Antioxidant and anti-acetylcholinesterase activities of extracts from Rapistrum rugosum in Tunisia

Amel, Omri Hichri; Malek, B.; Jannet, Hichem Ben; Ali, Lamari; Aouni, Mahjoub; Selmi, Boulbaba

doi:10.1016/s2222-1808(13)60086-9

Cited by 10 publications

(10 citation statements)

References 21 publications

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“…Inhibition of AChE and BChE are the key enzymes in the breakdown of acetylcholine and butyrylcholine that may be considered as one of the treatment approaches against several neurological disorders such as Alzheimer’s disease, senile dementia, ataxia, and myasthenia gravis. Most of the Plants have traditionally been used to enhance a cognitive function and to alleviate other symptoms associated with Alzheimer’s disease nowadays [ 1 ]. Table 3 shows the AChE and BChE percent inhibitions and IC 50 values of the A. laciniata various crude extracts using Galantamine as a positive control.…”

Section: Resultsmentioning

confidence: 99%

“…Though, human body possesses many defense mechanisms through antioxidant enzymes and non-enzymatic compounds against these oxidative stresses. But when these free radicals go out of control, the organism becomes incapable to scavenge all ROS which may lead to the development of chronic diseases, such as cancer, arteriosclerosis, nephritis, diabetes mellitus, liver injury, rheumatism, ischemia, cardiovascular and neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease [ 1 ]. Chemical agents, radiation, toxins, deep fried foods and environmental factors such as pollution, radiation, cigarette smoke and herbicides can generate these reactive free radicals [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer’s and other neurological disorders

et al. 2015

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BackgroundAtriplex laciniata L. was investigated for phenolic, flavonoid contents, antioxidant, anticholinesterase activities, in an attempt to explore its effectiveness in Alzheimer’s and other neurological disorders. Plant crude methanolic extract (Al.MeF), subsequent fractions; n-hexane (Al.HxF), chloroform (Al.CfF), ethyl acetate (Al.EaF), aqueous (Al.WtF), Saponins (Al.SPF) and Flavonoids (Al.FLVF) were investigated for DPPH, ABTS and H2O2 free radical scavenging activities. Further these extracts were subjected to acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities using Ellman’s assay. Phenolic and Flavonoid contents were determined and expressed in mg Gallic acid GAE/g and Rutin RTE/g of samples respectively.ResultsIn DPPH free radicals scavenging assay, Al.FLVF, Al.SPF and Al.MeF showed highest activity causing 89.41 ± 0.55, 83.37 ± 0.34 and 83.37 ± 0.34% inhibition of free radicals respectively at 1 mg/mL concentration. IC50 for these fractions were 33, 83 and 82 μg/mL respectively. Similarly, plant extracts showed high ABTS scavenging potential, i.e. Al.FLVF (90.34 ± 0.55), Al.CfF (83.42 ± 0.57), Al.MeF (81.49 ± 0.60) with IC50 of 30, 190 and 70 μg/ml respectively. further, H2O2 percent scavenging was highly appraised in Al.FLVF (91.29 ± 0.53, IC50 75), Al.SPF (85.35 ± 0.61, IC50 70) and Al.EaF (83.48 ± 0.67, IC50 270 μg/mL). All fractions exhibited concentration dependent AChE inhibitory activity as; Al.FLVF, 88.31 ± 0.57 (IC50 70 μg/mL), Al.SPF, 84.36 ± 0.64 (IC50 90 μg/mL), Al.MeF, 78.65 ± 0.70 (IC50 280 μg/mL), Al.EaF, 77.45 ± 0.46 (IC50 270 μg/mL) and Al.WtF 72.44 ± 0.58 (IC50 263 μg/mL) at 1 mg/mL. Likewise the percent BChE inhibitory activity was most obvious in Al.FLVF 85.46 ± 0.62 (IC50 100 μg/mL), Al.CfF 83.49 ± 0.46 (IC50 160 μg/mL), Al.MeF 82.68 ± 0.60 (IC50 220 μg/mL) and Al.SPF 80.37 ± 0.54 (IC50 120 μg/mL).ConclusionsThese results stipulate that A. laciniata is enriched with phenolic and flavonoid contents that possess significant antioxidant and anticholinestrase effects. This provide pharmacological basis for the presence of compounds that may be effective in Alzheimer’s and other neurological disorders.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer’s and other neurological disorders

et al. 2015

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“…But when these free radicals go out of control, the cell fails to eliminate these ROS which may lead to the development of chronic diseases, such as cancer, arteriosclerosis, nephritis, diabetes mellitus, liver injury, rheumatism, ischemia, cardiovascular and neurodegenerative disorders such as Alzheimer's and Parkinson's disease [15]. Studies showed that certain plants contain a large variety of substances that possess antioxidant activity [16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in Vitro Anticancer and Antioxidant Activities From Leaf Extracts of Medicinal Plant Clidemia Hirta

