Antioxidant and anticancer activities of Annona muricata L. and Antidesma bunius L. leaves, and molecular networking analysis using LC-MS/MS metabolomics

Peña, Aya Gabriella; Alvarez, Michael Russelle; Delica, Kimberly; Moreno, Patrick Gabriel; Abogado, Rowell; Grijaldo, Sheryl Joyce; Salac, Edcyl Lee O.; Deniega, Froila Marie; Basingan, Manolo; Ravidas, Cherry Mae; Heralde, Francisco M.; Completo, Gladys C.; Padolina, Isagani; Nacario, Ruel C.

doi:10.1016/j.sajb.2022.06.019

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…First, we created a database of all known and reported compounds found in select plant species that were reported to have cytotoxic activities: Mangifera indica , Artocarpus heterophyllus , Annona muricata , Lansium domesticum , Manilkarazapota , Antidesma bunius . Using a LC-MS/MS metabolomics workflow, it was previously reported that leaf extract fractions from these plant species contained characteristic phytochemicals (phenolics and flavonoids) that exhibited cytotoxic activities. − …”

Section: Discussionmentioning

confidence: 99%

“…Six Philippine fruit trees, namely Lansium domesticum (langsat/lanzones), Mangifera indica (mango), Artocarpus heterophyllus (jackfruit/langka), Antidesma bunius (bignay), Manilkara zapota (sapodilla/chico), and Annona muricata (soursop/guyabano), were selected as possible sources of phytochemicals against lung cancer. We previously reported that leaf extract fractions from these plants have high cytotoxic activity against lung cancer cells. − An in-house database composed of 226 natural product compounds previously isolated from L. domesticum , − M. indica , − A. muricata , A. bunius , , M. zapota , and A. heterophyllus was prepared based on extensive literature search using the PubMed NCBI database (). This was also supplemented with chemical information retrieved from PubChem NCBI database ().…”

Section: Methodsmentioning

confidence: 99%

“…We previously reported that leaf extract fractions from these plants have high cytotoxic activity against lung cancer cells. 13 − 16 An in-house database composed of 226 natural product compounds previously isolated from L. domesticum , 17 − 22 M. indica , 23 − 26 A. muricata , 27 A. bunius , 15 , 28 M. zapota , 29 and A. heterophyllus was prepared based on extensive literature search using the PubMed NCBI database ( ). This was also supplemented with chemical information retrieved from PubChem NCBI database ( ).…”

Section: Methodsmentioning

confidence: 99%

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Integrating Computational Methods in Network Pharmacology and In Silico Screening to Uncover Multi-targeting Phytochemicals against Aberrant Protein Glycosylation in Lung Cancer

et al. 2023

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Glycoproteins are an underexploited drug target for cancer therapeutics. In this work, we integrated computational methods in network pharmacology and in silico docking approaches to identify phytochemical compounds that could potentially interact with several cancer-associated glycoproteins. We first created a database of phytochemicals from selected plant species, Manilkara zapota (sapodilla/chico), Mangifera indica (mango), Annona muricata (soursop/guyabano), Artocarpus heterophyllus (jackfruit/langka), Lansium domesticum (langsat/lanzones), and Antidesma bunius (bignay), and performed pharmacokinetic analysis to determine their drug-likeness properties. We then constructed a phytochemical–glycoprotein interaction network and characterized the degree of interactions between the phytochemical compounds and with cancer-associated glycoproteins and other glycosylation-related proteins. We found a high degree of interactions from α-pinene (Mangifera indica), cyanomaclurin (Artocarpus heterophyllus), genistein (Annona muricata), kaempferol (Annona muricata and Antidesma bunius), norartocarpetin (Artocarpus heterophyllus), quercetin (Annona muricata, Antidesma bunius, Manilkara zapota, Mangifera indica), rutin (Annona muricata, Antidesma bunius, Lansium domesticum), and ellagic acid (Antidesma bunius and Mangifera indica). Subsequent docking analysis confirmed that these compounds could potentially bind to EGFR, AKT1, KDR, MMP2, MMP9, ERBB2, IGF1R, MTOR, and HRAS proteins, which are known cancer biomarkers. In vitro cytotoxicity assays of the plant extracts showed that the n-hexane, ethyl acetate, and methanol leaf extracts from A. muricata, L. domesticum and M. indica gave the highest growth inhibitory activity against A549 lung cancer cells. These may help further explain the reported cytotoxic activities of select compounds from these plant species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Integrating Computational Methods in Network Pharmacology and In Silico Screening to Uncover Multi-targeting Phytochemicals against Aberrant Protein Glycosylation in Lung Cancer

et al. 2023

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“…The homogenized dried leaves were subjected to sequential extraction 8 using hexane, ethyl acetate, and methanol as solvents. Half of the dried leaves of L. domesticum was initially soaked in hexane (1:10 w/v) and extracted three times for a total of 24 h, in 4 h intervals (first and second extraction) and 16 h for the last extraction, at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomics reveals anticancer compounds from Lansium domesticum against NSCLC cells target mitochondrial processes

