Antioxidant Activity of Honey Mushrooms (Armillaria mellea)

Abstract

Mushrooms Basidiomycota have long been appreciated for their taste, flavour, desirable aroma, texture, nutraceutical and medicinal attributes. Honey mushrooms (Armillaria mellea) are edible mushroom generally used in traditional oriental medicine. The aim of this study was to examine extracts from the fruiting bodies of these mushrooms. The content of the components with antioxidant properties, such as total phenols, total flavonoids, β-carotene, lycopene and β-glucans were determined by spectrophotometric methods. The results obtained showed that the water extracts contained higher levels of total phenols and total flavonoids (367.1 and 548.5 mg.dm−3 gallic acid equivalent — GAE, respectively) in comparison with methanol extracts (108.2 and 113.4 mg.dm−3 GAE, respectively). Very low contents of β-carotene and lycopene were determined in the methanol extract (0.756 mg.g−1 dry weight and 0.05 mg.g−1 dry weight, respectively). Methanol extracts from the fruiting bodies of Honey mushrooms (Armillaria mellea) inhibited the uptake activity of 2,2-diphenylpicrylhydrazyl (DPPH) free radicals by 45 %. The IC50 (mg of compound, that inhibit 50 % of DPPH radicals) of methanol extract was below 10 mg.cm−3 (6.448 mg.cm−3), suggesting a high antioxidant potential of fruiting bodies of the Honey mushrooms Armillaria mellea.

Chemical Composition, Bioactive Compounds, and Antioxidant Activity of Two Wild Edible Mushrooms Armillaria mellea and Macrolepiota procera from Two Countries (Morocco and Portugal)

“…The amount of β-carotene and lycopene in A. mellea of PT was significantly higher than the MA one, while in M. procera , the amount of MA was higher than PT ( Table 1 ). These results are different or near to previous studies reported by other authors [ 25 , 26 , 37 , 38 , 39 , 46 ].…”

“…Total phenolic content (TPC) of methanolic extract of A. mellea from MA (20.61 mg GAE/g dme) was observed to be similar to that noted in Turkey [ 46 ] and Serbia [ 36 ], while showing a higher value in comparison with the one from PT (12.52 mg GAE/g dme). The studies from Poland [ 28 ] and Slovak Republic [ 37 ] reported a lower amount than the obtained for our studied samples. For TPC in M. procera , it was found that the sample from PT had significantly higher content (21.46 mg GAE/g dme) than the sample from MA (12.24 mg GAE/g dme).…”

“…Concerning the ascorbic acid content, the fruiting bodies provided moderated results (2.25 to 2.99 mg AAE/g dw), which were higher by about the double than the previous studies reported by the authors [ 31 , 37 , 39 ].…”

“…As presented in Table 1 , the yields of methanolic extract of the fruiting body of A. mellea were significantly similar for the samples from Northern Morocco (MA) (33.5%) and Portugal (PT) (32.78%) and near to the Slovak one (35.2%) [ 37 ]. Similarly for M. procera , there was no significant difference between the yield of MA (34.10 ± 1.7%) and PT (34.33 ± 0.96%), but they were higher than the previous studies [ 23 , 39 , 41 ].…”

“…There are some previous studies on chemical compositions, bioactive compounds, and biological compounds of A. mellea and/or M. procera from different countries such as China [ 10 , 21 ], Croatia [ 22 ], India [ 23 , 24 , 25 ], Poland [ 26 , 27 , 28 ], Portugal [ 29 , 30 , 31 , 32 , 33 ], Romania [ 34 ] Serbia [ 35 , 36 ], Slovak Republic [ 37 ], Tanzania [ 38 ], and Turkey [ 39 , 40 , 41 ].…”