Antioxidant and Cytotoxic Properties of Soft Coral Nepthea sp.

Sadarun, Baru; Rahmatika, Nur Syifa; Yodha, Agung Wibawa Mahatva; Fristiohady, Adryan; Sundowo, Andini; Baharum, Syarul Nataqain; Sahidin, Idin

doi:10.14710/ik.ijms.27.1.29-36

Cited by 7 publications

(11 citation statements)

References 23 publications

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“…Based on the cytotoxicity level (Sadarun et al, 2022), the extract is categorized as very active (IC 50 < 10 mg.ml −1 ), active (10-100 mg.ml −1 ), and moderately active (100-500 mg.ml −1 ). Thus, the ethyl acetate extract and fraction E were categorized as moderately active, while fraction C was categorized as active against MCF-7 breast cancer cells lines.…”

Section: Resultsmentioning

confidence: 99%

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Phytochemical screening and antioxidant and cytotoxic activities of ethyl acetate subfractions of soft coral Nepthea sp. growing in Southeast Sulawesi

Sahidin¹,

Sadrun²,

Rahmatika³

et al. 2022

J App Pharm Sc

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Nepthea sp. is a soft coral that grows abundantly in the seas of Southeast Sulawesi, Indonesia. However, there is no information available regarding its pharmacological or chemical characteristics. As a result, the goal of this research was to uncover the chemical profile of the Nepthea sp. ethyl acetate subfractions, as well as their antioxidant and anticancer potential. The sample was extracted with ethyl acetate and then fractionated using vacuum liquid chromatography with Si-gel as an adsorbent and a chosen solvent as an eluent. Phytochemical tests, Liquid Chromatography-Mass Spectroscopy/Mass Spectroscopy (LC-MS/MS), and total phenolic content were used to determine the chemical content. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals were used to test the antioxidant potency, whereas MCF-7 cell lines were used in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide experiment to evaluate cytotoxicity. The fractionation of the ethyl acetate extract (160 g) produced six subfractions: Fractions A (35.2 g), B (4.3 g), C (5.9 g), D (10.7 g), E (26.5 g), and F (15.4 g). According to the DPPH and ABTS results, fraction E has the highest antioxidant potency (IC 50 = 67.39 ± 1.56 and 54.12 ± 0.95 mgl −1 , respectively), and fraction C has the highest anticancer activity (IC 50 = 72.82 ± 1.30 mgl −1 ). Fraction C components include 3-acetyl-3,4-dihydro-5,6-dimethoxy-2(1)H-benzopyrone, oxyphyllenone B, and unidentified chemicals, according to LC-MS/MS data (C 15 H 21 NO, C 21 H 33 NO 2 , C 15 H 23 NO 3 , C 15 H 21 NO 2 , C 15 H 21 NO 3 , and C 45 H 84 O 14). Rengyolester, piperolactam-C9:1(8E), valine, and unidentified chemicals (C 52 H 79 N 3 O, C 32 H 51 NO 7 ) make up fraction E. As a result, the ethyl acetate extract and its subfractions from Nepthea sp., especially fractions C and E, can be used as a source of raw materials for anticancer agents and antioxidants, respectively.

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Section: Resultsmentioning

confidence: 99%

“…Screening of secondary metabolites in all the samples was done using the Harborne methods (Sadarun et al, 2022).…”

Section: Screening Of Phytochemical Contentsmentioning

confidence: 99%

Phytochemical screening and antioxidant and cytotoxic activities of ethyl acetate subfractions of soft coral Nepthea sp. growing in Southeast Sulawesi

Sahidin¹,

Sadrun²,

Rahmatika³

et al. 2022

J App Pharm Sc

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“…Furthermore, the ethyl acetate extract was fractionated using liquid vacuum chromatography (VLC) with Si-gel adsorbent and a mixture of n-hexane: ethyl acetate and methanol as the eluent. [6].…”

Section: Soft Coral Collection Preparation and Extractionmentioning

confidence: 99%

“…To identify the secondary metabolite of the ethyl acetate fractions of the soft coral Lobophytum sp., phytochemical screening was done. The Harborne Method was used to assess the secondary metabolites, which included flavonoids, saponins, tannins/polyphenols, alkaloids, and terpenoids [6]. Additionally, utilizing this instrument's regular operating method, LC-MS/MS analysis was used to determine the precise chemical composition of Lobophytum sp.…”

