Antioxidant activity of methanol extract of cassava leaves (Manihot esculenta Crantz) using CUPRAC method

Abstract

Background: Antioxidants are a group of organic molecules and enzymes that work synergistically to enhance cellular defenses and combat oxidative stress. Antioxidants can come from plants because they contain many groups of phytochemical compounds and vitamins. One of the plants that has compounds with antioxidant activity is cassava. Objective: To measure the antioxidant activity of methanol extract of cassava leaves using the CUPRAC method. Methods: Cassava leaves were extracted by maceration using methanol as a solvent with the addition of 5% H2SO4. Phytochemical screening of flavonoids with Mg and HCl, while phenol with the addition of FeCl3. The antioxidant activity based on the reaction of the test solution with CUPRAC reagent. Antioxidant activity can be determined by IC50 value by using x value as concentration and y value as absorbance. Results: The results of phytochemical screening showed that the methanol extract of cassava leaves contained flavonoids and phenolic groups. The antioxidant capacity of the methanol extract of cassava leaves obtained an IC50 value of 156.03 ppm, while quercetin has a very strong capacity as an antioxidant with an IC50 of 9.83 ppm. Conclusion: The methanol extract of cassava leaves has a weak antioxidant capacity in reducing metal ions. Keywords: Cassava leaves, Manihot esculenta Crantz, methanol extract, antioxidants, CUPRAC.

Comparison of maceration and infundation towards antioxidant capacity of leaves aqueous extracts of balik angin (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz)

“…This solution is then incubated for 30 minutes in a dark place. After incubation, the absorbance of the solution was measured using a UV-Vis spectrophotometer at a wavelength of 400-600 nm (Apak et al, 2014;Sayakti et al, 2022).…”

“…The mixture is incubated at room temperature and in the dark for 30 minutes. Absorbance was measured using a UV-Vis Spectrophotometer at the maximum wavelength obtained (Apak et al, 2014;Sayakti et al, 2022).…”

Pengaruh Pemberian Ekstrak Daun Singkong (Manihot Esculenta) Dosis Bertingkat Terhadap Hemoglobin Dan Hematokrit Mencit Yang Terpapar D-Allethrin

“…Dari hasil penelitian ini, skrining fitokimia tanaman singkong ditemukan beberapa senyawa aktif berupa triterpenoid dengan rf 0,35 dan 0,43, alkaloid dengan rf 0,71 dan 0,78, flavanoid dengan rf 0,54 dan 0,78, selain itu juga ada tanin, dan saponin, dimana nilai rf masing masing senyawa menunjukan nilai positif pada rentang rujukan 0,2-0,8 berdasarkan penelitian yang dilakukan oleh (Sari & Meitisa, 2017) Pelaksanaan skrining fitokimia dengan metode kromatografi kertas dua dimensi mendapatkan hasil golongan flavonol 3-O-Glikosida sekaligus dengan skrining kromatografi lapis tipis dengan hasil RF yang di dapatkan senilai (0,82).…”

Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Tigaron (Crateva Religiosa) Menggunakan Metode DPPH

“…Perhitungan nilai IC50 untuk mendapatkan persamaan regresi linier y= bx + a yang diperoleh dari nilai konsentrasi sampel dan % inhibisi. Semakin rendah nilai IC50 yang didapatkan maka semakin baik aktivitas antioksidannya (Sayakti et al, 2022). Sehingga diperoleh nilai IC50 dari ekstrak etil asetat daun Tigaron religiosa) sebesar 114,0457 ppm yang termasuk kedalam golongan sedang (<150 ppm).…”