Alvarez

Juan

Zhou

et al. 2023

Cell Biochemistry & Function

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Lansium domesticum is identified as a potential source of anticancer compounds. However, there are minimal studies on its anti-lung cancer properties as well as its mechanism of action. Here, we show the specificity of lanzones hexane (LH) leaf extracts to non-small cell lung cancer cells (A549) compared to normal lung fibroblast cells (CCD19-Lu) and normal epithelial prostate cells (PNT2). Subsequent bioassay-guided fractionation of the hexane leaf extracts identified two bioactive fractions with IC 50 values of 2.694 μg/ml (LH6-6) and 2.883 μg/ml (LH7-6). LH 6-6 treatment (1 μg/ml concentration) also showed a significantly reduced migration potential of A549 relative to the control. Thirty-one phytocompounds were isolated and identified using gas chromatography-mass spectrometric (MS) analysis and were then subjected to network pharmacology analysis to assess its effects on lung cancer target proteins. Using liquid chromatography-tandem mass spectrometry proteomics experiments, we were able to show that these compounds cause cytotoxic effects through targeting mitochondrial processes in A549 lung cancer cells.

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“…The total flavonoids in bignay fruit are higher than AVG. In a study conducted by [19] total flavonoid bignay 120.39 mg/100 g FW. The flavonoid content turned into 138.60-289.60 mg catechin (CE)/100 g DW [1].…”

Section: A Anthocyaninsmentioning

confidence: 99%

Effect of AVG on Anthocyanin and Antioxidant Activity of Bignay Fruit Juice

Suriati¹,

Hidalgo²,

Mangku³

et al. 2023

Int. J. Adv. Sci. Eng. Inf. Technol.

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Consumption of fruit juices product containing various bioactive compounds that are good for health is a current trend. Bignay fruit is a tropical fruit that contains high anthocyanin and can increase its antioxidant ability by adding aloe vera gel (AVG). Bignay fruit also contains organic acids, namely gallic, ferulic, and ellagic acids. AVG is associated with many transparent or colorless polysaccharides and contains functional bioactive chemical compounds. This research aimed to evaluate the effect of AVG concentration on the anthocyanin and antioxidant activity of bignay fruit juice. This research design used a completely randomized design of one factor, namely the concentration of AVG. The formulation of bignay fruit juice with the addition of AVG at concentrations of 25%, 50%, and 75% at a temperature of (6Â±1) Â°C was carried out. The observed variables included total anthocyanins, total flavonoids, vitamin C, antioxidant activity, viscosity, and acidity. All experimental units were repeated three times. Periodic observations of bignay fruit juice were carried out on storage 0, 3, 6, 9, 12, and 15 days. The AVG concentration significantly affected the total anthocyanin, antioxidant activity, and viscosity of bignay fruit juice. The best formula for bignay fruit juice is the addition of AVG with a concentration of 25%. The bignay fruit juice notes a large number of functional compounds until the ninth day of storage. Bignay fruit juice can be a recommendation for health-conscious consumers to fulfill their functional drink desires.

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Antioxidant and anticancer activities of Annona muricata L. and Antidesma bunius L. leaves, and molecular networking analysis using LC-MS/MS metabolomics

Cited by 8 publications

References 21 publications

Integrating Computational Methods in Network Pharmacology and In Silico Screening to Uncover Multi-targeting Phytochemicals against Aberrant Protein Glycosylation in Lung Cancer

Integrating Computational Methods in Network Pharmacology and In Silico Screening to Uncover Multi-targeting Phytochemicals against Aberrant Protein Glycosylation in Lung Cancer

Comparative proteomics reveals anticancer compounds from Lansium domesticum against NSCLC cells target mitochondrial processes

Effect of AVG on Anthocyanin and Antioxidant Activity of Bignay Fruit Juice

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