Antioxidant activity of N-acetylcysteine, flavonoids and α-tocopherol on endometrial cells in culture

Estany, Susanna; Palacio, J.; Barnadas, Ramon; Sabés, M.; Iborra, A.; Martı́nez, Paz

doi:10.1016/j.jri.2007.01.007

Cited by 36 publications

(31 citation statements)

References 41 publications

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“…In the present study, exposure of H 2 O 2 to EPCs had induced oxidative damage and loss of cell viability. This fi nding is in accordance with previous results that reported the oxidant-mediated cellular injury by H 2 O 2 on human umbilical vein endothelial cells and other cell types like human fi broblast cells and endometrial cells (Spencer et al, 2001;Estany et al, 2007;Cianchetti et al, 2008). As far as the results of the current study are concerned, the harmful effect of H 2 O 2 and the protective effect of GB are mainly related to their oxidant and antioxidant activity.…”

Section: Y Tang Et Al Ginkgolide and Bone Marrow-derived Endothelialsupporting

confidence: 93%

Ginkgolide B promotes proliferation and functional activities of bone marrow-derived endothelial progenitor cells: involvement of Akt/eNOS and MAPK/p38 signaling pathways

Tang

Huang²,

Sun³

et al. 2011

eCM

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Bone marrow-derived, circulating endothelial progenitor cells (EPCs) contribute to neovascularization in various diseases, and represent a very interesting alternative cell source for enhancing vasculogenesis in regenerative medicine. In this study, we investigated the effects of Ginkgolide B (GB) on proliferation and differentiation of EPCs, and the involved signaling pathway in vitro. EPC proliferation, migration, adhesion and angiogenesis activities were assessed with the WST-8 assay, Transwell chamber assay, cell counting and angiogenesis kit, respectively. Apoptosis was detected with annexin V and propidium iodide staining. The protein expression of angiogenesis-related makers was detected by Western blot, and related gene expression was determined by real-time polymerase chain reaction (RT-PCR). The results showed that GB promoted the proliferation and endothelial gene expression, and markedly enhanced vascular endothelial growth factor-induced migration response and the capability to incorporate into the vascular networks in EPCs. GB protected EPCs from H 2 O 2 -induced cell death. GB induced the phosphorylation of eNOS, Akt and p38, which in turn promoted cell proliferation and function. In conclusion, the present study demonstrates that GB, at a near medical applied dose, increases the number and functional activities of EPCs with involvement of Akt/endothelial nitric oxide synthase and mitogen-activated protein kinase (MAPK)/ p38 signal pathways. These findings raise the intriguing possibility that GB may play an important role in the protection and revascularization of blood vessels.

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Section: Y Tang Et Al Ginkgolide and Bone Marrow-derived Endothelialsupporting

confidence: 93%

Ginkgolide B promotes proliferation and functional activities of bone marrow-derived endothelial progenitor cells: involvement of Akt/eNOS and MAPK/p38 signaling pathways

Tang

Huang²,

Sun³

et al. 2011

eCM

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“…At moderate doses, recovery of normospermic conditions may occur within 1-3 years; however, increased exposure can result in extended or permanent azoospermia (Claeson et al 2000;Cheng et al 2002;Doganay et al 2006;Ciftci et al 2013;Delbes et al 2010). Premature menopause and infertility may result from acute exposure to chemotherapy regimens including anthracycline and CYC (Du et al 2010a;Estany et al 2007). DOX exposure is known to increase oocyte apoptosis in cell culture based on experiments (Estany et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Premature menopause and infertility may result from acute exposure to chemotherapy regimens including anthracycline and CYC (Du et al 2010a;Estany et al 2007). DOX exposure is known to increase oocyte apoptosis in cell culture based on experiments (Estany et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of chemotherapy exposure prior to pregnancy on fetal brain tissue and the potential protective role of quercetin

et al. 2014

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Cyclophosphamide (CYC) and doxorubicin (DOX) are among the most effective and widely used anticancer chemotherapeutic drugs. Potential chemopreventive and chemotherapeutic functions have recently been attributed to flavonoids. We hypothesized that Quercetin (QR) would protect against the toxic effects of chemotherapeutic agents applied prior to pregnancy. Rats were treated with the chemotherapeutic drugs CYC (27 mg/kg) and DOX (1.8 mg/kg) applied in a single intraperitoneal dose once every 3 weeks for 10 weeks. QR was administered at a dose of 10 mg/kg/day by oral gavage. 48 h following the experimental chemotherapy exposure, female rats were transferred to cages containing male rat for mating. Fetal brain tissues were removed from fetuses extracted by cesarean section on the 20th day of gestation for evaluation of antioxidant parameters. A significant increase in superoxide dismutase and malondialdehyde activity was observed in CYC and DOX treatment groups relative to the control group (p \ 0.05). Similarly, carnitine acylcarnitine translocase and Glutathione activity was significantly reduced in the CYC and DOX groups relative to the control group (p \ 0.05). Our results indicate that the use of chemotherapeutic drugs before pregnancy can result in oxidative damage to fetal brain tissue. Therefore, women who have been exposed to chemotherapy and may become pregnant should be treated with antioxidant compounds such as QR to reduce the risk of damage to fetal brain tissues.

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“…They, also, protect macrophages from oxidative stress by keeping glutathione in its reduced form (10). Flavonoids have the capacity to inhibit enzymes such as cyclooxygenases and protein kinases involved in cell proliferation and apoptosis (11). They, also, protect normal rat islets from STZ, normalize blood glucose levels and promote â-cell regeneration in islets of STZ-treated rats (12).…”

Section: Introductionmentioning

confidence: 99%

Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats

Abdelmoaty

Ibrahim

Ahmed

et al. 2010

Indian J Clin Biochem

117

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Antioxidant activity of N-acetylcysteine, flavonoids and α-tocopherol on endometrial cells in culture

Cited by 36 publications

References 41 publications

Ginkgolide B promotes proliferation and functional activities of bone marrow-derived endothelial progenitor cells: involvement of Akt/eNOS and MAPK/p38 signaling pathways

Ginkgolide B promotes proliferation and functional activities of bone marrow-derived endothelial progenitor cells: involvement of Akt/eNOS and MAPK/p38 signaling pathways

Effect of chemotherapy exposure prior to pregnancy on fetal brain tissue and the potential protective role of quercetin

Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats

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