Antioxidant and antiapoptotic effects of green tea polyphenols against azathioprine-induced liver injury in rats

El-Beshbishy, Hesham A.; Tork, Ola M.; El-Bab, Mohamed Fath; Autifi, Mohamed A.

doi:10.1016/j.pathophys.2010.08.002

Cited by 59 publications

(23 citation statements)

References 37 publications

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“…[4] In addition, disorganization of the liver architecture with multiple focal areas of necrosis in AZA-treated mice and other small hepatocytes with deeply stained acidophilic cytoplasm and dark nuclei were reported. [57] Furthermore degenerated mitochondria, dilated cisterns of rough endoplasmic reticulum and multiple lipid droplets were noticed. A study reported that AZA-induced cell death (apoptosis), hydropic degeneration, portal fibrosis, and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Preventive role of chamomile flowers and fennel seeds extracts against liver injury and oxidative stress induced by an immunosuppressant drug in rats

Mannaa¹,

Ibrahim²,

Ibrahim³

et al. 2015

Hepatoma Res

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Aim: The present study was conducted to investigate the protective effect of chamomile fl owers methanolic extract (CFME) and fennel seeds methanolic extract (FSME) on azathioprine (AZA), an immunosuppressant drug, which induced a liver injury and oxidative stress in rats. Methods: Rats were divided into 6 groups (8 rats each) and treated orally for 28 consecutive days as follows. Group 1: rats were given normal saline and used as controls; group 2: rats treated with CFME (200 mg/kg); group 3: rats treated with FSME (200 mg/kg); group 4: rats treated with AZA (25 mg/kg); and groups 5 and 6: rats treated with CFME (200 mg/kg) or FSME (200 mg/kg) 15 min prior to AZA (25 mg/kg) treatment. At the end of experimental period, blood and liver samples were collected from all groups for biochemical analysis and histological examination. Results: The obtained data revealed that AZA-induced hepatic injury in the rats as evidenced by the signifi cant increase in serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, cholesterol, and direct bilirubin as well as hepatic malondialdehyde level accompanied with signifi cant decrease in reduced glutathione content and total antioxidant capacity in the liver. Moreover, body weight gain showed the signifi cant decrease and relative liver weight showed the signifi cant increase on AZA treatment. The sequential signifi cant changes in biochemical parameters were accompanied by severe histological changes in the liver tissue, including hepatocytes disorganization with pyknotic nuclei, fatty degeneration, congestion, fi brosis, and bile duct necrosis around the portal tract. The areas of hemorrhages in blood vessels and in between hepatocytes were also seen. However, the results showed the potential hepatoprotective effects of CFME and FSME against AZA-induced liver injury and oxidative stress. They succeeded in restoring the biochemical parameters and improving the histological picture of the liver. This improvement was more pronounced in the rats pretreated with FSME. Conclusion: It could be concluded that CFME and FSME have hepatoprotective potentials against AZA probably due to their antioxidant properties and radical scavenging activity.Key words: Azathioprine; chamomile fl owers; fennel seeds; liver; oxidative stress Preventive role of chamomile fl owers and fennel seeds Preventive role of chamomile fl owers and fennel seeds extracts against liver injury and oxidative stress induced by an extracts against liver injury and oxidative stress induced by an immunosuppressant drug in rats immunosuppressant drug in rats This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Section: Discussionmentioning

confidence: 99%

Preventive role of chamomile flowers and fennel seeds extracts against liver injury and oxidative stress induced by an immunosuppressant drug in rats

Mannaa¹,

Ibrahim²,

Ibrahim³

et al. 2015

Hepatoma Res

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“…Pretreatment with GT and WT for 35 days tended to increase the activity of SOD and CAT in the lungs and liver. Administration of GT extract to rats intoxicated with azathioprine resulted in a significant increase in CAT activity (45). Tea polyphenols have been shown to increase the activity of some detoxifying enzymes, such as glutathione peroxidase, GR, glutathione-S-transferase, CAT, and quinone reductase, in the small intestine, liver, and lungs (9).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Green and White Tea Against Benzo(a)pyrene Induced Oxidative Stress and DNA Damage in Murine Model

Kumar

Sharma

Sehgal

et al. 2012

Nutrition and Cancer

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In the current investigation, the ameliorative effect of green tea (GT) and white tea (WT) against benzo(a)pyrene (BaP) induced oxidative stress and DNA damage has been studied in the livers and lungs of Balb/c mice. A single dose of BaP (125 mg/kg, b.w. orally) increased the levels of lipid peroxidation (LPO) and decreased endogenous antioxidants such as superoxide dismutase (SOD), glutahione reductase (GR), catalase (CAT), and glutathione (GSH) significantly. Pretreatment with GT and WT for 35 days before a single dose of BaP elevated the decreased activity of GR, SOD, and CAT in liver tissue and also tended to normalize the levels of GSH and LPO in both hepatic and pulmonary tissues. The percentage of DNA in comet tail and 8-hydroxy-2'-deoxyguanosine levels reflected the decreasing pattern of DNA damage from the BaP-treated group to the groups that received pretreatment with GT and WT. Our study concludes that both GT and WT are effective in combating BaP induced oxidative insult and DNA damage. However, WT was found to be more protective than GT with respect to CAT (only in the liver), percentage of DNA in comet tail (only in the lungs), GST activity, and GSH content in both the tissues.

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“…Recent studies have recognized that tea has health-protecting benefits that include antioxidative [6–8], antimicrobial [9, 10], antiviral [11, 12], and antitumor [13–16] activities and abilities to prevent diabetes [17], obesity [18], leukemia [19], Parkinson's disease [20], and cardiovascular disease [21]. …”

Section: Introductionmentioning

confidence: 99%

The Galloyl Catechins Contributing to Main Antioxidant Capacity of Tea Made fromCamellia sinensisin China

Zhao

Liu

et al. 2014

The Scientific World Journal

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Total polyphenol content, catechins content, and antioxidant capacities of green, dark, oolong, and black teas made from Camellia sinensis in China were evaluated. The total polyphenol content of 20 samples of tea was in the range of 7.82–32.36%. Total catechins content was in the range of 4.34–24.27%. The antioxidant capacity of tea extract was determined by the oxygen radical absorbance capacity (ORAC) test and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging test. Total polyphenol content, catechins content, and antioxidant capacity decreased in the following order: green > oolong > black > dark tea. A positive correlation existed between the antioxidant capacity and total polyphenol content or catechins content (R 2 = 0.67–0.87). The antioxidant capacities of five major catechins (epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epicatechin, epigallocatechin, and catechin) were determined by online HPLC DPPH radical-scavenging; the antioxidant activity of tea was mainly attributed to the esterified catechins (EGCG or ECG).

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Antioxidant and antiapoptotic effects of green tea polyphenols against azathioprine-induced liver injury in rats

Cited by 59 publications

References 37 publications

Preventive role of chamomile flowers and fennel seeds extracts against liver injury and oxidative stress induced by an immunosuppressant drug in rats

Preventive role of chamomile flowers and fennel seeds extracts against liver injury and oxidative stress induced by an immunosuppressant drug in rats

Protective Effects of Green and White Tea Against Benzo(a)pyrene Induced Oxidative Stress and DNA Damage in Murine Model

The Galloyl Catechins Contributing to Main Antioxidant Capacity of Tea Made fromCamellia sinensisin China

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