ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF NONPOLAR TO POLAR SOLVENT EXTRACTS OF Sarang Banua (Clerodenrum fragrans Vent Willd) LEAVES

Abstract

Sarangbanua plant, known as Clerodendrumfragrans Vent Willd, is a division of the Verbanaceae family found in Simalungun district of Indonesia, which is traditionally used as medicinal plants. The antioxidant and antibacterial activities of the C. fragrans leave extract was prepared by dissolving it in non-polar to polar solvents and treated by DPPH (1,1diphenyl-2-picrylhydrazin) and diffusion methods. The results showed that the ethanol (polar) extract of the C. fragrans leaves had the highest antioxidant activity than ethyl acetate (semi-polar) and n-hexane (non-polar) extracts, which had IC 50 values of 22.37 ppm, 27.26 ppm, and 88.77 ppm respectively. Vitamin C as a positive controlled with the IC 50 value of 20.18 ppm. Furthermore, the ethyl acetate (semi-polar) extract of the C. fragrans leaves had the greatest inhibition value on Gram-positive bacteria of S. aureus (90.0%)with inhibitory zones of 11.25 ± 0.2 mm, meanwhile the n-hexane (non-polar) extract had the greatest inhibition against Gram-negative bacteria of the E.coli(77.21%)with inhibitory zones of 10.5±0.3 mm.

Antioxidant activity test of ethyl acetate total extract from Batak leeks (Allium chinense G. Don)

“…When the electrons become paired, the absorbance decreased stoichiometrically according to the number of electrons taken. The presence of antioxidant compounds could change the color of DPPH solution from purple to yellow (Dehpour et al 2009;Febrina et al 2017;Simorangkir et al 2019) as shown in Fig 3. From the data, it was concluded that the ethyl acetate extract of batak leeks had strong antioxidant activity of 52.39 mg / L. According to Pokorny et al (2001) IC 50 value was strong if IC 50 between 50-100 mg / L.…”

Determination antioxidant activities methanol extracts of bangun-bangun (Coleus amboinicus L.) Leaves with DPPH method

“…The higher the content of the active compound in natural extracts, the greater the antioxidant activity. The value of the ability to inhibit free radicals from DPPH and measurement of the antioxidant activity of ascorbic acid (vitamin C) is shown in Table 3 and Fig 2. Based on data from Table 3 and Fig 2, IC50 value of ascorbic acid is 4.18 and belongs to the very strong category as an antioxidant (Situmeang et al 2016;Marjoni and Zulfisa, 2017;Simorangkir et al 2019). Ascorbic acid activity is still stronger than the methanol extract of Coleus amboinicus leaves.…”

Antibacterial activity test of Simargaolgaol (Aglaonema modestum Schott ex Engl) leaves extract against Escherichia coli and Salmonella typhi bacteria

“…Escherichia coli (ATCC 25922) and Salmonella typhi (ATCC 14028) bacteria were cultured in sterilized petri dishes, then incubated for 24 hours at 37 o C (Simorangkir et al 2019b).…”