Antioxidant Activity of nHexane Extract of Caryota no Seed Using Drosophila melanogaster Model

Abstract

<jats:p>Aims: To investigate the anti-oxidant activity of the nhexane extracts of Caryota no seeds in D. melanogaster.&#x0D; Study Design: Experimental design.&#x0D; Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019.&#x0D; Methodology: Total protein assays were carried out by exposing 50 flies in each vial to the following concentrations: 300 mg, 350 mg, 400 mg, 500 mg and 600 mg of nhexane extracts in 5 replicates for 7 days with daily recording of mortality. In vivo antioxidant activity study was conducted by measuring levels of Glutathione-S-Transferase (GST), catalase (CAT) and total thiol (TT) from supernatants of whole fly homogenates using a spectrophotometer at specific wavelenghts. The values were derived as part of the total protein value. The statistical difference among test groups was presumed at P &lt;0.05. &#x0D; Results: The nhexane extract of CN caused nonsignificant (P = .52) changes in total protein levels compared to the control. The antioxidant activity showed nonsignificant (P = .64) higher total thiol contents and GST (P = .76) activity in nhexane extract-treated flies and lower catalase (P = .30) activity was recorded in the nhexane extract-treated flies compared to the controls.&#x0D; Conclusion: It can therefore be concluded that the nhexane extract of Caryota no possess appreciable concentrations of different types of antioxidants.</jats:p>

Effect of Methanolic Extract of Caryota no on Antioxidant Levels in Drosophila melanogaster

“…2) as the extract concentration rises, the levels sharply rose with the highest extract concentration. Contrary to observations with the nhexane extract of CN [20], there was a nonsignificant (P = .28) lowering of the GST levels in DM below basal or control levels and also in an inverse dose-dependent manner. The higher the methanolic extract dose, the smaller the GST concentration and vice versa.…”

“…There was an obvious inverse relationship between protein concentration in the fly and the dose of the administered extract with least extract dose having the highest protein level while the highest extract dose presents the least protein concentration. This pattern was also seen with the nhexane extract of CN [20].…”

“…3). The pattern of effect of the methanolic extract of CN on thiol is exactly as observed with the nhexane extract of CN [20]. It can be inferred that the methanolic extract of CN at high dose is a veritable source of exogenous thiol antioxidant.…”

“…The one-way ANOVA summary revealed P = .36. The methanolic extract of CN produced an opposite constellation of effects in catalase levels from the observations with the nhexane extract of CN [20]; causing slight increases above basal levels in a direct dosedependent pattern. It can be inferred that the methanolic extract of CN at high dose is a veritable source of exogenous catalase antioxidant.…”

“…Indeed, cellular macromolecules are protected primarily from the insult of free radical species by endogenous antioxidant molecules including catalase, glutathione peroxidase, glutathione-Stransferase, and superoxide dismutase [24]. There was sharp elevation in the GST activity of C. no nhexane extract-treated flies [20].…”