Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats

Pareek, Anil; Godavarthi, Ashok; Issarani, Roshan; Nagori, Badri Prakash

doi:10.1016/j.jep.2013.09.048

Cited by 97 publications

(70 citation statements)

References 51 publications

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“…Further, IC 50 values obtained for DPPH scavenging assay also produced significant association (R 2 = 0.8551; p <0.05, R 2 = 0.7100; p <0.05) with the total flavonoid and total phenolic constituents. Our results are endorsed by a previous study where the aqueous fraction having gallic acid exhibited the strong antioxidant activity [3, 18, 49]. …”

Section: Discussionsupporting

confidence: 88%

“…Antipyretic activity in rat has been reported for F. indica [17]. Pareek et al [18] investigated the i n vitro and in vivo antioxidant activity of F. schweinfurthi through DPPH, ABTS radicals and hydrogen peroxide methods and hepatoprotective potential against CCl 4 induced toxicity in HepG2 cells and in rat. Hepatoprotective activity of F. indica has also been reported in earlier studies [19, 20].…”

Section: Introductionmentioning

confidence: 99%

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Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

Rashid¹,

Khan

Sajid

2016

BMC Complement Altern Med

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Background Fagonia olivieri (DC) being used for the treatment of diabetes, cancer, fever and claimed to be effective in many other stress related disorders. In this study we have evaluated the F. olivieri whole methanol extract and its derived fractions for various in vitro and in vivo antioxidant studies.MethodsThe crude methanol extract of the whole plant of F. olivieri (FOM) and its derived fractions; n-hexane (FOH), chloroform (FOC), ethyl acetate (FOE), n-butanol (FOB) and aqueous (FOA) were evaluated for the total phenolic and flavonoid content and in vitro antioxidant abilities. The antioxidant effect of FOM was determined by acetaminophen-induced hepatotoxicity in Sprague–Dawley (Rattus novergicus) male rats. The methanol/fractions were also analysed by HPLC analysis for the presence of polyphenolics.ResultsThe total phenolic content of the samples ranged from 19.3 ± 0.529 to 106.2 ± 0.892 mg GAE/g extract while total flavonoid content 16.2 ± 0.881 to 50.1 ± 1.764 mg RTE/g extract, respectively. FOA showed highest radical scavenging activity for DPPH (IC50 = 55.2 ± 1.212 μg/ml), ABTS (IC50 = 90.2 ± 1.232 μg/ml) superoxide (IC50 = 37.1 ± 0.643 μg/ml) and for H2O2 (IC50 = 64 ± 1.463 μg/ml). FOE exhibited the highest antioxidant activities for phosphomolybdenum (IC50 = 78.2 ± 0.883 μg/ml) and for hydroxyl radical scavenging (IC50 = 82 ± 2.603 μg/ml). HPLC analysis of FOM and its derived fractions showed the presence of rutin, catechin and gallic acid. Elevated levels of AST, ALT, ALP, LDH and lipid profile in serum and lipid peroxidation and DNA damages in liver; while decreased activity level of CAT, SOD, GSH-Px, GR and reduced glutathione (GSH) concentration induced with acetaminophen in rat were reverted towards the control group with co-administration of FOM.ConclusionOur results showed that F. olivieri is a potential source of natural antioxidants, which justifies its use in folklore medicine.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

Rashid¹,

Khan

Sajid

2016

BMC Complement Altern Med

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“…Presently, the research community is focusing on thoroughgoing pharmacological validation and identification of biomarkers for the treatment of various disease conditions (Feng et al, 2011; Pareek et al, 2013; Choudhury B. et al, 2016). North Eastern India is a part of Indo-Burma biodiversity hotspot known for widespread availability of herbal medicine.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Bioactivity Guided Fractions of Ziziphus jujuba Mill. Root Bark against Hepatic Injury and Chronic Inflammation via Inhibiting Inflammatory Markers and Oxidative Stress

Kandimalla

Dash²,

Kalita

et al. 2016

Front. Pharmacol.