Narasimham

Bindu

Cheriyamundath

et al. 2017

Int J Pharm Pharm Sci

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Objective: To evaluate the anticancer and antioxidant activity of medicinal plant Clidemia hirta extracted in different solvents. Methods:Crude extracts were prepared from the leaves of Clidemia hirta using ethanol, petroleum ether and chloroform solvents. Anticancer activities and antioxidant properties were assayed using standard yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay respectively. Results:We found that the ethanol extract had higher inhibition activities against Dalton's lymphoma ascites (DLA) cancer cell line, 50% DLA cell line inhibition at 68µg/ml, while 50% inhibition by petroleum ether and chloroform extracts were at 160µg/ml and 172µg/ml, respectively. The antioxidant activity requires5µg/ml of ethanol extract to trap 50% of DPPH (IC50), whereas the positive control ascorbic acid trapped 50% of DPPH (IC50) at 3.5µg/ml. Conclusion:The prepared leaf extracts with different solvents of Clidemia hirta showed the antiproliferative and antioxidant activity in dosedependent manner. Further works is required to identify the biologically active chemical constituents, responsible for cancer cell growth inhibition from this plant.

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“…The weeds Raphanus sativus (Rs), Rapistrum rugosum (Rr), Sinapis arvensis (Sar), Nicotiana longiflora (Nl) and Dipsacus fullonum (Df), very common in the central part of Argentina, were selected as possible source of biocides. They were used previously in traditional medicine as antimicrobial compounds (Hanlon and Barnes, 2011; Pérez Gutiérrez and Pérez, 2004; Singh and Singh, 2013; Amel et al , 2013; Al-Younis and Abdullah, 2009; Sezik et al , 2001; Martinez et al , 2004; Smith and Smith, 1942; Rosendal Jensen et al , 1979).…”

Section: Methodsmentioning

confidence: 99%

“…Rr possess thiofunctionalized glucosinolates, which, according to bibliographic data, have some antimicrobial activity. The bioactivity is not attributed to intact glucosinolates but, instead, to products such as isothiocyanates, organic cyanides, oxazolidinethiones and ionic thiocyanate released upon enzymatic degradation by myrosinase (thioglucoside glucohydrolase in the presence of water) (Amel et al , 2013).…”

Section: Methodsmentioning

confidence: 99%

Natural products to control biofilm on painted surfaces

Bogdan

Deyá

Micheloni

et al. 2018

PRT

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Purpose-This paper aims to study five vegetables extracts as possible additives to control bacterial growth on indoor waterborne paints. The extracts were obtained from the weeds Raphanus sativus, Rapistrum rugosum, Sinapis arvensis, Nicotiana longiflora and Dipsacus fullonum, used in traditional medicine as antimicrobial compounds. Design/methodology/approach-Weeds extracts were characterized by Fourier transform infrared spectroscopy and UV-Vis spectrophotometry. Their antibacterial activity against Escherichia coli and Staphylococcus aureus was also determined. Afterward, selected extracts were incorporated in waterborne paint formulations. The paints' antimicrobial activity was assessed against S. aureus, monitoring biofilm formation by environmental scanning electron microscopy. Findings-As a general rule, results showed that tested paints were efficient in inhibiting biofilm formation, especially that formulated with Nicotiana longiflora. Practical implications-The tested paints can be used to protect walls from microbial colonization, which shortened coatings' useful life by discoloration and/or degradation. Concomitantly, indoor microbial colonization by aerosols could be also diminished. This is especially important in places that should have high standards of environmental hygiene, as in the food industry, health-care and sanitary centers. Orig¡na Iity/va Iue-The main value of this research was to study the antimicrobial activity of weeds extracts and to incorporate them in waterborne paints to diminish bacterial biofilm formation. This biofilm discolors and degrades the paint, and causes health problems. The use of natural compounds in coatings is increasing because of the convenience of using renewable sources, such as natural antimicrobials, in paint formulations.

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Antioxidant and anti-acetylcholinesterase activities of extracts from Rapistrum rugosum in Tunisia

Cited by 10 publications

References 21 publications

Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer’s and other neurological disorders

Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer’s and other neurological disorders

Evaluation of in Vitro Anticancer and Antioxidant Activities From Leaf Extracts of Medicinal Plant Clidemia Hirta

Natural products to control biofilm on painted surfaces

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