Section: Phytochemical Screeningmentioning

confidence: 99%

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Antioxidant, Toxicity and Secondary Metabolites Contents of Ethylacetate Fraction from Soft Coral Lobophytum Sp. Growing in South East Sulawesi

Sahidin

Fristiohady

Sadarun

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Soft coral Lobophytum sp is widely distributed in the waters of South East Sulawesi, Indonesia. However, no research data on the chemical and medicinal aspects of this genus from this region have been published. Therefore, this article aims to describe the findings of those aspects of Lobophytum sp. from this region. Ethyl acetate was used to extract the sample, and Vacuum liquid chromatography (VLC) was used to fractionate the ethyl acetate extract. The DPPH radicals and ABTS methods were used to assess antioxidant potency, the BSLT method was used to evaluate toxicity, and the Phytochemical test and LCMSMS method were used to determine the chemical composition. The results showed that the ethyl acetate extract was produced in seven fractions namely Fraction A-G. The weight of each extract was A (12.8% w/w), B (9.7%), C (10.1%), D (2.0%), E (7.0%), F (25, 3%) and G (11.5%). Further studies were carried out on the fraction F which was the fraction with the highest weight. Based on the level of antioxidant power proposed by Blois, the antioxidant potential of fraction F is a strong category with an IC50 value (μg/mL) 99.13 (DPPH) and 79.30 (ABTS). Fraction F is also not toxic with LC50 198.21 μg/mL. Those activities were supported by the qualitative phytochemical screening that exhibited the extract contains phenolic compounds. LCMSMS data indicated that fraction F of ethyl acetate extract contains fragransol D, lirioresinol A, neociwujiaphenol, phillygenin, pluviatelol, saurufuran B, β-hydroxyisovalerylshikonin and some unidentified compounds with molecular formulas C21H26O8, C23H26O8, C19H24O6.

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Study In-vitro and in-silico of ethylacetate extract and fractions of soft coral Lobophytum sp. towards Artemia salina Brine Shrim (BSLT)

Sahidin,

Fristiohady,

Wahyuni

et al. 2023

BIO Web Conf.

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The article aims to describe the findings of chemical and pharmaceutical aspects of Lobophytum sp. from Southeast Sulawesi, Indonesia. Ethylacetate extract was fractionated by Vacuum liquid chromatography (VLC). Toxicity was evaluated by BSLT test, and the phytochemical screening and LCMSMS method were used to determine the chemical composition and molecular docking for in-silico study. The results showed that the ethylacetate extract was produced seven fractions namely Fraction A-G. The weight of each fraction was A (12.8% w/w), B (9.7%), C (10.1%), D (2.0%), E (7.0%), F (25, 3%) and G (11.5%). The toxicity potency of Fraction B is the most toxic with LC50 (mg/L) 26.70 ± 0.58. LCMSMS data indicated that the fraction B contains 19L-glukocyl-14-deoxy-11,12-didehydrographoside, 3-isoazmalicine, abietraticine, arachidonic acid, neociwujiaphenol, oxyphyliacinol, saurufuran B and some unidentified compounds with molecular formulas C37H46O7, C35H44O5, and C20H26O2. Based on computational simulations, Ar-Abietatriene and 3-Isoajmalicine have the potential to inhibit CDK-6. These compounds hinder the progression of the cell cycle and the proliferation of cancer cells by forming molecular interactions with residues Ile19, Val27, Ala41, Val77, Phe98, Val101, Leu152, and Ala162. This suggests their potential as anticancer agents. Thus, Fraction B can be continued for the anticancer evaluation.

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Antioxidant and Cytotoxic Properties of Soft Coral Nepthea sp.

Cited by 7 publications

References 23 publications

Phytochemical screening and antioxidant and cytotoxic activities of ethyl acetate subfractions of soft coral Nepthea sp. growing in Southeast Sulawesi

Phytochemical screening and antioxidant and cytotoxic activities of ethyl acetate subfractions of soft coral Nepthea sp. growing in Southeast Sulawesi

Antioxidant, Toxicity and Secondary Metabolites Contents of Ethylacetate Fraction from Soft Coral Lobophytum Sp. Growing in South East Sulawesi

Study In-vitro and in-silico of ethylacetate extract and fractions of soft coral Lobophytum sp. towards Artemia salina Brine Shrim (BSLT)

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