Antioxidant activity of Nigella sativa essential oil

Burits, M.; Bučar, Franz

doi:10.1002/1099-1573(200008)14:5<323::aid-ptr621>3.0.co;2-q

Cited by 1,496 publications

(952 citation statements)

References 20 publications

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“…The 2, 2 diphenyl-1-picrylhydrazyl (DPPH) test was carried out as described by Burits and Bucar [20] . One mL of algal extract (100 毺g/mL) was mixed with 1ml DPPH reagent (0.002% (w/v) /methanol solution).…”

Section: Antioxidant Activity 241 Dpph Methodsmentioning

confidence: 99%

Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

Shanab

Mostafa

Shalaby

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

162

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Section: Antioxidant Activity 241 Dpph Methodsmentioning

confidence: 99%

Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

Shanab

Mostafa

Shalaby

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

162

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“…DPPH spectrophotometric assay DPPH scavenging activity in the extracts was assessed as described by Burits and Bucar (2000). Each extract stock solution (1.0 mg/ml) was diluted to final concentrations ranging from (10-50 μg/ml) in EtOH.…”

Section: Methodsmentioning

confidence: 99%

“…Reactive oxygen species (ROS), causing damage to DNA, proteins and lipids, have been associated with carcinogenesis, coronary heart disease and many other health problems (Black et al 1995;Cadenas and Davies 2000). Minimizing oxidative damage may well be one of the most important approaches to prevention of these oxidative stress-related diseases and health problems, since antioxidants terminate direct ROS attacks and radicalmediated oxidative reactions (Burits and Bucar 2000;Yu et al 2002). The auto oxidation of fats is a major problem due to deterioration in the quality of foods mediated oxidative reactions.…”

Section: Introductionmentioning

confidence: 99%

Effects of temperature and solvent on antioxidant properties of curry leaf (Murraya koenigii L.)

Sasidharan

Menon

2010

J Food Sci Technol

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Total polyphenol content (TPC) and antioxidant activities of curry leaf extracts (hexane, chloroform, ethanol, ethanol-water (1:1) and water at ambient (AT, 25°C) and boiling temperature (BT) (Soxhlet extraction), were determined by DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt), and total reductive potential assays. TPC was in the order ethanol-water (1:1) (AT) > water (AT) > chloroform (AT) > ethanol-water (1:1) (BT) > hexane (AT) > ethanol (BT) > water (BT) > hexane (BT). Ethanol-water (AT) had the maximum TPC of 501± 4.6 mg/g GAE and 82% radical scavenging activity (RSA) at 10 μg/ml level (DPPH) and 100% RSA (ABTS) at 10 μg/ml and at hot conditions (ethanol-water (BT)) had less TPC (28.7±0.9%), and 43% RSA by DPPH and 53.6% by ABTS assays. Hot extracts had lesser antioxidant activities than ambient extracts. The best solvent system for getting maximum antioxidant activity from curry leaves was ethanol-water (1:1)-(AT).

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“…This spectrophotometric assay uses the stable radical, 1,1-diphenyl-2-picrylhydrazyl (DPPHz), as a reagent [36]. The method of Blois [37] previously described by Gü lçin [3] was used with slight modifications in order to assess the DPPH· free radical scavenging capacity of silymarin.…”

Section: Dpphz Free Radical Scavenging Activitymentioning

confidence: 96%

In vitroantioxidant activity of silymarin

Köksal

Gülçın

Beyza

et al. 2009

Journal of Enzyme Inhibition and Medicinal Chemistry

197

100

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Silymarin, a known standardized extract obtained from seeds of Silybum marianum is widely used in treatment of several diseases of varying origin. In the present paper, we clarified the antioxidant activity of silymarin by employing various in vitro antioxidant assay such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH·) scavenging, 2,2 0 -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination by Fe 3þ 2 Fe 2þ transformation method and Cuprac assay, superoxide anion radical scavenging by riboflavin/methionine/illuminate system, hydrogen peroxide scavenging and ferrous ions (Fe 2þ ) chelating activities. Silymarin inhibited 82.7% lipid peroxidation of linoleic acid emulsion at 30 mg/mL concentration; butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), a-tocopherol and trolox indicated inhibition of 83.3, 82.1, 68.1 and 81.3% on peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, silymarin had an effective DPPH· scavenging, ABTS zþ scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe 3þ ) reducing power by Fe 3þ 2 Fe 2þ transformation, cupric ions (Cu 2þ ) reducing ability by Cuprac method, and ferrous ions (Fe 2þ ) chelating activities. Also, BHA, BHT, a-tocopherol and trolox, were used as the reference antioxidant and radical scavenger compounds. Moreover, this study, which clarifies antioxidant mechanism of silymarin, brings new information on the antioxidant properties of silymarin. According to the present study, silymarin had effective in vitro antioxidant and radical scavenging activity. It could be used in the pharmacological and food industry because of its antioxidant properties.

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Antioxidant activity of Nigella sativa essential oil

Cited by 1,496 publications

References 20 publications

Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

Effects of temperature and solvent on antioxidant properties of curry leaf (Murraya koenigii L.)

In vitroantioxidant activity of silymarin

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