Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carotassp.carota)

Abstract

Context: Wild carrot, Daucus carota L. ssp. carota (Apiacae), is widely distributed throughout the world and has various uses in traditional medicine in Lebanon.Objective: The present study aimed to fractionate and analyze the chemical composition of the Daucus carota oil extract (DCOE) fractions and to evaluate their antioxidant and hepatoprotective properties in vitro and in vivo. Materials and methods: DCOE was chromatographed on silica gel column to produce four fractions: pentane (F1), 50:50 pentane:diethyl ether (F2), diethyl ether (F3), and 93:7 chloroform: methanol (F4). Qualitative and quantitative analyses of oil fractions were performed by GC-MS and HPLC techniques. The in vitro antioxidant properties were assessed using DPPH, FIC, and ferric-reducing antioxidant power (FRAP) assays. The hepatoprotective property was determined by examining the levels of serum markers (alanine transaminase (ALT) and aspartate transaminase (AST)) and hepatic antioxidant (superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST)) enzymes in CCl 4 -intoxicated mice pretreated with intraperitoenal 50, 100, or 200 mg/kg b.w. of the oil fractions for 5 d. Results: GCMS analysis of F2 revealed the presence of 2-himachalen-6-ol (61.4%) which is reported for the first time in Daucus carota species. F3 and F4 were rich in phenolics and flavonoids and demonstrated significant DPPH activity (IC 50 ¼ 0.29 and 0.38 mg/ml, respectively) and high FRAP values (225.11 and 437.59 mmol FeSO 4 /g, respectively). The sesquiterpene-rich fraction F1 had the highest FIC ability (IC 50 ¼ 0.28 mg/ml). Pretreatment with F1 and F4 reversed the CCl 4 -induced decrease in SOD, CAT, and GST levels and reduced significantly hepatic damage. Discussion and conclusion: The current results suggested that wild carrot oil fractions exhibited a unique chemical composition and possessed significant antioxidant activities as well as hepatoprotective effects against CCl 4 -induced hepatotoxicity.

Wild carrot pentane-based fractions suppress proliferation of human HaCaT keratinocytes and protect against chemically-induced skin cancer

“…These results are consistent with previous work conducted in our laboratory using unfractionated DCOE [4]. In addition, F2 fraction was shown to markedly increase the level of glutathione S-transferase (GST) in CCl4-treated mice [17], an enzyme involved in the detoxification of carcinogens and in turn protect cellular components against toxic compounds and chemical carcinogenesis [43]. Therefore, the chemopreventive action of F2 against DMBA/TPA-induced skin carcinogenesis may be partly attributed to this enhanced activity of GST.…”

“…Fractions were analyzed by TLC using hexane:ethyl acetate (70:30) as mobile phase and plates were stained with 2% anisaldehyde. The chemical components of various fractions were determined as previously described [17]. …”

Medicinal Plants Used in Traditional Management of Cancer in Uganda: A Review of Ethnobotanical Surveys, Phytochemistry, and Anticancer Studies

“…α-Longipinene, methyl isoeugenol, elemicin, β-Selinene, β-asarone, β-himachalene, β-bisabolene, α-humulene, widdrol, allo-aromadendrene, α-caryophyllene, β-caryophyllene, caryophyllene oxide, aromadendrene [126], 2-himachalen-6-ol, (+)-a-longipinene, longicyclene, and β-caryophyllene [127] Chemopreventive against 7,12-dimethyl Benz(a) anthracene-induced squamous cell carcinoma in mice [126]. e aqueous extract has anticancer activity against human promyelocytic leukemia HL-60 cells.…”

“…Treating skin diseases and wounds [34], fatigue [33], cough, diarrhea, dysentery, malaria, as an antiseptic, abortifacient, aphrodisiac, carminative, stimulant, stomachic, and tonic [209] Antioxidant and hepatoprotective activities [127] Elaeodendron buchananii (Loes) Loes.…”

β-2-himachalen-6-ol: A novel anticancer sesquiterpene unique to the Lebanese wild carrot

“…The Daucus carota oil extract (DCOE) was then found to possess antioxidant and anticancer activities against Caco-2, HT-29, MCF-7 and MB-MDA-231 (Shebaby et al, 2013), human acute myeloid leukemia cells (Tawil et al, 2014), and protect against chemically induced skin carcinogenesis (DMBA-TPA) in mice (Abu Zeinab et al, 2011). Recently, DCOE was subjected to a chromatographic separation yielding 4 fractions whose antioxidant (Shebaby et al, 2015b) and hepatoprotective (Shebaby et al, 2015b) activities were investigated. In addition, the fractions were tested for their anticancer activity against various cancer cell lines (Shebaby et al, 2014;Shebaby et al, 2015a).…”