Hypoglycaemic Effect of Glibenclamide: A Critical Study on the Basis of Creatinine and Lipid Peroxidation Status of Streptozotocin-induced Diabetic Rat

Abstract

Seena, et al.: A Critical Study on Hypoglycaemic Effect of GlibenclamideThe present study is aimed to evaluate the hypoglycaemic effect of glibenclamide monotherapy on streptozotocin-induced diabetic rats and therapeutic impact of this agent on various organs by measuring the oxidative stress biomarker, lipid peroxidation and creatinine after a specific period of oral treatment. Eighteen male Wistar rats were taken of which twelve were randomly selected and diabetes was induced using streptozotocin (40 mg/kg) intraperitoneally, while 6 rats were vehicle injected. After inducing diabetes, the animals were grouped into healthy normal, diabetic control and diabetic test groups. The diabetic test rats were treated with glibenclamide (10 mg/kg) orally for 10 days continuously. On the 30th day of the experiment, all the animals were sacrificed and their blood and a portion of both the liver and pancreas were taken for biochemical analysis. The experimental parameters such as body weight, fasting blood glucose level, serum creatinine level and tissue lipid peroxidation status using malondialdehyde assay were investigated. All the results of the experimental data were analysed statistically using one-way analysis of variance using the SPSS 16. The current study reveals that glibenclamide treatment has a significant role in lowering the blood glucose but at the same time it increased the oxidative stress, which was reflected in the elevated malondialdehyde activity of both the hepatic and pancreatic tissues.

Long-Term Oral Treatment with Non-Hypoglycemic Dose of Glibenclamide Reduces Diabetic Retinopathy Damage in the Goto-KakizakiRat Model

“…In diabetic animals, we expect the intraocular biodisponibility to be higher, due to the breakdown of blood retinal barrier. It should be noted that the doses used to lower blood glucose in the GK rat (1 to 50 mg/kg) are higher than in humans [ 47 , 48 , 49 , 50 ].…”

In vivo Assessment of Combined Effects of Glibenclamide and Losartan in Diabetic Rats

“…In contrast, GLI, as a K ATP channel blocker, abolished sodium hydrosulfide neuroprotection against oxidative stress in one study [24]. Moreover, GLI monotherapy had unfavorable effects on lipid peroxidation in the liver and pancreatic tissues of diabetic animals, suggesting the need for combination therapy to restore the antioxidant potential of the drug [25]. Interestingly, Chatuphonprasert et al [26] reported that GLI co-therapy with berberine did not provide additional value for either medication, which indicates that the therapeutic value may be restricted to single, but not combined, treatments.…”

Phenolic profiles, antihyperglycemic, antihyperlipidemic, and antioxidant properties of pomegranate (Punica granatum) peel extract

“…Drugs are more costly and cause several side effects such as disorder in liver and kidney functions and disturbance in hematological properties. Khalil (2004), Salwe, Sachdev, Bahurupi, and Kumarappan (2015), and Pandarekandy, Sreejesh, Harikumaran Thampi, and Sreekumaran (2017) revealed that those drugs could be replaced by natural bioproducts, which are more safe and cheaper.…”