Antioxidant Activity of the Germinated Seed of Four Varieties of Amaranthus Caudatus L. from Peru

Aguilar-Felices, Enrique Javier; Romero-Viacava, Marta; Enciso-Roca, Edwin; Herrera-Calderón, Oscar; Común-Ventura, Pablo; Yuli-Posadas, Ricardo Ángel; Chacaltana-Ramos, Luz Josefina

doi:10.5530/pj.2019.11.93

Cited by 5 publications

(5 citation statements)

References 16 publications

(17 reference statements)

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“…The germination process of quinoa seeds is an effective technique to enhance the content of total phenols and total flavonoids and to improve the antioxidant capacity, as was demonstrated in quinoa (C. quinoa) and kiwicha (Amaranthus caudatus) [24], where the sprouts had enhanced content of coumaric acid and kaempferol tri-glycoside in quinoa and caffeoylquinic acid in kiwicha. Additionally, a significant increase was observed in the phenolic content and the antioxidant capacity through malting quinoa sprouts [25] and Amaranthus caudatus sprouts [26].…”

Section: Discussionmentioning

confidence: 97%

Biomolecules with Antioxidant Capacity from the Seeds and Sprouts of 20 Varieties of Chenopodium quinoa Willd. (Quinoa)

Enciso-Roca

Aguilar-Felices

Tinco-Jayo

et al. 2021

Plants

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Quinoa has acquired a great interest due to its high content of nutrients and biomolecules that have nutritional and medicinal properties. The aim of this study was to compare the total phenolic content (TPC), total flavonoids (TF), and the antioxidant capacity of 20 varieties of seeds and sprouts of quinoa extract. Quinoa seeds were germinated for 72 h and dried in an oven at 45 °C. The extracts were obtained by dynamic extraction using methanol. Phytochemical analysis with liquid chromatography coupled with mass spectrometry (LC-ESI-MS/MS), TPC, TF, and the antioxidant capacity was carried out and compared between both extracts. The TPC was determined with Folin-Ciocalteu reagent, TF with AlCl3, and the antioxidant capacity was determined according to the DPPH and ABTS assays. Sprout extracts showed high values of TPC (31.28 ± 0.42 mg GAE/g; Pasankalla variety), TF (14.31 ± 0.50 mg EQ/g; black Coito variety), and antioxidant capacity (IC50 (DPPH): 12.69 ± 0.29 µg/mL and IC50 (ABTS): 3.51 ± 0.04 µg/mL; Pasankalla). The extracts of the Pasankalla variety revealed 93 and 90 phytochemical constituents in the seeds and sprouts, respectively, such as amino acids, phenolic acids, flavonoids, fatty acids, and triterpene saponins, among others. Quinoa sprouts showed a high content of TPC and TF, and high antioxidant capacity compared with seed extracts, especially the Pasankalla variety.

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Section: Discussionmentioning

confidence: 97%

Biomolecules with Antioxidant Capacity from the Seeds and Sprouts of 20 Varieties of Chenopodium quinoa Willd. (Quinoa)

Enciso-Roca

Aguilar-Felices

Tinco-Jayo

et al. 2021

Plants

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“…Antioxidant activity in the ABTS assay for the germinated seeds was 151.8 mg TE/g sample for Cristalino and 151.3 mg TE/g sample for Taray, whereas, in the DPPH assay, those values were 178.1 and 180.2 mg TE/g sample, respectively. The FRAP values were found to be 132.8 and 136.4 mg TE/g of sample, respectively, for Cristalino and Taray [46]. The ethanolic extracts of A. viridis leaves were shown to inhibit LPO, to scavenge NO production, and to scavenge DPPH radicals.…”

Section: Chemical-based Assaysmentioning

confidence: 95%

A Review of Recent Studies on the Antioxidant Activities of a Third-Millennium Food: Amaranthus spp.

