Antioxidant activity of prebiotic ginseng polysaccharides combined with potential probiotic Lactobacillus plantarum C88

Abstract

The potential prebiotic properties of Panax ginseng polysaccharides were studied using ten strains of Lactobacillus plantarum isolated from traditional Chinese fermented foods. These probiotics showed different growth characteristics depending on the extract and strain specificity. L. plantarum C88 showed higher cell densities and growth rate when cultured on P. ginseng polysaccharides. In the in vitro antioxidant assay, P. ginseng polysaccharides combined with L. plantarum C88 were found to possess significant DPPH, ABTS and superoxide anion radicals scavenging activities, and acidic polysaccharides showed better antioxidant activity than neutral polysaccharides. Furthermore, we evaluated the antioxidant effect of acidic P. ginseng polysaccharide combined with L. plantarum C88 strain in natural ageing mice in vivo. Acidic P. ginseng polysaccharide and L. plantarum C88 together inhibited the formation of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and total antioxidant capacities (T-AOC) in a dose-dependent manner.

Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice

“…Mycelium polysaccharides have shown strong antioxidant as fruiting body in vitro and vivo, furthermore, researches have reported the potential use of exopolysaccharide as a novel source of prebiotics which have protective effects on the intestinal barrier function [17,18]. Accordingly, polysaccharides from cultured mycelium may be feasible for antioxidant treatment and gut microbiota regulation.…”

Preparation, characterisation and antioxidant activities of litchi (Litchi chinensis Sonn.) polysaccharides extracted by ultra‐high pressure

“…The modified method of scavenging DPPH radicals was based on the method reported by He et al . () and Song et al . ().…”

“…LCP was hydrolysed by trifluoroacetic acid (TFA, 2M) into individual monosaccharide, which was further derived by trimethylsilicane for gas chromatography (GC) analysis (Bouaziz et al, 2015;He et al, 2015;Jim enez-Escrig et al, 2015). Briefly, 10 mg dry hydrolysed polysaccharide was dissolved in 1 mL pyridine.…”

“…2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity DPPH radical, which has a very stable nitrogen centre and presents deep purple colour, is a kind of hydrophobic radical. The modified method of scavenging DPPH radicals was based on the method reported by He et al (2015) and Song et al (2016). LCP with different concentration was mixed with 2.0 mL of 0.2 mM ethanol solution of DPPH.…”

Improving the quality of Sichuan pickle by adding a traditional Chinese medicinal herb Lycium barbarum in its fermentation

“…Each 300 mg of freeze-dried sample was added into 25 mL 80% ethanol solution, crushed using homogeneous machine (AD200L-P, Onnen, Shanghai) and extracted at 60°C for 2 h. After being filtered with filter paper, all ethanol extractions were placed in a 4°C freezer for further assay. The DPPH radical scavenging capacity, total flavonoid and phenolic contents were determined according to the methods described by He et al (2015), Lee et al (2012) and Zhang et al (2015), respectively.…”