Antioxidant and Anti-hyperglycemic Activity of Polysaccharide Isolated from Dendrobium chrysotoxum Lindl

Zhao, Ya-ping; Son, Young-Ok; Kim, So-Soon; Jang, Yong-Suk; Lee, Jeong-Chae

doi:10.5483/bmbrep.2007.40.5.670

Cited by 70 publications

(49 citation statements)

References 23 publications

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“…16) In the past several decades, the polysaccharides isolated from Chinese herbs have been reported to possess activity in stimulating the function of phagocytes, inhibiting the tumor development, protecting of liver function, etc. The polysaccharides were considered to play an important role as radical scavenger for the prevention of oxidative damage to the living systems.…”

Section: Discussionmentioning

confidence: 99%

“…Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis of Hepatic Inducible Nitric Oxide Synthase (iNOS) and Cyclooxygenase-2 (COX-2) Total RNA was extracted and first strand cDNA was synthesized by reverse transcription of the total RNA using the oligo(dT) [12][13][14][15][16][17][18] primer and SuperScript™ II RNaseH-Reverse Transcriptase (Invitrogen TechLine™, Carlsbad, CA, U.S.A.). The PCR primers were designed according to the sequence in GenBank and were as follows: iNOS, sense: 5′-AAG CTG CAT GTG ACA TCG ACC CGT -3′, antisense: 5′-GCA TCT GGT AGC CAG CGT ACC GG-3′, 598 bp; COX-2, sense: 5′-ACT CAC TCA GTT TGT TGA GTC ATT C-3′, antisense: 5′-TTT GAT TAG TAC TGT AGG GTT AAT G-3′, 583 bp; β-actin, antisense: 5′-TGT CAT CTC CAG AGT GTT C-3′, sense: 5′-TGG AAT CCT GTG GCA TCC ATG AAA -3′, 348 bp.…”

Section: )mentioning

confidence: 99%

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Hepatoprotective Effects of the Polysaccharide Isolated from Tarphochlamys affinis (Acanthaceae) against CCl4-Induced Hepatic Injury

Lin

Liu

Huang

et al. 2012

Biological & Pharmaceutical Bulletin

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This study was designed to investigate the protective effects of the polysaccharide isolated from Tarphochlamys affinis (PTA) against CCl 4 -induced hepatotoxicity in rats. Liver injury was induced in rats by the administration of CCl 4 twice a week for 2 weeks. During the experiment, the model group received CCl 4 only; the treatment groups received various drugs plus CCl 4 , whereas the normal control group received an equal volume of saline. Compared with the CCl 4 group, PTA significantly decreased the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in the serum and increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) in the liver. Moreover, the content of hepatic malondialdehyde (MDA) was reduced. Histological findings also confirmed the antihepatotoxic characterisation. In addition, PTA significantly inhibited the proinflammatory mediators, such as prostaglandin E 2 (PGE 2 ), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) and myeloperoxidase (MPO). Further investigation showed that the inhibitory effect of PTA on the pro-inflammatory cytokines was associated with the down-regulation of nuclear factor-kappa B (NF-κB). In brief, our results show that the protective effect of PTA against CCl 4 -induced hepatic injury may rely on its ability to reduce oxidative stress and suppress inflammatory responses. Key words Tarphochlamys affinis (Acanthaceae); polysaccharide; hepatoprotective effect; carbon tetrachlorideIn recent years, "oxidative stress" and its adverse effects on human health has become a subject of considerable interest of research. It is a well-documented fact that exposure of organisms to the exogenous and endogenous factors generates a wide range of reactive oxygen species (ROS), resulting in the homeostatic imbalance.1,2) Production of free radicals exceeded, such as superoxide, hydroxyl radicals, hydrogen peroxide and nitric oxide (commonly designated as ROS), was found certain to play multiple important roles in tissue damage and the loss of function.3) Development in the biomedicine points to the involvement of the free radicals in many diseases. The free radicals attack the unsaturated fatty acids in the biomembranes, yielding the membrane lipid peroxidation, the decrease in membrane fluidity, the loss of enzymes and the damage to membrane proteins and leading to the cell inactivation.4) The harmful action of the free radicals can, however, be blocked by the antioxidant substances, which scavenge the free radicals and detoxify the organism. Many studies have shown that natural polysaccharide, which were found largely in fruits and vegetables, have been confirmed to play an important part as free radical scavengers in the prevention of oxidative damage in living organism and can be exploited as novel potential antioxidants, and the effects have something to do with their chemical properties and architectural characteristics.5) T...