2020

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Amaranth (Amaranthus spp.) plant commonly refers to the sustainable food crop for the 21st century. The crop has witnessed significant attention in recent years due to its high nutritional value and agronomic advantages. It is a relatively well-balanced cosmopolitan food that is a protector against chronic diseases. Usually, the antioxidant activities of amaranth are held responsible for its defensive behavior. Antioxidant activity of plants, generally, is attributed to their phytochemical compounds. The current interest, however, lies in hydrolysates and bioactive peptides because of their numerous biological functions, including antioxidant effect. While the importance of bioactive peptides has been progressively recognized, an integrated review of recent studies on the antioxidant ability of amaranth species, especially their hydrolysates and peptides has not been generated. Hence, in this review, we summarize studies focused on the antioxidant capacity of amaranth renewal over the period 2015–2020. It starts with a background and overall image of the amaranth-related published reviews. The current research focusing on in vitro, in vivo, and chemical assays-based antioxidant activity of different amaranth species are addressed. Finally, the last segment includes the latest studies concerning free radical scavenging activity and metal chelation capacity of amaranth protein hydrolysates and bioactive peptides.

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“…A standard solution (ST) was prepared by mixing 10 mL of ABTS (4.06 mg/mL) with 10 mL of potassium persulfate (0.7 mg/mL) and reacted for 12 h. The working solution (ST) was prepared with 1 mL of each extract and 60 mL of methanol. The absorbance was adjusted to 0.7 ± 0.02 with methanol at a wavelength of 734 nm, then 150 μL of the ethyl acetate fraction of leaves and stems (5 mg/mL) were mixed with 2850 μL of the working solution and incubated in the dark for 7 min, followed by reading the absorbances at 734 nm using a Genesys 150 spectrophotometer (Thermo Scientific, Waltham, MA, USA) [ 33 ]. The standard curve was made with Trolox at 0–400 μmol/mL.…”

Section: Methodsmentioning

confidence: 99%

“…The standard curve was made with Trolox from 50 to 800 μmol/mL. The results were expressed as μmol Trolox equivalent per gram of extract (μmol TE/g of extract) [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of Jatropha macrantha Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats

et al. 2021

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Jatropha macrantha Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditional medicine as an aphrodisiac and erectile dysfunction (ED). The aim of this study was to determine the phytochemical constituents in leaves and stems ethyl acetate fraction (LEAF and SEAF) of J. macrantha and to compare the antioxidant activity and the ameliorative effect on ketamine-induced erectile dysfunction in rats. The phytochemical constituents were determined by LC-ESI-MS/MS, the total phenolic compounds and total flavonoids (TPC and TF) by Folin-Ciocalteu and aluminum chloride, respectively. The antioxidant activity was determined by DPPH, ABTS, and FRAP assays. Experimental groups were divided as follows: I: negative control; II: positive control (ketamine at 50 mg/ kg/d); III: sildenafil 5 mg/kg; IV, V, VI: LEAF at 25, 50 and 100 mg/kg, respectively, and VII, VIII, IX: SEAF at 25, 50, and 100 mg/kg, respectively. The phytochemical analysis revealed the presence mainly of coumarins, flavonoids, phenolic acids, and terpenes. TPC of LEAF and SEAF were 359 ± 5.21 mg GAE/g and 306 ± 1.93 mg GAE/g, respectively; TF in LEAF and SEAF were 23.7 ± 0.80 mg EQ/g, and 101 ± 1.42 mg EQ/g, respectively. The DPPH, ABTS, FRAP in SEAF were 647 ± 3.27; 668 ± 2.30; and 575 ± 2.86 μmol TE/g, respectively, whilst LEAF showed 796 ± 3.15; 679 ± 0.85; and 806 ± 3.42 μmol TE/g, respectively. Regarding sexual behavior, LEAF showed a better effect in mount frequency, intromission frequency, ejaculation frequency, mount latency, intromission latency, ejaculatory latency, and post ejaculatory latency than SEAF. As conclusion, LEAF of J. macrantha at 50 mg/kg showed a better effect on sexual behavior in male rats with erectile dysfunction than SEAF but not higher than sildenafil.

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Antioxidant Activity of the Germinated Seed of Four Varieties of Amaranthus Caudatus L. from Peru

Cited by 5 publications

References 16 publications

Biomolecules with Antioxidant Capacity from the Seeds and Sprouts of 20 Varieties of Chenopodium quinoa Willd. (Quinoa)

Biomolecules with Antioxidant Capacity from the Seeds and Sprouts of 20 Varieties of Chenopodium quinoa Willd. (Quinoa)

A Review of Recent Studies on the Antioxidant Activities of a Third-Millennium Food: Amaranthus spp.

Phytochemical Screening by LC-ESI-MS/MS and Effect of the Ethyl Acetate Fraction from Leaves and Stems of Jatropha macrantha Müll Arg. on Ketamine-Induced Erectile Dysfunction in Rats

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