Antioxidant activity of phenolics–saponins rich fraction prepared from defatted kenaf seed meal

Abstract

The current study is aimed to determine the antioxidant properties of crude ethanolic extract (CEE) of defatted kenaf seed meal (DKSM) and its derived n-butanol (BF) and aqueous (AqF) fractions. Spectrophotometric assays showed that BF contained the highest amount of phenolic compounds and saponins, followed by CEE and AqF (p < 0.05). Similarly, HPLC-DAD analysis revealed that level of all the detected predominant phenolic compounds was significantly higher in BF (p < 0.05). Through multiple antioxidant assays, BF exhibited higher antioxidant activity than CEE and AqF, except for iron chelating activity (p < 0.05). Antioxidant activity of CEE and fractions were strongly correlated to their phenolic and saponin contents. This study showed that phenolic compounds and saponins could be extracted and partially purified simultaneously from DKSM by employing a simple alcoholic extraction-fractionation procedure. High antioxidative phenolics-saponins rich fraction from DKSM is a potential active ingredient that could be applied in nutraceuticals, functional foods as well as natural food preservatives.

Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay ( Coix lachryma-jobi L . var. ma-yuen Stapf) seed meal

“…The CAE was fractionated as follows (Chan, Iqbal, Khong, Ooi, & Ismail, 2014): the freeze-dried 80% acetone extract (4 g) was added to distilled water (100 ml) and stirred for 2 h, followed by partitioning three times sequentially with 100 ml of petroleum ether to remove residual lipids from the CAE. The aqueous layer was further partitioned three times sequentially with n-butanol (150 ml, 2 h).…”

Radical scavenging activity of extruded corn gruels with addition of linden inflorescence

“…Extracts rich in phenolic compounds can be used as medicines or preventive agents protecting humans and animals from the destructive action of free radicals [11][12][13]. Reactive oxygen species (ROS) have been implicated in many pathological conditions, including rheumatoid arthritis [14], hemorrhagic shock [15], cardiomyopathy [16] and gastrointestinal ischemia [17].…”

Effect of moisture on the physical properties of three varieties of kenaf seeds

“…Kenaf plants have been widely used for the production of paper, biocomposites, fiber boards and bioplastics and in the textile industry. Depending on the variety and agroclimatic conditions, kenaf seed is an agricultural by-product of kenaf plantation, which yields up to 1,000 kg/ha (Chan et al 2014). Kenaf seeds have potential uses in soap manufacturing and in linoleum, paints, varnishes, bio-diesel and industrial lubricants (Agbaje 2010).…”