Antioxidant and anti-inflammatory activity of peptides from foxtail millet (Setaria italica) prolamins in HaCaT cells and RAW264.7 murine macrophages

Ji, Zhongwei; Mao, Jieqi; Chen, Shuguang; Mao, Jian

doi:10.1016/j.fbio.2020.100636

Cited by 51 publications

(46 citation statements)

References 36 publications

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“…The inhibitory effect of OMEO on NO levels induced by LPS stimulation in RAW 264.7 cells was determined using Griess assay [ 8 ]. RAW 264.7 cells (3 × 10 4 cells/well) were seeded into a 96-well plate and cultured for 24 h. After then, OMEO at various concentration (12.5–100 μg/mL) were treated with LPS (1 μg/mL) for additional 24 h. One hundred μL of supernatant of the medium were added to a new 96-well plate and mixed with 100 μL of the Griess reagent (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

“…If homeostasis is broken, it can cause nitrosative damage to biological targets or organelles (e.g., proteins, lipids, DNA, and mitochondria), which can lead to cancer, stroke, or neurodegenerative diseases [ 6 , 7 ]. Nowadays, many scientific researchers are reporting studies to find natural substances with both antioxidant and anti-inflammatory activities that properly regulate ROS and RNS [ 8 , 9 ]. In addition, they have confirmed that there is some relationship between antioxidant and anti-inflammatory activities [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, many scientific researchers are reporting studies to find natural substances with both antioxidant and anti-inflammatory activities that properly regulate ROS and RNS [ 8 , 9 ]. In addition, they have confirmed that there is some relationship between antioxidant and anti-inflammatory activities [ 8 ]. This includes the fact that ROS is associated with a variety of inflammatory responses [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antioxidant, and Anti-Inflammatory Activity of Essential Oil from Omija (Schisandra chinensis (Turcz.) Baill.) Produced by Supercritical Fluid Extraction Using CO2

Lee

et al. 2021

Foods

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Schisandra chinensis (Turcz.) Baill., which is known as omija in South Korea, is mainly cultivated in East Asia. The present study aimed to investigate the chemical composition of essential oil from the omija (OMEO) fruit obtained by supercritical fluid extraction using CO2 and to confirm the antioxidant and anti-inflammatory activity of OMEO using HaCaT human keratinocyte and RAW 264.7 murine macrophages. As a result of the chemical composition analysis of OMEO using gas chromatography-mass spectrometry, a total of 41 compounds were identified. The detailed analysis results are sesquiterpenoids (16), monoterpenoids (14), ketones (4), alcohols (3), aldehydes (2), acids (1), and aromatic hydrocarbons (1). OMEO significantly reduced the increased ROS levels in HaCaT keratinocytes induced by UV-B irradiation (p < 0.05). It was confirmed that 5 compounds (α-pinene, camphene, β-myrcene, 2-nonanone, and nerolidol) present in OMEO exhibited inhibitory activity on ROS production. Furthermore, OMEO showed excellent anti-inflammatory activity in RAW 264.7 macrophages induced by lipopolysaccharide. OMEO effectively inhibited NO production (p < 0.05) by suppressing the expression of the iNOS protein. Finally, OMEO was investigated for exhibition of anti-inflammatory activity by inhibiting the activation of NF-κB pathway. Taken together, OMEO could be used as a functional food ingredient with excellent antioxidant and anti-inflammatory activity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antioxidant, and Anti-Inflammatory Activity of Essential Oil from Omija (Schisandra chinensis (Turcz.) Baill.) Produced by Supercritical Fluid Extraction Using CO2

Lee

et al. 2021

Foods

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“…WGPHs improved the total antioxidant capacity by directly scavenging free radicals, increasing the reduced form of glutathione (GSH) levels, and reducing nitric oxide (NO) overproduction. The antioxidative and anti-inflammatory effects of three peptides (MPH-A-I, IALLIPF, and PFLF) from foxtail millet, obtained by alcalase hydrolysis, was evaluated in H 2 O 2 -induced human keratinocyte HaCaT cells and RAW264.7 murine macrophages [ 179 ]. Peptides (IALLIPF and PFLF) from millet prolamins demonstrated promising antioxidant activity by effectively reducing ROS production in H 2 O 2 -induced HaCaT cells, inhibiting MDA production, and increasing GSH levels more than the peptide fraction of MPH-A-I.…”

