Antioxidant activity of purslane (portulaca oleracea l.) Leaf extract on ovarian oxidative stress levels and reproductive hormone levels of female white rats (rattus norvegicus) exposed to cigarette smoke

Abstract

Purslane (Portulaca oleracea L.) is a wild plant that grows in Indonesia and is considered a weed, but the highest antioxidant activity was found in the leaves and stems. Cigarette smoke is one of the exogenous free radicals that can enter the blood circulation, so that it can interfere with all cells and tissues in the body, including the reproductive organs. The research that will be conducted is about the antioxidant effect of purslane leaf extract on oxidative stress levels, namely ovarian malondialdehyde levels and reproductive hormone levels, namely Follicle Stimulating Hormone (FSH) and 17ß-estradiol levels in female white rats exposed to cigarette smoke. This study is an experimental study with Randomized Post Test Only Control Group Design, using 24 female white rats aged 3 months weighing 200-250 g divided into 4 groups, namely the control group (K), the cigarette smoke group (P1), the purslane leaf extract group. (P2) and the group of cigarette smoke accompanied by purslane leaf extract (P3). Ovarian MDA measurement is a measurement of MDA levels as an indicator of oxidative stress in the ovaries determined by the spectrophotometry method. Measurement of FSH and 17ß-estradiol levels in blood taken intracardially through the right ventricle of the heart as measured by the ELISA method. The results showed that purslane leaf extract at a dose of 200mg/KgBW reduced ovarian MDA levels of female white rats exposed to cigarette smoke significantly with p-value <0.05. However, there has been no significant change in FSH and 17ß-estradiol. Keywords: purslane leaf extract, cigarette smoke, Follicle Stimulating Hormone, 17ß-estradiol, malondialdehyde, health risk