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The tribal communities of North Eastern India rely on herbal medicine to cure various disease conditions. Ziziphus jujuba Mill. (Rhamnaceae) is one of such medicinal plants used for curing liver ailments, insomnia, anemia, diarrhea, diabetic complications, cancer, and loss of appetite. The present study was aimed to describe the protective ability of Z. jujuba root bark (ZJRB) against hepatic injury and chronic inflammation. Bioactivity guided fractionation of Z. jujuba methanol extract (ZJME) was performed using different solvents of increasing polarity viz. hexane (ZJHF), chloroform (ZJCF), ethyl acetate (ZJEAF), water (ZJWF), and residue (ZJMR). In vitro antioxidant results revealed that both ZJME and ZJWF possess strong antioxidant activity among all the fractions and mother extract tested. Further, ZJME and ZJWF showed significant protection against CCl4 intoxicated HepG2 cell lines by means of increased cell viability and decreased LDH levels compared to control group. ZJME at 200, 400 mg/kg and ZJWF at 50, 100 mg/kg inhibited the lipid peroxidation and significantly restored the liver function markers (AST, ALT, ALP, LDH, SOD, and CAT) and cytokine levels (TNF-α, Il-1β, and Il-10) in CCl4 induced acute liver damage in rats. All the results were comparable with standard drug silymarin which was further confirmed by histopathology analysis of liver. Similarly, inflammation and increase inflammatory cytokines levels of carrageenan induced paw edema in rats have been refurbished to normal levels on par with the standard drug indomethacin. ZJWF demonstrated potent response than ZJME in all the biological tests conducted. The results of the study signify the ability of ZJRB as good therapeutic agent for liver toxicity and chronic inflammation.

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“…Livers were removed from the rats and immediately washed with salt (0.9% sodium chloride) in cold conditions. The liver tissues were dried and weighed as much as 100 mg (10%) for analysis of oxidative stress parameters (Pareek et al, 2013).…”

Section: In Vivo Hepatoprotective Activity Testingmentioning

confidence: 99%

“…The homogenates were centrifuged at 4°C temperature at 10.000rpm for 10min to obtain supernatants. The supernatants were used for analysis of oxidative stress parameters Including MDA (malondialdehyde), GSH (glutathione) and CAT (catalase) (Pareek et al, 2013).…”

Section: Estimation Of Oxidative Stress Parametersmentioning

confidence: 99%

Hepatoprotective Activity of Ethyl Acetate Fraction of Senggugu’s Root Bark (Clerodendrum serratum L.Moon) on Rats Induced by CCl4

Nasrudin

Wahyono

Mustofa

et al. 2017

Indonesian J. Pharm.

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Our previous study showed that ethyl acetate fraction of Clerodendrum serratum L. Moon (EAFCS) root bark had in vitro antioxidant activity. The aim of this study was to evaluate hepatoprotective effect of EAFCR in rats induced by carbon tetrachloride (CCl 4 ). Thirty-six rats were randomly divided into 6 groups. Group 1 as control was given 0.5% Na-CMC. Group 2 as positive control were given silymarin at a dose of 100mg/Kg.BW. Group 3 as negative control were induced by CCl 4 . Group 4-6 as treatment groups were induced CCl4 and given EAFCR at a dose of 25; 50 and 100mg/Kg BW, respectively. Biochemical and oxidative stress parameters in liver were determined. The results showed that serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and bilirubin were significantly lower, whereas the total protein was significantly higher after pretreatment with EAFCR at the dose of 100mg/Kg.BW (p<0.05). Moreover, malondialdehyde (MDA) was significantly lower, whereas glutathione peroxidase (GPx) and catalase (CAT) were significantly higher after pretreatment with EAFCS at the dose of 100mg/Kg.BW (p<0.05). In conclusion, EAFCR has potent hepatoprotective activity due to its antioxidant properties of the active compounds contained in this plant.

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Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats

Cited by 97 publications

References 51 publications

Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

Protective Effect of Bioactivity Guided Fractions of Ziziphus jujuba Mill. Root Bark against Hepatic Injury and Chronic Inflammation via Inhibiting Inflammatory Markers and Oxidative Stress

Hepatoprotective Activity of Ethyl Acetate Fraction of Senggugu’s Root Bark (Clerodendrum serratum L.Moon) on Rats Induced by CCl4

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