Antioxidant activity of lignin phenolic compounds as by-product of pretreatment process of bioethanol production from empty fruits palm bunch

Abstract

Abstract.As by-product of pretreatment bioethanol production, ligno-cellulosic biomass creates an abundance of bioresidue. This work is devoted to studies the antioxidant activity of lignin that obtained from recovery process of bioethanol by-product. This by-product comes from pretreatment process of empty fruit palm bunch in acid (pH 2) and alkaline (pH 12) conditions. The samples of purified lignin were characterized by Fourier Transform Infrared (FTIR) and Particle Size Analyzer (PSA). Radical scavenging efficiency of lignin was examined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method using quercetin as a standard. The value of IC50 showed that the lignin that was purified in acid condition (pH 2) gave the activity value in antioxidant active range (IC50 sample Lignin pH 2 = 69.41), on the other hand the lignin that was purified in alkaline condition (Lignin pH 12) did not have the activity value as an antioxidant (IC50 sample Lignin pH 12 = NA).

Identifikasi Logam pada Endapan Lindi Hitam Hasil Samping Proses Bioetanol sebagai Salah Satu Konsep dalam Pemurnian Lignin

“…industri kebutuhan level kemurnian bervariasi tergantung pada fungsi lignin yang dituju, antara lain sebagai perekat, [10] sebagai bahan kosmetik. [11] Ini ada hubungannya dengan kandungan selain lignin dalam lindi hitam dapat berupa senyawaan organik (seperti: selulosa dan hemiselulosa selulosa) atau bahan anorganik (logam dan silika), namun dalam penelitian ini karakterisasi difokuskan untuk melihat kemurnian padatan lignin ditinjau dari jumlah pengotor anorganik terutama logam berbahaya.…”

“…The TPC and EDS results show that the sample prepared at the pH = 12 has 28.9% ash containing nine elements range from 1. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].0%, whereas it is at the pH = 1 has 0.9% ash containing four inorganic elements only ranges from 0.2-1.8 %. The average of particle size of sample is decreasing from 262 µm to 59 µm with the increasing of the precipitation from pH = 1 to pH = 12.…”

Innovative Sustainable Wood Preservatives From Pulp and Paper Industry Byproduct

“…Bhat et al precipitated lignin from palm oil black liquor using sulfuric acid, they observed anti-radical activities against DPPH of approximately 62 and 65% at 0.05 and 0.1 mg/ml respectively using dimethyl sulfoxide as a solvent; and antiradical activity of approximately 80 and 78% at 0.05 and 0.1 mg/ml respectively using 2methoxy ethanol as a solvent [255]. Meliana and Setiawan studied the effect of precipitation pH and treatment concentration on the anti-radical activity of palm fruit lignin from bioethanol byproduct, they found an anti-radical activity against DPPH of 39.3 and 54.9 % for lignin precipitated at pH 2, and 14.4 and 15.1 % for lignin precipitated at pH 12 using methanol as the solvent [256]. Gordobil et al reported anti-radical activities of approximately 68% and 52% for softwood (precipitated at pH 2) and hardwood kraft lignin at a concentration of 0.037 mg/ml in methanol [257].…”