Antioxidant activity of extracts from Schinus molle L. and Gleditsia triacanthos L.

Factorial design and Response Surface Methodology (RSM) were used to model the recovery of polyphenols from Schinus molle L. peel (SM) by solvent extraction. Experiments carried out according to a Central Composite Design (CCD) allowed evaluation of the effects of temperature, extraction time, solvent composition and solid to liquid ratio on the yield of polyphenol. Statistical analysis showed that temperature was the most influential factor. A reduced polynomial model was developed by the stepwise regression method. The model was checked by performing validation experiments both inside and outside the factorial region. A very good agreement was observed between experimental and calculated extraction yields, thus supporting the use of the model to quantitatively describe the recovery of polyphenols from SM. Keywords Schinus molle • Polyphenols • Factorial design and response surface method Abbreviations RSM Response surface methodology SM Schinus molle CCD Central composite design DPPH 1,1-diphenyl-2-picrylhydrazyl TPC Polyphenol compounds yield GAE Gallic acid equivalent DM Plant dry matter X1 The non-coded values for temperature X2 The non-coded values for time X3 The non-coded values for solvent extraction X4 The non-coded values for solid to liquid ratio A Absorbance IC 50 Inhibition concentration at 50% (T) Factor temperature (p) Factor solid to liquid ratio (S)

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“…The antiradical activity of essential oils and extracts was attributed to the presence of phenolic compounds. [19,24]…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Optimization of Polyphenols Recovery from Schinus molle L. Peel Using Response Surface Methodology (RSM)

et al. 2020

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“…The evaluation of antioxidant activity and the determination of IC50 values of various extracts obtained from the leaves and stem bark of X. strumarium were achieved DPPH radical scavenging assay by using the method described in the literature (Pillai & Magama, 2020;Pillai et al, 2018;Sixtus & Pillai, 2022). The preparation of stock solutions of each extract and positive control, ascorbic acid (3.0 mg of each extract or ascorbic acid in 1.0 ml of 50% methanol, v/v) and further dilutions from each of these stock solutions (3000, 2000, 1500, 1000, 800, 500, and 200 µg/ml), the preparation of negative control (50% methanol blank solution, v/v) and rest of the experimental procedure were as per the details outlined in the literature (Sixtus & Pillai, 2022).…”

Section: Dpph Radical Scavenging Activity and Determination Of Ic50 V...mentioning

confidence: 99%

Antioxidant activity of extracts from Xanthium strumarium – A medicinal plant from the Kingdom of Lesotho

Karuppiah Pillai,

Thebe,

Matamane

2023

ijpbp

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Xanthium strumarium L. finds therapeutic applications in traditional medicines. The objective of the current study was to evaluate the antioxidant activity and to determine the total phenolic contents (TPCs) and total flavonoid contents (TFCs) of hexane, chloroform, ethyl acetate, acetone, methanol, and water extracts obtained from the leaves and stem bark of X. strumarium. Maceration and hot solvent extraction techniques were used to obtain various solvent extracts. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing power assays were used to evaluate the antioxidant activity. Folin-Ciocalteu colorimetric and aluminum chloride colorimetric methods were used to determine the TPCs and TFCs, respectively. The extracts from the leaves and stem bark exhibited radical scavenging activity in the ranges of 18.06 ± 0.3-185.67 ± 11.54% and 9.13 ± 0.54-84.18 ± 0.92%, respectively at a concentration range of 200-3000 µg/ml. The positive control, ascorbic acid, exhibited radical scavenging activity in a range of 56.64 ± 1.26-88.98 ± 0.31% at a concentration range of 200-3000 µg/ml. Additionally, the IC50 values of all these extracts were determined. The hexane and chloroform extracts from both leaves and stem bark and methanol leaf extract were found to be the most potent extracts with an IC50 value of < 200 µg/ml for each extract. The IC50 value of positive control, ascorbic acid was determined to be < 200 µg/ml. Furthermore, in the ferric-reducing power assay, ethyl acetate extract from both leaves and stem bark exhibited the highest ferric-reducing power of 0.996 ± 0.101 and 0.947 ± 0.018 at a concentration of 100 µg/ml. Moreover, the methanol extract from the leaves showed the highest TPCs of 133.41 ± 3.23 mg GAE/g of DW of extract followed by the methanol extract from stem bark and the acetone extract from the leaves with TPCs of 121.21 ± 3.14 and 118.01 ± 1.85 mg GAE/g of DW of extract, respectively. Similarly, the methanol extract from the leaves also showed the highest TFCs of 20.61 ± 1.81 mg QE/g of DW of extract followed by the methanol extract from stem bark with TFCs of 14.90 ± 1.18 mg QE/g of DW of extract. From this study, we concluded that various extracts obtained from the leaves and stem bark of X. strumarium exhibited a moderate-to-strong radical scavenging activity and ferric-reducing power and possessed a significant amount of TPCs and TFCs.

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Chemical Composition, Antioxidant, Alpha-glucosidase Inhibitory, Anticholinesterase and Photoprotective Activities of the Aerial Parts of Schinus molle L.

Bouhenna

Bensouici²,

Khattabi³

et al. 2021

CBC

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Background: Schinus molle L. is a medicinal and aromatic plant widely used in folk medicine commonly found in Algeria and Mediterranean region. In the present work, we investigated the in vitro antioxidant, anti-cholinesterase, αglucosidase inhibitory and photoprotective potentials of the Schinus molle L. plant’s extract/fractions. The metabolite profile of Schinus molle L. was analyzed using RP-HPLC and GC-MS. Methods: Antioxidant activity was assessed using different tests. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase and butyrylcholinesterase inhibitory activities. Antidiabetic activity was investigated by αglucosidase inhibition test, whereas photoprotective activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. The extracts were then submitted to RP-HPLC–PDA and GC–MS analysis. Results: Ethyl acetate and buthanol fractions with high contents of total phenolics and flavonoids exhibited the strongest antioxidant activity. Ethyl acetate extract exhibited considerable enzyme inhibition potential on acetylcholinesterase, butyrylcholinesterase and α-glucosidase with percentages of inhibition of 99.08 ± 0.79 %, 100 % and 98.80 ± 0.18 % respectively. Furthermore, the ethyl acetate fraction showed high photoprotective activity with the sun protection factor (SPF) value = 38,26 ± 0.73. Three phenolic acids (gallic, tannic and vanillic acids) and five flavonoids (myricetin, apigenin, naringenin, rutin and quercetin) were identified with RP-HPLC–PDA. Conclusion: These findings suggest that Schinus molle L. may be useful in the development of an alternative agent for oxidative stress, Alzheimer’s disease, diabetes and could be used as a natural sunscreen in pharmaceutics or cosmetic formulations.

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Antioxidant activity of extracts from Schinus molle L. and Gleditsia triacanthos L.

Cited by 3 publications

References 14 publications

Optimization of Polyphenols Recovery from Schinus molle L. Peel Using Response Surface Methodology (RSM)

Optimization of Polyphenols Recovery from Schinus molle L. Peel Using Response Surface Methodology (RSM)

Antioxidant activity of extracts from Xanthium strumarium – A medicinal plant from the Kingdom of Lesotho

Chemical Composition, Antioxidant, Alpha-glucosidase Inhibitory, Anticholinesterase and Photoprotective Activities of the Aerial Parts of Schinus molle L.

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