Antioxidant, Analgesic, Anti-Inflammatory, and Hepatoprotective Effects of the Ethanol Extract of Mahonia oiwakensis Stem

Abstract

The aim of this study was to evaluate pharmacological properties of ethanol extracted from Mahonia oiwakensis Hayata stems (MOSEtOH). The pharmacological properties included antioxidant, analgesic, anti-inflammatory and hepatoprotective effects. The protoberberine alkaloid content of the MOSEtOH was analyzed by high-performance liquid chromatography (HPLC). The results revealed that three alkaloids, berberine, palmatine and jatrorrhizine, could be identified. Moreover, the MOSEtOH exhibited antioxidative activity using the DPPH assay (IC50, 0.743 mg/mL). The DPPH radical scavenging activity of MOSEtOH was five times higher that that of vitamin C. MOSEtOH was also found to inhibit pain induced by acetic acid, formalin, and carrageenan inflammation. Treatment with MOSEtOH (100 and 500 mg/kg) or silymarin (200 mg/kg) decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared with the CCl4-treated group. Histological evaluation showed that MOSEtOH reduced the degree of liver injury, including vacuolization, inflammation and necrosis of hepatocytes. The anti-inflammatory and hepatoprotective effect of MOSEtOH were found to be related to the modulation of antioxidant enzyme activity in the liver and decreases in malondialdehyde (MDA) level and nitric oxide (NO) contents. Our findings suggest that MOSEtOH has analgesic, anti-inflammatory and hepatoprotective effects. These effects support the use of MOSEtOH for relieving pain and inflammation in folk medicine.

The medicinal uses of the genus Mahonia in traditional Chinese medicine: An ethnopharmacological, phytochemical and pharmacological review

“…The anti-inflammatory and hepatoprotective effects of MOS EtOH were found to be related to the modulation of antioxidant enzyme activity in the liver and decreases in the malondialdehyde (MDA) level and nitric oxide (NO) contents. The findings suggest that MOS EtOH has hepatoprotective effects (Chao et al, 2013).…”

“…The ethanol extracted from Mahonia oiwakensis Hayata stems (MOS EtOH ) was found to inhibit carrageenan-induced edema and inflammation in mice paw tissue. The inhibition rates ranged from 45% to 55% within 2 h after treatment with MOS EtOH (Chao et al, 2013). Inflammatory cytokines play an important role in immunologically mediated tissue damage.…”

“…The ethanol extract from Mahonia oiwakensis (MOS EtOH ), which was found to contain berberine, palmatine and jatrorrhizine, inhibits pain induced by acetic acid and formalin (Chao et al, 2013) (Table 3).…”

Evaluation of Ginsenoside Rg1 as a Potential Antioxidant for Preventing or Ameliorating Progression of Atherosclerosis

“…Briefly, MDA reacts with thiobarbituric acid at an acidic high temperature and form a stable chromophoric production. The absorbance of the production was determined at 532 nm and expressed as nmol/mg protein [9]. The activities of NOS were spectrophotometrically measured with commercially-available kits based on the oxidation of oxyhaemoglobin to methaemoglobin by nitric oxide.…”

Pharmacological Benefits of Herbal Formulations in the Management of <i>Psoriasis vulgaris<i>

“…Nowadays, it is still of significant medical interest for its analgesic, anti-inflammatory, antioxidant and hepatoprotective effects; these are due principally to its rich content in alkaloids like berberine, palmatine, jatrorrhizine, berbamine and oxyacanthine (Chao et al, 2013).…”