Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl.

Abstract

Introduction: In recent years, natural antioxidants have seen an unprecedented importance and demand in bio-pharmaceuticals, nutraceuticals besides their use as food additives. Antioxidants act as potential prophylactic and therapeutic agents against various diseases caused by free-radicals. Plants offer tremendous source of antioxidants and are therefore being evaluated for their potentials. Eulophia nuda is an important medicinal plant used by local healers in India; however its antioxidant properties have not yet been investigated. Methods: Aqueous (AqE), methanol (ME), aqueousemethanol (AqME) and acetone (AE) extracts of shade dried tubers were obtained and were concentrated in vacuo. Total phenols, flavonoids, ascorbic acid and carotenoids were estimated from all extracts using standard methods. Antioxidant activities of extracts were determined by total antioxidant activity, FRAP, ABTS, DPPH, and OH radical scavenging assays besides lipid peroxidation inhibition. Extracts were evaluated for protection of Fenton's reagent induced DNA damage. Results: The results confirmed the plant as a rich source of phenols, flavonoids, vitamin C and carotenoids. Among four extracts, AqME showed highest antioxidant activities as evidenced by maximum scavenging of ABTS (98%), DPPH (87%), and OH radicals (99%) at 1 mg ml À1 concentration and showed maximal inhibition of lipid peroxidation. All extracts protected the DNA from hydroxyl-radical-induced damage. Again, AqME was proved to be best in providing protection to DNA against damage caused by freeradicals. Conclusion: The results provides scientific basis for its traditional usage as natural antioxidant and phytotherapeutic agent. The plant possesses high amount of phenolic compounds and showed a broadspectrum antioxidant properties including DNA protection.

Synthesis and Antioxidant Properties of New Substituted 8‐Methyl‐6‐phenyl‐5,6‐dihydro‐4H‐1,3,2‐benzodioxaphosphocine‐2‐oxide Derivatives

“…H 2 SO 4 under nitrogen atmosphere at 120°C for a period of 6 h to afford 3,4-dihydro-6-methyl-4-phenyl-2H-benzopyran-2-one (3). Reduction of 3 with NaBH 4 in methanol produced 2-(3-hydroxy-1-phenylpropyl)-4-methylphenol (4). Phosphorylation of 4 with P(O)Cl 3 afforded the monochloride (5), which was subsequently condensed with amines to obtain the 2-amino derivatives of the title compounds 6a-j.…”

“…Synthesis of 2-(3-hydroxy-1-phenylpropyl)-4-methylphenol (4). Two grams of compound 3 was taken in a 100-mL round bottomed flask.…”

In vitro antioxidant and free radical scavenging activities of stem extract of Euphorbia trigona Miller

“…They reported values of phenolic compounds between 10.8 and 56.5 mg/ g DW of herb. Kumar et al (2013) reported that the total phenolic content of Eulophia nuda in acetone and methanol extracts were higher than in aqueous extract. Our results also largely supported these conclusions.…”

Hypoglycaemic Effect of Glibenclamide: A Critical Study on the Basis of Creatinine and Lipid Peroxidation Status of Streptozotocin-induced Diabetic Rat

“…A small quantity of the above two homogenates were separately mixed with 10% trichloroacetic acid (TCA), shaken well, centrifuged and the precipitate was used to estimate the total protein in the tissues. LPO product MDA formation was assayed by thiobarbituric reactive substance formation method [35] . The TBA-MDA chromophore was taken as an index of LPO, which gives a pink colour, which was measured at 535 nm and the results were expressed in nanomoles of MDA per mg protein.…”