Antioxidant activity of extracts with extraction methods from Phellinus linteus mycelium on Mori ramulus

Abstract

This study was conducted to examine the antioxidant activities of extracts derived with different extraction methods (HE, hot water extraction; UE, ultrasonic extraction; and PE, pressured extraction) from Phellinuslinteus mycelium in Mori ramulus. The extraction yield of PE (7.73%) was higher than that of UE (6.03%) and of HE (5.44%). The total phenolic and flavonoid contents of PE were 9.87 g/100 g and 1.90 g/100 g, respectively. The oxygen radical absorbance capacity (ORAC) of PE (769.63 μM/g FW) was higher than that of HE (622.96 μM/g FW) and of UE (249.06 μM/g FW). The DPPH and ABTS radical scavenging activities of PE at 1,000 μg/mL were 35.85% and 51.42%, respectively. The superoxide radical scavenging activity of PE, 29.16-93.89%, was higher than that of other extracts. The ferric-reducing antioxidant power and the reducing power of PE were 180.60-607.93 μM and 0.14-0.51, respectively. The tyrosinase inhibition activity of PE (9.66-24.05%) improved with an increase in the treatment concentration. The antioxidant and tyrosinase inhibition activities of PE were significantly higher than those of the other extracts. In conclusion, we provided experimental evidence that Phellinus linteus mycelium extracts from Mori ramulus have potential as functional materials. Key words：Phellinus linteus

Influence of autoclave treatment and enzymatic hydrolysis on the antioxidant activity of Opuntia ficus-indica fruit extract

“…In addition, it is assumed that the increase of soluble fiber caused by the disruption of the insoluble cell wall under high pressure enhanced the solid extraction yield in the AE. These results are in close agreement with the previous study [8], which indicated that autoclave extraction was more efficient than water extraction with increasing extraction yield from Phellinus linteus mycelium. In fact, there are some examples in which localized pressure application has proven to be sufficient to the collapse cell wall structures such as in the ultrasonic extraction method [19].…”

“…To determine the appropriate extraction method, multiple parameters such as the type of solvent, solvent-solute ratio, total extraction time, and temperature must be considered, which can affect the yield, composition, and bioactivity of the extract [7]. Among various extraction methods, autoclaving has been recently introduced and has been proven to extract bioactive substances more efficiently since it utilizes the higher pressure and temperature than other methods do, resulting in an increase in extraction yield [8]. In other words, by applying pressure at high temperature using an autoclave possibly enhances the contact between the solvent and raw material, resulting in an increase of extraction yield.…”

Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus: A review

“…Superoxide radical scavenging activity and tyrosinase inhibitory activities of pressured extracting fractions were significantly higher than hot water and ultrasonic extracting fractions of PL [159]. The ability of chelating ferrous ions, anti-acetylcholinesterase, inhibitory activities antioxidant and xanthine oxidase of fruiting body extract was better than that from mycelia and culture filtrates [161].…”

Submerged cultivation and phytochemical analysis of medicinal mushrooms (Trametes sp.)

“…It is important to note that the chemical composition of macrofungal biomass is strain-specific and strongly dependent on the environment and cultivation technique ( Bains et al., 2021 ). These days, numerous techniques can help in the extraction process, such as methanol extraction ( Sezer et al., 2017 ), enzyme assistance, microwave assistance, ultrasonic assistance, ethanol extraction ( Bae et al., 2015 ; Angelova et al., 2022 ), and hot water extraction ( Park and Hong, 2014 ). In this study, we used an 80% ethanol extraction method to extract pure phytochemicals and polysaccharides from MBE and BE.…”