Antioxidant and Anti-Melanogenic Activities of Hyssopus officinalis Extracts

Shin, Seo Yeon; Kim, Ha Neul; Kang, Se Won; Cho, Hong Suk; Kim, Young Ho; Park, Sun Hwa; Park, Kyung Mok

doi:10.15230/scsk.2016.42.2.195

Cited by 4 publications

(2 citation statements)

References 21 publications

(14 reference statements)

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“…Human whole blood cells (WBC) were incubated with different concentrations of essential oils (12.5, 5 and 2.5 µg/mL) or methanol extracts of hyssop herb (100, 200 and 400 µg/mL) for 30 min at 37 °C. Tested concentrations were selected on the basis of the literature data [ 53 , 54 ]. The samples were treated in parallel with a negative control of PBS for 30 min at 37 °C, as well as with a positive control of hydrogen peroxide (H 2 O 2 , 50 µM) for 20 min at 4 °C and PBS for 30 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae)

Mićović

Topalović

Živković

et al. 2021

Plants

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Hyssopus officinalis L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diode array detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxic and antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480, MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oils were rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the most abundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively). Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50 = 56.04–199.89 µg/mL, FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in human whole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selective and potent dose- and time-dependent activity against the HeLa cell line. Results of the current study demonstrated notable H. officinalis medicinal potential, which calls for further investigation.

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Section: Methodsmentioning

confidence: 99%

Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae)

Mićović

Topalović

Živković

et al. 2021

Plants

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“…However, research is ongoing to discover raw materials that exhibit better properties for preventing hyperpigmentation effects. Recently, there have been studies published on the effects of Pueraria [9], Siberian Sibiricum [10], and Hyssopus officinalis [11] on melanogenesis.…”

Section: Introductionmentioning

confidence: 99%

The Experimental Study on Inhibitory Effects of Wild Ginseng Pharmacopuncture Solution on Melanin Biosynthesis

Jo¹

2018

J Acupunct Res

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Background: The purpose of this study was to investigate the effects of wild ginseng pharmacopuncture on melanin production in B16/F10 murine melanoma cells. Methods: To determine the effect of wild ginseng pharmacopuncture solution on B16/F10 cells, cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method. To observe B16/F10 cell growth, death, and morphological changes, Trypan blue solution was used. The Hosoi method was used to investigate the effect of wild ginseng pharmacopuncture solution on melanin production. The Martinez-Esparza method was used to investigate the effect of wild ginseng pharmacopuncture solution on tyrosinase activity. To determine the pathway involved in the melanogenesis in cells exposed to wild ginseng pharmacopuncture solution, a cell-free tyrosinase was used. Results: Following treatment with 200 µL of wild ginseng solution, the cell survival rate was 76.32 ± 2.45% which significantly decreased with higher concentrations (µL) of wild ginseng (up to 200 µL). When 100 µL of wild ginseng was used, the cell survival rate was 89.95 ± 2.07%. No morphological changes or abnormalities were observed in the B16/F10 murine melanoma cells as observed in the Trypan blue test. Melanin production was significantly reduced to 72.17 ± 3.74% at 100 µL. Using 100 µL of wild ginseng solution, tyrosinase activity was significantly decreased to 80.15 ± 1.05%. Wild ginseng pharmacopuncture solution reduced melanin production both directly and indirectly. Conclusion: This study suggests that wild ginseng pharmacopuncture solution may be effective in inhibiting melanin production. Further studies are needed to determine safe and effective clinical applications.

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Evaluation of cytotoxicity, genotoxicity, and zebrafish embryo toxicity of mixtures containing Hyssopus officinalis, Morus alba, Engraulis japonicus, and 27 other extracts for cosmetic safety assessment

Kim

Choi

et al. 2021

Mol. Cell. Toxicol.

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Antioxidant and Anti-Melanogenic Activities of Hyssopus officinalis Extracts

Cited by 4 publications

References 21 publications

Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae)

Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae)

The Experimental Study on Inhibitory Effects of Wild Ginseng Pharmacopuncture Solution on Melanin Biosynthesis

Evaluation of cytotoxicity, genotoxicity, and zebrafish embryo toxicity of mixtures containing Hyssopus officinalis, Morus alba, Engraulis japonicus, and 27 other extracts for cosmetic safety assessment

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