]. Tissue inhibitors of metalloproteinases (TIMPs) regulate the action of MMPs.…”