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Section: Discussionmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Hepatoprotective Effects of the Polysaccharide Isolated from Tarphochlamys affinis (Acanthaceae) against CCl4-Induced Hepatic Injury

Lin

Liu

Huang

et al. 2012

Biological & Pharmaceutical Bulletin

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“…In this condition, use of alternative explants helps in raising innumerable genetically identical clones. So far, the regenerative potential of stemnodes has been positively tested in some orchid species (Arditti & Ernst 1993;George & Ravishankar 1997;Vij & Kaur 1998;Kanjilal et al 1999;Gangaprasad et al 2000;Pyati et al 2002;Decruse et al 2003;Basker & Narmatha Bai 2006;Martin 2007;Zhao et al 2007;Janarthanam & Seshadri 2008;Medina et al 2009;Rangsayatorn 2009;Hong et al 2010;Kaur & Bhutani 2010).Till date, in vitro propagation of D. chrysotoxum was reported only through seeds (immature/mature), shoot-tips and protocorm segments culture (Xu et al 2001;Roy et al 2007;Kaur & Bhutani 2011).The objective of this study was to access the regeneration competence of stemnodes to multiply the D. chrysotoxum by subjecting them to varying physical and chemical stimulus in the medium.…”

Section: Introductionmentioning

confidence: 99%

“…Apart from its high floricultural value, the species is widely used for medicinal purposes. It is rich in biologically active chemical compounds showing potent antioxidant, immune stimulating, anti-hyperglycemic (Zhao et al 2007), antiangiogenic and anti-tumoral activity (Gong et al 2004). Whole plants are used to extract cytotoxic compound, i.e.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

Kaur¹

2017

Journal of Horticultural Research

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Transverse sections (2 mm thickness) of stem-nodes from in vitro raised seedlings had morphogenic potential on semisolid and liquid Murashige and Skoog medium supplemented with cytokinins N 6 -benzyladenine (BA 4.44 μM), furfurylaminopurine (KIN 4.65 μM) and auxin α-naphthalene acetic acid (NAA 5.37 μM) individually and in combinations. The regeneration response was influenced by both the type of growth regulator and physical state of the medium. The explants produced either shoot buds on cytokinincontaining media or protocorm-like bodies (PLBs) on NAA containing media both solid and liquid. More neo-formations were produced on liquid media, especially those containing only NAA. They were formed at nodal and inter-nodal regions. The secondary buds were produced on the surface of primary PLBs. The plantlets were developed on MS medium containing banana homogenate 50 g·dm -3 . The current study is the first ever report on successful regeneration of Dendrobium chrysotoxum from stem-node segments.

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“…Um de seus principais usos é em uma infusão conhecida como "Shihu" (Yang et al, 2006), muito popular em tratamentos oftalmológicos. Estudos recentes demonstraram que as plantas de Dendrobium possuem efeitos anti-oxidantes Zhang et al, 2007), imunoestimulatórios , anti-tumorais Uma et al, 2009) e anti-hiperglicêmicos (Zhao et al, 2007).…”

Section: I2 Dendrobium "Second Love"unclassified

Efeito de giberelina, óxido nítrico e etileno no estiolamento de Dendrobium "Second Love" (Orchidaceae)

