Antioxidant activity of twenty wild Spanish Thymus mastichina L. populations and its relation with their chemical composition

Abstract

The antioxidant activity and chemical composition of essential oils and methanolic extracts of twenty Spanish Thymus mastichina L. populations were studied. Both essential oils and methanolic extracts possessed antioxidant properties. However, the total phenol contents of the methanolic extracts varied between 2.90 and 9.15 mg GAE/g extract and the EC 25 values of DPPH free radical scavenging activity between 0.90 and 3.45 mg/mL for the methanolic extracts and 78e241 mg/mL for essential oils, these showing low antioxidant potential. Actually, in essential oils the main compound determined was the 1,8-cineole (56.8e69.6%), whereas thymol, g-terpinene, terpinolene and geraniol (species with considerable DPPH scavenging activity) were observed in low amounts. Concerning methanolic extracts, rosmarinic acid was the most abundant polyphenol (1.70e9.85 mg/g), followed by methoxysalicylic acid, apigenin, kaempferol and luteolin.

Herbicidal value of essential oils from oregano-like flavour species

“…So, an anti-anxiety (Kim, Seo, Min, Park, & Seol, 2014), antimicrobial with application as natural preservative in food packaging (Taqi, Askar, Mutihac, & Stamatin, 2013), anti-inflammatory, antiviral or inhibitory of nuclear factor (NF)-kB effect (Li et al, 2016) could be expected with marjoram essence due to high 1,8-cineol (59.59%) content. Our results are in agreement with the percentages obtained from 20 samples of T. mastichina essential oils (1,8-cineol 56.80-69.60%) from Spain (Delgado et al, 2014), and according with ISO quality standards (Table 4) of T. mastichina (Mendez-Tovar, Novak, Sponza, Herrero, & Asensio-S-Manzanera, 2016). So, marjoram essence analysed here could be this last species, known as Spanish marjoram.…”

Evaluation of Thymus vulgaris and Thymbra spicata essential oils and plant extracts for chemical composition, antioxidant, and antimicrobial properties

“…Our results agree with other studies reported for members of Lamiaceae family plants (Martins et al, ; Roby et al, ). Some researchers reported lower rosmarinic acid content (Delgado et al, ; Skendi et al, ). Erturk et al () studied Ocimum bacillicum (sweet basil) and Thymbra spicata from Amasya, Turkey.…”

“…Alizadeh, Alizadeh, Amari, and Zare () reported FRAP values for Thymus daenensis of 24.23–26.45 µM quercetin equivalent/g for dry weight. In another study, the FRAP values of Thymus mastichina were studied for both essential oil and methanolic extracts, finding that due to the use of a phosphate buffer at pH 6.6, the essential oil underwent phase separation and did not produce proper results (Delgado et al, ). The literature has no FRAP values for zahter essential oil, making our results the first, to best of our knowledge.…”

Effects of Drought Stress on Biomass, Essential Oil Content, Nutritional Parameters, and Costs of Production in Six Lamiaceae Species

“…It is characterized by leaves distributed in opposite pairs and small zygomorphic and bilabiate flowers [24]. It is an endemic thyme with a high added value due to its use as spices and/or medicinal herbs, with several pharmacological properties, such as antispasmodic, antiseptic, antitussive actions [25].…”