Protective Effects of Green and White Tea Against Benzo(a)pyrene Induced Oxidative Stress and DNA Damage in Murine Model

Abstract

In the current investigation, the ameliorative effect of green tea (GT) and white tea (WT) against benzo(a)pyrene (BaP) induced oxidative stress and DNA damage has been studied in the livers and lungs of Balb/c mice. A single dose of BaP (125 mg/kg, b.w. orally) increased the levels of lipid peroxidation (LPO) and decreased endogenous antioxidants such as superoxide dismutase (SOD), glutahione reductase (GR), catalase (CAT), and glutathione (GSH) significantly. Pretreatment with GT and WT for 35 days before a single dose of BaP elevated the decreased activity of GR, SOD, and CAT in liver tissue and also tended to normalize the levels of GSH and LPO in both hepatic and pulmonary tissues. The percentage of DNA in comet tail and 8-hydroxy-2'-deoxyguanosine levels reflected the decreasing pattern of DNA damage from the BaP-treated group to the groups that received pretreatment with GT and WT. Our study concludes that both GT and WT are effective in combating BaP induced oxidative insult and DNA damage. However, WT was found to be more protective than GT with respect to CAT (only in the liver), percentage of DNA in comet tail (only in the lungs), GST activity, and GSH content in both the tissues.

Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

“…These reversible reactions generate superoxide anion radical and other reactive oxygen species (ROS) and can also directly react with nucleophilic sites on DNA. The metabolism of PAHs through peroxidative and AKR-mediated pathways is consistent with oxidative stress- associated PAH toxicity (Kumar et al ., 2012). …”

White tea (Camellia sinensis) extract reduces oxidative stress and triacylglycerols in obese mice

“…Several studies have shown the antioxidant effect of white (ALMAJANO; VILA; GINES, 2011;CALZUOLA;GIANFRANCESCHI;MARSILI, 2006;KOUTELIDAKIS et al, 2009;KUMAR et al, 2012;SEERAM et al, 2008;THRING;HILI;NAUGHTON, 2009) and green tea (KHAN et al, 2007;KUMAR et al, 2012;PANZA et al, 2008). Since obesity caused by high-fat diet is also associated to the increase in oxidative rather than reduction in hepatic production or uptake of endogenous lipids.…”

“…tea, white tea has been used to reduce oxidative stress and to treat obesity and associated diseases. However, most studies using white tea investigated its composition (ALCAZAR et al, 2007;MALINOWSKA et al, 2008;UNACHUKWU et al, 2010; VAN DER HOOFT et al, 2012), its antitumorigenic effect (ANGER; PETRE; CRANKSHAW, 2005;KUMAR et al, 2012;WANG et al, 2008), or its antioxidant action (ALMAJANO; VILA; GINES, 2011;CALZUOLA;GIANFRANCESCHI;MARSILI, 2006;KOUTELIDAKIS et al, 2009;LOPEZ;CALVO, 2011;PEREZ-JIMENEZ et al, 2011;SEERAM et al, 2008;THRING;HILI;NAUGHTON, 2009NAUGHTON, , 2011. Nonetheless, despite its popular use, there are no in vivo studies relating white tea and obesity associated with oxidative stress.…”

Effects of Benzo(a)pyrene and Ethanol on Morphology and Antioxidant Status and Transaminases in Rat Liver

“…It is a chemical carcinogen and teratogenic agent, and is produced spontaneously by environmental pollution such as traffic clustering and industrial production [11][12][13][14]. The carcinogenesis mechanism of [B(a)P] has been reported to involve DNA adduct formation during initiation stages.…”