Félix¹

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DedicatóriaDedico às minhas avós, que sempre tiveram muito orgulho de mim.À meus pais, irmãos, André e Clébio, pelo amor incondicional. Epígrafe"Ama e faz o que quiseres. Se calares, calarás com amor; se gritares, gritarás com amor; se corrigires, corrigirás com amor; se perdoares, perdoarás com amor. Ao Prof. Dr. Luciano Freschi, que sempre se demonstrou disponível para ajudar-me, mesmo eu não sendo seu aluno direto. Pelas ideias valiosas que me deu para incrementar e solucionar algumas dificuldades encontradas neste experimento. Por saber que sempre poderei contar com sua ajuda, agradeço.Ao Prof. Dr. Eduardo Purgatto, por fazer tudo que estava a seu alcance para permitir o desenvolvimento deste projeto e por ter tido paciência em receber-me em seu laboratório muitas vezes ao longo destes anos. À Prof. Dra. Helenice Mercier, por seu exemplo de profissionalismo, serenidade e dedicação aos alunos.À amiga, conselheira, tutora e colega de profissão Lia Chaer, por ter tido paciência em me ensinar tudo que eu precisava aprender sobre o laboratório quando cheguei. Por me auxiliar em meus experimentos e dar ótimas dicas para desenvolvê-los da melhor forma possível. Por ter sido muito mais que colega, uma amiga. Sem sua ajuda, também não teria conseguido. Conte comigo sempre.A todos os meus colegas de laboratório. Por todas as conversas, risadas, almoços, lanches, jantas, jogos e passeios, momentos que tornaram essa caminhada muito mais agradável e descontraída. Pelas críticas construtivas e por estarem sempre dispostos a ajudar no que fosse preciso.À Ana Maria, que muitas vezes interrompeu tudo que estava fazendo para me ajudar no que eu precisasse, sempre muito disposta e disponível. Agradeço pelas conversas e amizade construída.Ao meu pai, meu exemplo, meu guia, meu conselheiro profissional e pessoal. Por ser esse homem cheio de qualidades e com um coração do tamanho do mundo. Por ser um pai exemplar e batalhador. Por estar sempre disponível para me ouvir, mesmo sempre tão cheio de coisas para fazer e resolver. Por sempre me incentivar a crescer e expandir meus horizontes. Por me amar acima de tudo, agradeço.À minha mãe, a melhor mãe do mundo. Por ser essa pessoa maravilhosa, justa, verdadeira, piedosa, carinhosa e possuir um coração imenso. Por ser exemplo pessoal e profissional como educadora. Por acreditar em um futuro melhor. Por estar sempre ao meu lado. Por ser minha amiga. Por ter abdicado de momentos seus para me educar e cuidar. Por fazer de tudo para me agradar. Por me amar acima de tudo, agradeço.Ao meu irmão, meu melhor amigo. Por me ouvir e me dar conselhos valiosos. Por colocar minha cabeça no lugar quando estou perdido. Por se preocupar comigo. Estar sempre ao meu lado. Por me incentivar a seguir a carreira que escolhi. Por ser essa pessoa que sei que posso contar sempre. Obrigado.Ao André, por acreditar em mim e me mostrar o potencial que tenho, mesmo quando muitas vezes eu mesmo não consigo ver. Por me ajudar a crescer tanto pessoalmente,

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Antioxidant and Anti-hyperglycemic Activity of Polysaccharide Isolated from Dendrobium chrysotoxum Lindl

Cited by 70 publications

References 23 publications

Hepatoprotective Effects of the Polysaccharide Isolated from <i>Tarphochlamys affinis</i> (Acanthaceae) against CCl<sub>4</sub>-Induced Hepatic Injury

Hepatoprotective Effects of the Polysaccharide Isolated from <i>Tarphochlamys affinis</i> (Acanthaceae) against CCl<sub>4</sub>-Induced Hepatic Injury

In Vitro Regeneration of Shoots From Nodal Explants of Dendrobium Chrysotoxum Lindl

Efeito de giberelina, óxido nítrico e etileno no estiolamento de Dendrobium "Second Love" (Orchidaceae)

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