Antioxidant activity of sulfated Porphyra yezoensis polysaccharides and their regulating effect on calcium oxalate crystal growth

Chen, Xuewu; Huang, Wei-Bo; Sun, Xin-Yuan; Xiong, Peng; Ouyang, Jing

doi:10.1016/j.msec.2021.112338

Cited by 22 publications

(18 citation statements)

References 74 publications

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“…This bioactivity was not expected on sulfated polysaccharides such as porphyran, justifiable most probably by the presence of phenols and other secondary metabolites capable of undergoing single-electron transfer reactions [55]. Porphyran extracts are reported to have low antioxidant activity in one-electron donation assays such as DPPH, with less than a 2% inhibition rate at similar concentrations [64] and in ABTS an inhibition rate in the range of 1.5-2.8% [65]. Only in aqueous extracts with a high content of phenols was this activity found to be higher [66].…”

Section: Comparative Analysis Of the Different Assaysmentioning

confidence: 99%

Development and Characterization of Films for Food Application Incorporating Porphyran Extracted from Porphyra dioica

et al. 2022

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Non-biodegradable plastic is one of the biggest environmental problems of our lifetime and, considering the present societal needs, it will get worse. Consequently, there is an urgent need to develop sustainable and renewable alternatives to plastic, such as plastic-like materials obtained from biodegradable polymers, namely sulfated polysaccharides, considered one of the most viable alternatives. There is also a need to obtain these materials in an environmentally and economically sustainable way. The hereby developed process of obtaining film-forming solutions from semi-refined porphyran (PorphSR) uses a green solvent (hot water) with a high extraction yield of semi-refined porphyran (26.66 ± 0.27%) in a reproducible way and with low levels of contaminants. The obtained semi-refined porphyran showed good antioxidant potential in all tests performed: HPSA (Δ0.066 ± 0.002), DPPH (2.23 ± 0.78%), FRAP (0.420 ± 0.014 eq. ascorbic acid µg mg−1 of extract) and ABTS (20.46 ± 0.90%). After being cast into films, the most notable antioxidant properties were those of the semi-refined porphyran in the DPPH, FRAP and ABTS assays and of the pectin, (PorphSR_PcT and PorphSR_PcT_Gly) in the HPSA assay. Morphologically, the films showed relatively homogeneous and low roughness surfaces. It is concluded that the described method to obtain semi-refined porphyran is feasible and reproducible, and that the developed films, mainly PorfP2_PcT_Gly, proved to be a potential candidate for non-biodegradable plastic substitutes.

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Section: Comparative Analysis Of the Different Assaysmentioning

confidence: 99%

Development and Characterization of Films for Food Application Incorporating Porphyran Extracted from Porphyra dioica

et al. 2022

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“… 30 In fact, some of the most effective inhibitors for calcium crystal had carboxylate (–COOH), hydroxyl (–OH) and/or sulfate (–OSO 3 –) functional groups which can interact with crystal surface in urine. 31 , 32 We therefore selected five molecules with functional groups based on the heatmaps analysis, which included Taurine (–OH), Suberic acid (–COOH), Succinate (–COOH), Salicyluric (–COOH, –OH) and Gentisic acid (–COOH, –OH) from the differentially expressed metabolites for further analysis (Fig. 2e ).…”

Section: Resultsmentioning

confidence: 99%

Application of metabolomics in urolithiasis: the discovery and usage of succinate

Zhang

Lei

Jiang

et al. 2023

Sig Transduct Target Ther

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Urinary stone is conceptualized as a chronic metabolic disorder punctuated by symptomatic stone events. It has been shown that the occurrence of calcium oxalate monohydrate (COM) during stone formation is regulated by crystal growth modifiers. Although crystallization inhibitors have been recognized as a therapeutic modality for decades, limited progress has been made in the discovery of effective modifiers to intervene with stone disease. In this study, we have used metabolomics technologies, a powerful approach to identify biomarkers by screening the urine components of the dynamic progression in a bladder stone model. By in-depth mining and analysis of metabolomics data, we have screened five differential metabolites. Through density functional theory studies and bulk crystallization, we found that three of them (salicyluric, gentisic acid and succinate) could effectively inhibit nucleation in vitro. We thereby assessed the impact of the inhibitors with an EG-induced rat model for kidney stones. Notably, succinate, a key player in the tricarboxylic acid cycle, could decrease kidney calcium deposition and injury in the model. Transcriptomic analysis further showed that the protective effect of succinate was mainly through anti-inflammation, inhibition of cell adhesion and osteogenic differentiation. These findings indicated that succinate may provide a new therapeutic option for urinary stones.

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“…PYP was obtained from algal powder of P. yezoensis by a hot water-extracted method according to our previous study. 8 , 13 , 20 …”

Section: Methodsmentioning

confidence: 99%

Regulatory Effects of Damaged Renal Epithelial Cells After Repair by Porphyra yezoensis Polysaccharides with Different Sulfation Degree on the Calcium Oxalate Crystal–Cell Interaction

Sun¹,

Zhang²,

Deng³

et al. 2021

IJN

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Background The interaction between urinary microcrystals and renal epithelial cells is closely related to kidney stone formation. However, the mechanism of cell state changes that affect crystal–cell interaction remains unclear. Methods This study investigated the relationship between the sulfate group (–OSO 3 − ) content in Porphyra yezoensis polysaccharide (PYP) and the ability to repair damaged cells, as well as the changes in cell adhesion and endocytosis of nano-calcium oxalate monohydrate (COM) crystals before and after PYP repair of damaged renal tubular epithelial cells. The sulfur trioxide–pyridine method was used to sulfate PYP (–OSO 3 − content of 14.14%), and two kinds of sulfated PYPs with –OSO 3 − content of 20.28% (SPYP1) and 27.14% (SPYP2) were obtained. The above three PYPs were used to repair oxalate-damaged human proximal tubular epithelial cells (HK-2), and the changes in the biochemical indicators of the cells before and after the repair and the changes in cell adhesion and endocytosis of nano-COM crystals were detected. Results After repair by PYPs, the cell viability increased, the number of reactive oxygen species decreased, and the reduction of mitochondrial membrane potential and the release of intracellular Ca 2+ were suppressed. The cells repaired by PYPs inhibited the adhesion of nano-COM crystals while promoting the endocytosis of the adhered crystals. The endocytosed crystals mainly accumulated in the lysosome. The ability of PYPs to repair cell damage, inhibit crystal adhesion, and promote crystal endocytosis was enhanced when the –OSO 3 − content increased. Among them, SPYP2 with the highest –OSO 3 − content showed the best biological activity. Conclusion SPYP2 showed the best ability to repair damaged cells, followed by SPYP1 and PYP. SPYP2 may become a potential green drug that inhibits the formation and recurrence of calcium oxalate stones.

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Antioxidant activity of sulfated Porphyra yezoensis polysaccharides and their regulating effect on calcium oxalate crystal growth

Cited by 22 publications

References 74 publications

Development and Characterization of Films for Food Application Incorporating Porphyran Extracted from Porphyra dioica

Development and Characterization of Films for Food Application Incorporating Porphyran Extracted from Porphyra dioica

Application of metabolomics in urolithiasis: the discovery and usage of succinate

Regulatory Effects of Damaged Renal Epithelial Cells After Repair by Porphyra yezoensis Polysaccharides with Different Sulfation Degree on the Calcium Oxalate Crystal–Cell Interaction

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