Evaluation of the Pumpkin Seed Membrane as a Nutrient in the Fruit Fly Model, Fed With High Fat Diet

Abstract

The pumpkin seed membrane (PSM), which is high in water retention in the study, was recycled as waste, intended to be used in terms of target-non-target creatures and to determine its effect on resistance formation. Nowadays, when fat intake is steadily increasing through nutrition, increased use of fat makes an individual vulnerable to stress. The study determined the effect of additional taken waste/nutrients on oxidative stress in obese individuals. The fatty diet and pumpkin seed effect were trialed in the model organism in the study. The amount of Malondialdehyde (MDA) and total oxidation (TOS) and total antioxidant effect (TAS) and glutathione-S-transferase (GST) activity in larvae, pupae, and adult of Drosophila melanogaster were calculated by adding different proportions of PSM (0.05-2 g) and fat (20%) to the diet. According to the data obtained: It was determined that the period with the highest oxidative stress index (OSI) is the pupae (pupae> larvae> female> male), the amount of lipid peroxidation (MDA) is higher in females, moreover, the third larvae is the most resistant stage. The results have shown that waste of seed mambrane can't be useful in vivo as a nutrient due to usage limitation.