Antioxidant and Antimutagenic Properties of <i>Hibiscus</i> Tiliaceus L. Methanolic Extract

Rosa, Renato Moreira; Melecchi, Maria Inês Soares; Halmenschlager, Rafael da Costa; Abad, Fernanda Contieri; Simoni, Cristina Rosat; Caramão, Elina Bastos; Henriques, João Antônio Pêgas; Saffi, Jenifer; Ramos, Ana Lı́gia Lia de Paula

doi:10.1021/jf061407b

Cited by 34 publications

(24 citation statements)

References 39 publications

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“…The plant is also introduced as a feral species in several parts of the world including South-Western Australia and in Southern Africa. It is known by many names including hau (Hawaiian), purau (Tahitian) and ''algodoeiro da praia" (Brazil) (Rosa et al, 2006). The flowers are widely used in birth control in Asian and African countries (Brondegaard, 1973).…”

Section: Hibiscus Tiliaceusmentioning

confidence: 99%

Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus

et al. 2010

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Section: Hibiscus Tiliaceusmentioning

confidence: 99%

Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus

et al. 2010

Self Cite

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“…of induced colonies in the sample/no. of spontaneous in the negative control); (b) a significant response for analysis of variance (p ≤ 0.05) was found; and (c) a reproducible positive doseresponse (p ≤ 0.01) was detected (Myers et al 1991;Rosa et al 2006;Mortelmans and Zeiger 2000).…”

Section: Mutagenicity Assaymentioning

confidence: 99%

Novel antimicrobial peptide specifically active against Porphyromonas gingivalis

et al. 2015

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Porphyromonas gingivalis, the major etiologic agent of chronic periodontitis, produces a broad spectrum of virulence factors, including outer membrane vesicles, lipopolysaccharides, hemolysins and proteinases. Antimicrobial peptides (AMPs) including bacteriocins have been found to inhibit the growth of P. gingivalis; however, these peptides are relatively large molecules. Hence, it is difficult to synthesize them by a scale-up production. Therefore, this study aimed to synthesize a shorter AMP that was still active against P. gingivalis. A peptide that contained three cationic amino acids (Arg, His and Lys), two anionic amino acids (Glu and Asp), hydrophobic amino acids residues (Leu, Ile, Val, Ala and Pro) and hydrophilic residues (Ser and Gly) was obtained and named Pep-7. Its bioactivity and stability were tested after various treatments. The mechanism of action of Pep-7 and its toxicity to human red blood cells were investigated. The Pep-7 inhibited two pathogenic P. gingivalis ATCC 33277 and P. gingivalis ATCC 53978 (wp50) strains at a minimum bactericidal concentration (MBC) of 1.7 µM, but was ineffective against other oral microorganisms (P. intermedia, Tannerella forsythensis, Streptococcus salivarius and Streptococcus sanguinis). From transmission electron microscopy studies, Pep-7 caused pore formation at the poles of the cytoplasmic membranes of P. gingivalis. A concentration of Pep-7 at four times that of its MBC induced some hemolysis but only at 0.3%. The Pep-7 was heat stable under pressure (autoclave at 110 and 121 °C) and possessed activity over a pH range of 6.8-8.5. It was not toxic to periodontal cells over a range of 70.8-4.4 μM and did not induce toxic pro-inflammatory cytokines. The Pep-7 showed selective activity against Porphyromonas sp. by altering the permeability barriers of P. gingivalis. The Pep-7 was not mutagenic in vitro. This work highlighted the potential for the use of this synthetic Pep-7 against P. gingivalis.

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“…Cells growth was done in complete liquid medium (YEL) containing 1% yeast extract, 2% bactopepto- [31]. Suspended cells were seeded from the center to the edge of a sterile, solidified YEPD media containing petridish in a continuous cycle, for both sides of the plate in the center containing a sterile filter paper disk.…”

Section: Culture Mediamentioning

confidence: 99%

Net effects of cashew nuts in Saccharomyces cerevisiae front to damage induced by hydrogen peroxide

Leite¹,

Gomes²,

Oliveira³

et al. 2016

Int Arch Med

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Cellular injury associated with oxidative stress has been related in the etiology and progression of many diseases, including neurodegenerative diseases and cancer. Shell of cashew nut releases the alkylphenols oil, known as cashew nut shell liquid (CNSL), which can be characterized according to the method of production such as technical (tCNSL) and in natural (iCNSL) types. This study aimed to characterize the chemical constituents in both liquids by gas chromatography coupled to mass spectrometry (GC-MS), and to evaluate the net effect of them in proficient (SODWT) and deficient (Sod1∆, Sod2∆, Cat1∆, Sod1∆Sod2∆, Sod1∆Cat1∆) Saccharomyces cerevisiae oxidative damaged induced by hydrogen peroxide (H 2 O 2 (10 mM); stressor) at concentrations of 17.37, 34.7 and 69.5 µg/ml in pre-, co-and post-treatment. Both iCNSL and tCNSL produced no oxidizing effect to the tested yeast strains, otherwise they protected against damage induced by stressor (STR). However, tCNSL was found more prominent than the iCNSL in restorative activity. In conclusion, CNSLs (iCNSL/tCNSL) exhibited excellent protective, antioxidant and repair capabilities compared to the damage induced by STR in S. cerevisiae cells, thus may be a suggestion for its biotechnological production especially for pharmaceutical account.

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Antioxidant and Antimutagenic Properties of Hibiscus Tiliaceus L. Methanolic Extract

Cited by 34 publications

References 39 publications

Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus

Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus

Novel antimicrobial peptide specifically active against Porphyromonas gingivalis

Net effects of cashew nuts in Saccharomyces cerevisiae front to damage induced by hydrogen peroxide

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