Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes

Jin, Xi; Wang, Kai; Li, Qiang‐Qiang; Tian, Wenli; Xue, Xiao‐Feng; Wu, Liming; Hu, Fuliang

doi:10.1016/j.jff.2017.04.039

Cited by 42 publications

(33 citation statements)

References 36 publications

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“…Nrf2 is a key regulator of antioxidant signaling, and it binds to Keap1 under homeostatic condition (Zhu, Dong, Liu, Ren, & Cui, ). After the cells are exposed to ROS or other Nrf2 activators, Nrf2 separates from Keap1, enters the nucleus, and binds to AREs, subsequently, stimulating the transcription of genes, such as NQO1, GCLC and HO‐1 (Wu et al, ) that protects the cells from inflammation (Jin et al, ), oxidative stress (Xia et al, ), and neurotoxicity (Cao, Du, & Hei, ). Previous studies (Mo et al, ) showed that Nrf2 deficiency increased the accumulation of ROS in mice, and Liu et al found that saponin Ab upregulated some Nrf2‐related antioxidant enzymes to alleviate the oxidative stress (Liu, Chen, et al, ).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

et al. 2019

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Apple phlorizin has many biological activities, such as antioxidant and liver protection. The present study aimed to evaluate the roles of apple phlorizin against hydrogen peroxide (H2O2)‐induced oxidative damage in HepG2 cells. In this study, treatment with apple phlorizin (100 and 150 μg/ml) decreased the production of reactive oxygen species and alleviated apoptosis as well as DNA damage in H2O2‐induced HepG2 cells. These effects were associated with the increased activity of antioxidant enzymes, enhanced the ARE‐driven phase II antioxidant gene expression and its upstream Nrf2 protein expression, and decreased apoptosis‐related gene expression. However, the phase II antioxidant gene expression and Nrf2 protein expression upregulated by phlorizin were reversed by Nrf2 shRNA transfection. These results showed that phlorizin relieves oxidative stress, DNA damage, and apoptosis in H2O2‐induced HepG2 cells, at least partially, by regulating the expression of Nrf2 protein and apoptosis‐related genes. Practical applications Apple phlorizin is a polyphenol compound extracted from apple or apple juice. This report highlighted a protective effect of phlorizin on antioxidant stress, DNA damage, and apoptosis in H2O2‐induced HepG2 cells. These results suggested that phlorizin may be developed for functional foods.

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Section: Discussionmentioning

confidence: 99%

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

et al. 2019

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“…Chinese propolis were reported mostly to have four biological activities, antioxidant [32], antitumor [14,36], anti-inflammatory [7] and neuroprotective activities [37], which were mainly attributed to the content and the ratio of polyphenols and phenols. As the changes in the local climate and surroundings cause variations of the chemical compositions, corresponding variations of the biological activities are also expected.…”

Section: Discussionmentioning

confidence: 99%

“…Since ancient times, it has been extensively applied in folk medicines, natural food additives, functional food ingredients, and natural cosmetics [1,2]. The propolis gathered from different plant origins and geographic origins, or produced by different species of bees, shows significantly different therapeutic properties, such as antimicrobial [3], anti-tumor [4], antioxidant [5,6], anti-inflammatory [7][8][9], immunomodulatory [10], anti-diabetic [11], and anti-ulcer properties [12]. These distinct activities are mainly attributed to the complicated chemical compositions of propolis [13][14][15], 50% of the content of which originates from plant resins [16].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Reveals Discrimination of Chinese Propolis from Different Climatic Regions

Wang

Liu

Wang

et al. 2020

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The chemical profiles of propolis vary greatly due to the botanic sources and geographic origins, which limit its standardization for modern usages. Here, we proposed a reliable 1H NMR-based metabolomic approach, to discriminate the function and quality of Chinese propolis. A total 63 Chinese propolis samples from different temperate regions were collected and extracted for NMR analysis. Twenty-one compositions in ethanol extracts were assigned based on characteristic chemical shifts and previous literature reports. Significant geographic indicators were identified after the PCA and orthogonal partial least squares discriminant analysis (OPLS-DA) analysis of the obtained 1H NMR data. It was found that the composition discriminations arose from long-term acclimation of the different climates of botanic origin and caused the differences in the biological activities. This study provides us a reasonable instruction for the quality control of Chinese propolis.

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“…The pathogenesis of NAFLD remains perplexing, and the "two-hit" hypothesis has become a generally established view to guide researches [7]. The "first hit" is steatosis that mentions excessive triacylglycerol (TG) and free fatty acids (FFAs), which are generated by the disorder of hepatic lipid metabolism [8]. Mono-unsaturated fatty acids increase significantly in the FFA composition of the liver of patients with NAFLD, and oleic acid (OA) is the most important component [9].…”

Section: Introductionmentioning

confidence: 99%

“…Mono-unsaturated fatty acids increase significantly in the FFA composition of the liver of patients with NAFLD, and oleic acid (OA) is the most important component [9]. The "second hit" promotes the forming of NAFLD followed with an inflammatory response, oxidative stress, and cellular apoptosis [8]. In a current study, it is considered the multiple-hit pathogenesis of NAFLD, meaning the relationship between NAFLD and metabolic syndrome, mainly insulin resistance and oxidative stress, is interaction [3].…”

Section: Introductionmentioning

confidence: 99%

Lotus Seedpod Extracts Reduced Lipid Accumulation and Lipotoxicity in Hepatocytes

et al. 2019

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Non-alcoholic fatty liver disease (NAFLD) is closely associated with metabolic disorders, including hepatic lipid accumulation and lipotoxicity. Plant-derived polyphenols have attracted considerable attention in the prevention of NAFLD. Lotus seedpod, rich in polyphenols, is a traditional Chinese herbal medicine. Previous studies have showed that lotus seedpod possess radioprotective, antioxidant, anti-cancer, and anti-inflammatory activities. In this study, the in vitro hepatoprotective effect of lotus seedpod extract (LSE) and its main component epigallocatechin (EGC) was examined. Firstly, oleic acid (OA), an unsaturated fatty acid, was used to induce the phenotype of NAFLD in human hepatocytes, HepG2 cells. LSE dose-dependently improved the OA-induced viability loss of HepG2 cells. Non-cytotoxic concentrations of LSE or EGC abolished intracellular lipid accumulation and oxidative stress in the OA-treated cells. In addition, LSE and EGC showed a minor effect on autophagy, and potential in reducing OA-induced occurrence of apoptosis confirmed by morphological and biochemical features, including an increase in the formation of apoptotic bodies, the exposure of phosphatidylserine, and activation of caspases. Molecular data showed the anti-apoptotic effect of LSE might be mediated via downregulation of the mitochondrial pathway. Our data imply that EGC-enriched LSE potentially could be developed as an anti-NAFLD agent.

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Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes

Cited by 42 publications

References 36 publications

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

Metabolomics Reveals Discrimination of Chinese Propolis from Different Climatic Regions

Lotus Seedpod Extracts Reduced Lipid Accumulation and Lipotoxicity in Hepatocytes

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