Section: Potential Mechanisms Of Cereal Peptidic Antioxidants In Mets Preventionmentioning

confidence: 99%

Exploring Molecular Insights of Cereal Peptidic Antioxidants in Metabolic Syndrome Prevention

et al. 2021

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The prevalence of metabolic syndrome (MetS) is presently an alarming public health problem globally. Oxidative stress has been postulated to be strongly correlated with MetS, such as type 2 diabetes, obesity, hypertension, cardiovascular diseases, and certain cancers. Cereals are important staple foods which account for a huge proportion of the human diet. However, owing to recent growing demand and the search for natural antioxidants for the prevention and management of MetS, cereal peptides have gained increasing attention for developing functional ingredients or foods with substantial antioxidant properties. This review explores the current production techniques for cereal peptidic antioxidants and their potential mechanism of action in the prevention and management of MetS.

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“…This gives rise to the possibility that partial digestion of millet proteins produces short‐chain peptides, as happens with other cereals, which are absorbed directly and may have an important role in hyperglycaemia regulation 78,79 . Enzymatic‐modified foxtail protein has already shown the possibility of positive physiological effects, such as antioxidative, antimicrobial, antifungal, antihypertensive, and anti‐inflammatory behaviour (please refer to Supporting Information Table S2 for more information) 80–83 . Consumption of this specific macro‐component from foxtail millet seeds in intact or hydrolysate form can be specifically used to prevent obesity‐related diseases, such as atherosclerosis and type 2 diabetes mellitus.…”

Section: Health Benefits Of Foxtail Millet Proteinmentioning

confidence: 99%

Foxtail millet: a potential crop to meet future demand scenario for alternative sustainable protein

2020

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Foxtail millet (Setaria italica), an annual grass plant, produces seeds that possess health‐promoting properties owing to its unique protein composition containing a high content of essential amino acids. The mature foxtail seeds mainly consist of proline‐rich, alcohol‐soluble proteins (prolamin) called setarins, comprising about 60% of the total protein, with less content of disulfide cross‐linked proteins than with other cereal and millets. Protein fractionation schemes are an important tool and provide preliminary information on the nature of foxtail proteins for their applications in the field of agriculture, food pharma, and bio‐based materials. Variation in the methods of preparation can influence the composition, structure, and nutritional quality of the protein concentrate. Moreover, foxtail protein or its hydrolysate has shown several bioactive effects that can be explored further for the management of chronic diseases in humans. Additionally, owing to its low cost and excellent functional properties of flour and protein concentrate, foxtail millet can be considered as good candidate for replacing animal protein foods. Furthermore, there is huge potential for successfully developing low‐cost, protein‐rich functional food products helpful in the prevention and management of lifestyle‐related chronic diseases. © 2020 Society of Chemical Industry

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Antioxidant and anti-inflammatory activity of peptides from foxtail millet (Setaria italica) prolamins in HaCaT cells and RAW264.7 murine macrophages

Cited by 51 publications

References 36 publications

Chemical Composition, Antioxidant, and Anti-Inflammatory Activity of Essential Oil from Omija (Schisandra chinensis (Turcz.) Baill.) Produced by Supercritical Fluid Extraction Using CO2

Chemical Composition, Antioxidant, and Anti-Inflammatory Activity of Essential Oil from Omija (Schisandra chinensis (Turcz.) Baill.) Produced by Supercritical Fluid Extraction Using CO2

Exploring Molecular Insights of Cereal Peptidic Antioxidants in Metabolic Syndrome Prevention

Foxtail millet: a potential crop to meet future demand scenario for alternative sustainable protein

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