Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315

Li, Shengjie; Huang, Renhui; Shah, Nagendra P.; Tao, Xueying; Xiong, Yonghua; Wei, Hua

doi:10.3168/jds.2014-7912

Cited by 167 publications

(100 citation statements)

References 32 publications

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“…A moderate antioxidant activity of methanol extract of exopolysaccharide from Cyanobacterium Arthrospira platensis using the Trolox Equivalent Antioxidant Activity assay was determined by Challouf et al (2011) [11]. A strong scavenging ability of exopolysaccharide from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315 was observed against DPPH and superoxide radicals at high concentration [15]. The EPS produced by Lactobacillus plantarum YW32 at a dose of 5mg/ml had strong scavenging abilities toward hydroxyl and superoxide radicals [17].…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

Ghalem¹

2017

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Abstract:The in vitro antimicrobial and antioxidant activities of the exopolysaccharide (EPS) extracted from yoghurt starter were investigated. Antimicrobial activity tests were carried out using disc diffusion methods with Escherichia coli, Staphylococcus aureus and Streptococcus D, Proteus spp and the yeast of Candida albicans. The antioxidant properties were evaluated using the test of DPPH free radical trapping. The results show that the EPS has weak antimicrobial activity against the tested strains with inhibitions zones ranging from 9 to 13 mm. EPS could reduce the free radical (DPPH) to diphenylpicrylhydrazine a yellow-colored at 24.25% showing antioxidant activity less than that of ascorbic acid which was 69.79%.

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Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that microbial polysaccharides act as an effective antioxidant [ 25,15,17,24], but very limited information is available on the antioxidant mechanisms of the exopolysaccharides action at molecular level [6,26].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

Ghalem¹

2017

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“…9, No. 2; other studies against human bacteria (Li et al, 2014;Roselló et al, 2013). Therefore, it is suggested that the EPS could have acted against the pathogen through the activation of resistance mechanisms in tomato plants, even with the plant protection occurring in a short interval between EPS application and the inoculation of the plants (3 days), and the lacking of bacterial spot control when applied 7 days before the treatment.…”

Section: Characmentioning

confidence: 99%

Lactobacillus plantarum Exopolysaccharides Induce Resistance against Tomato Bacterial Spot

Blainski¹,

Neto²,

Luiz³

et al. 2017

JAS

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Lactic acid bacteria produce several exopolysaccharides (EPS) that may have antimicrobial action and/or induce defense responses in plants. This work aims to evaluate the potential of EPS produced by Lactobacillus plantarum in the protection of tomato plants against bacterial spot caused by Xanthomonas gardneri, as well as to predict the possible mechanisms of action. The EPS were characterized through FTIR and applied at 0; 0.5; 1.5 and 3.0 mg mL -1 in tomato plants with five expanded leaves, followed by the pathogen inoculation after 3 or 7 days. Antimicrobial activity of the biopolymer (1.5 or 10.0 mg mL -1 ) was evaluated in bioassay when EPS was incorporated into culture medium or embedded in antibiogram disk. The defense mechanisms i.e., total phenolic compounds and flavonoids content, phenylalanine ammonia-lyase (PAL), glutathione reductase (GR) and lipoxygenase (LOX) activities, were measured in tomato plants treated with EPS (1.5 mg mL -1 ), inoculated or not with X. gardneri. EPS reduced bacterial spot symptoms by up to 72.0% compared to the control. There were no direct effects of EPS on the in vitro growth of X. gardneri. The spectrophotometric profile, ascorbic and ellagic acid concentrations were change in tomato plants after EPS application, in plants challenged with the pathogen. Increases in PAL, GR and LOX activities were observed in plants treated with EPS. Thus, the application of L. plantarum exopolysaccharides can be considered as an effective alternative for controlling bacterial spot in tomato plants. This paper also discusses how these exopolysaccharides reduced the severity of the disease.

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“…Study by Li., et al [19] reported that exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315 exhibited an- The efficiency to produce EPS by bacterial strain is also crucial, if the EPS is to be produced at industrial scale due to its significant use. Our results were found comparable and better as compared to earlier report which suggested to recover bacterial EPS from basal medium was 90.66 ± 16.8 mg/100 ml of dry weight [23], whereas the present study recovered 259 and 312 mg/100 ml of EPS from the isolates KPEP3 and KPEP4 respectively indicating the potent EPS producers have been isolated in the present study.…”

Section: Phenotypic and Biochemical Characterization Of The Epsproducmentioning

confidence: 99%

Exopolysaccharide (EPS) Producing Isolates from Sugarcane Field Soil and Antibacterial Activity of Extracted EPSs

Patel¹,

Shah²,

Dwivedi³

2018

acta scient microb

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Keywords: Exopolysaccharides (EPSs); Bacterial EPS; Antibacterial Activity Microbial exopolysaccharides (EPSs) are reported to possess unique physiological activities including anti-microbial, anti-tumor, and anti-inflammatory. The antimicrobial activities of EPS could be used as a potential source for the development of antimicrobial drugs and may have various industrial, pharmaceutical and medical applications. The present study was aimed to isolate efficient EPS producing bacteria from sugarcane field soil and to evaluate the antibacterial activity of EPSs. Total 9 EPS producing isolates were obtained out of which, KPEP3 and KPEP4 were found to be higher producers of EPS. The isolates were able to grow at different pH but EPS production was higher at pH 6. The maximum carbohydrate content was found in KPEP3 and minimum protein content was found in KPEP4 suggestive of their probable use in medicine due to less immunogenicity. The evaluation of antibacterial activity of EPS suggested that all 4 test microorganisms (E. coli, B. cereus, B. subtilis and V. cholerae) showed sensitivity against EPSs but the sensitivity varied from one microorganism to another. The KPEP3 and KPEP4 EPSs were found to exert higher antibacterial activity on diverse test organisms. EPSs concentration of 7.5 mg/ml was found to be effective for antibacterial activity. The MIC values of EPSs suggested that they can be used at lower concentration to inhibit test organisms. The biochemical characterization indicated that these isolates may belong to genera: Bacillus and Pseudomonas. Further research is necessary to elucidate the composition and efficacy of the EPSs by using in vitro and in vivo animal model studies. AbbreviationsExopolysaccharides (

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Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315

Cited by 167 publications

References 32 publications

Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

Antioxidant and Antimicrobial Activities of Exopolysaccharides from Yoghurt Starter

Lactobacillus plantarum Exopolysaccharides Induce Resistance against Tomato Bacterial Spot

Exopolysaccharide (EPS) Producing Isolates from Sugarcane Field Soil and Antibacterial Activity of Extracted EPSs

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