Antioxidant activity of selenium-enriched Chrysomyia megacephala (Fabricius) larvae powder and its impact on intestinal microflora in D-galactose induced aging mice

Abstract

Background: The purpose of this study was to assess the antioxidative activity of selenium-enriched Chrysomyia Megacephala (Fabricius) (C. megacephala) larvae powder (SCML) and its impact on the diversity and structure of intestinal microflora in a mouse model of D-galactose (D-gal)-induced oxidative damage. Methods: Sixty male ICR mice were equally randomized to a normal control (NC) group, a model group, a positive group, a low-dose SCML (L-SCML) group, a mid-dose SCML (M-SCML) group, and a high-dose SCML (H-SCML) group. Animals in NC and model groups received water, animals in the positive group received 40 mg/Kg vitamin E (VE), and those in the three SCML groups received SCML which include 300, 1000 and 3000 μg/Kg selenium (Se) respectively. An oxidative damage model induced by subcutaneous injection of D-gal for 6 weeks via the neck was established. Serum oxidative stress levels and tissue appearance were evaluated. Tissues oxidative stress levels were detected by commercially available kit. Nuclear erythroid 2-related factor (Nrf2) and gut microbiota were determined by western blot and high throughput sequencing 16S rRNA gene respectively. Results: An oxidative damage model was established successfully as represented by a significant elevation of malondialdehyde (MDA) and protein carbonylation, and inhibition of the antioxidants including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and glutathione (GSH). It was found that oxidative damage and histological alterations were attenuated, the expression of Kelch-like ECH-associated protein (Keap1) was decreased, and the expression of Nrf2 and hemeoxygenase-1 (HO-1) was increased after SCML treatment. In addition, significant changes were observed in the gut microbiota, including Proteobacteria and the ratio of Bacteroidetes to Firmicutes at the phylum level, as well as Helicobacter, Clostridium and Lactobacillus at the genus level.

Impact of Antibiotic-Induced Depletion of Gut Microbiota and Selenium Supplementation on Plasma Selenoproteome and Metal Homeostasis in a Mice Model

“…Proteobacteria were higher in the C–Se group than in the other groups, showing an increase in this pro-inflammatory phylum in agreement with other studies that reported a significant increase of its abundance in mice fed Se-enriched C. megacephala larvae . We also observed an increased abundance of Tenericutes phylum in Se-supplemented groups, both C–Se and Abx-Se, compared to the non-supplemented groups.…”

“…The Lactobacillus group has been observed in higher abundance in mice groups supplemented with Se, even in the antibiotic microbiota-depleted group. In this regard, it has been shown that the Lactobacillus group was increased in diets with median and high Se doses . In addition, the increase of the Lactobacillus genus has also been reported in mice fed a high-fat diet supplemented with Se compared with the un-supplemented group .…”

“…In this regard, it has been shown that the Lactobacillus group was increased in diets with median and high Se doses. 42 In addition, the increase of the Lactobacillus genus has also been reported in mice fed a high-fat diet supplemented with Se compared with the unsupplemented group. 40 Thus, while Lactobacillus is significantly reduced in antibiotic-treated mice, the Se-supplementation modulated the impact on the Lactobacillus levels in similar levels to control groups (groups Abx-Se and C).…”

Flavonoid-Rich Extract of Paeonia lactiflora Petals Alleviate d-Galactose-Induced Oxidative Stress and Restore Gut Microbiota in ICR Mice

“…On the other hand, the structure and function of the gut microbiota are critically affected by diet [ 6 ]. Increasing evidence suggested that these changes in the level of oxidative stress affected the microbial environment of the intestine and microbiota structure, which eventually caused inflammation and metabolic syndrome in living organisms [ 7 ].…”

The Hematopoietic Function of Medicinal Wine Maoji Jiu Revealed in Blood Deficiency Model Rats

“…Blood deficiency syndrome may be connected with the weak antioxidant capacity of the body [ 17 – 19 ]. The activities of T-AOC and the level of MDA in the liver are commonly detected to assess the antioxidative property in previous studies [ 13 , 20 , 21 ]. In our study, the decreased activity of T-AOC and the increased level of MDA in liver tissues in the model group were observed ( Table 3 ), which was consistent with the blood deficiency model mice in previous studies [ 10 , 21 , 22 ].…”