Antioxidant and Antibacterial Activities for Several Phenolic Compounds in Selected Personal Care Products

Abstract

The radical scavenging activity of phenolic compounds such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methylcinnamat (OMC) as UVB-filter in selected personal care products such as sunscreen cream, milk lotion, hair oil and hair gel were evaluated. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical was used as reagent and antioxidant activity expressed as decrease in the percentage of DPPH. The antibacterial activities of BHA, BHT, OMC and mixture were assessed using two techniques of agar plate diffusion method. The mixture of BHA, BHT and OMC showed the highest percentage of DPPH• inhibition (95.2%), followed by BHA (94.8%), BHT (93.9%) and OMC (9.0%). DPPH• inhibition for crude extracts of selected personal care products were evaluated. The percentage of DPPH• inhibition for sunscreen cream, milk lotion, hair gel and hair oil were 15.3%, 23.5%, 7.7% and 12.7%, respectively. While IC50 of BHA, BHT and mixture were observed at 29.83, 44.16, 32.51 mg/L, respectively. The MIC values for BHA, BHT, OMC and mixture against E. coli using paper-disk diffusion technique were 1.5, 15, 200, 1.5 μg/disk, respectively. While, the MIC values obtained using hole-punch technique were 100, 200, not active, 70 μg/hole for BHA, BHT, OMC and mixture, respectively. In addition, the MIC values obtained by paper-disk diffusion technique for BHA, BHT, OMC, and mixture against S. aureus were 15, 70, not active, 10 μg/disk respectively. On the other hand, MIC values obtained using hole-punch technique were 70, 200, not active, 60 μg/hole for BHA, BHT, OMC and mixture, respectively.