Antioxidant Activity of Endophytic Bacteria Derived from Hoya multiflora Blume Plant and Their Cellular Activities on Schizosaccharomyces pombe

Pudjas, Nabila Tsoerayya Gustia; Mubarik, Nisa Rachmania; Astuti, Rika Indri; Sudirman, Lisdar Idwan

doi:10.4308/hjb.29.2.214-221

Cited by 4 publications

(5 citation statements)

References 42 publications

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“…Phaenibacillus and Bacillus are endophytic bacteria found in the medicinal plant Eucalyptus pellita (Susanti et al 2021). Pudjas et al (2022) reported Bacillus as endophytic bacteria derived from Hoya multiflora Blume. produced antioxidant activity.…”

Section: A B Discussionmentioning

confidence: 99%

Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves

Arivo

MUBARIK²,

Rusmana³

et al. 2023

Biodiversitas

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Abstract. Arivo D, Mubarik NR, Rusmana I, Batubara I. 2023. Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves. Biodiversitas 24: 415-422. Girang (Leea indica) is one of the medicinal plants with various bioactive compounds that act as an antioxidant. However, isolating these bioactive compounds need a significant amount of biomass. Endophytic bacteria can be a good solution to produce the same bioactive compounds as their host. This study determined whether the potential isolate produces high antioxidant activity in various solvents, phytochemical screening, determination of total flavonoid and phenolic contents, and identification of the isolate. The BT4 isolate was isolated from L. indica leaves. The supernatant of BT4 was extracted in fractionated extraction. The antioxidant activity was determined using a DPPH assay. Folin-Ciocalteu and aluminum chloride colorimetric methods analyzed the total flavonoid and phenolic content. The isolate was identified using the 16S rRNA method. The best IC50 was obtained from n-butanol extract with a value of 59.021±0.541 ?g/mL. The flavonoid and phenolic contents from n-butanol extract have the value of 4.686±0.476 mgQE/g extract and 31.406±0.716 mgGAE/g extract, respectively. The BT4 isolate contained flavonoids, saponin, tannins, terpenoids, and steroids and was identical to Bacillus tequilensis with 99% similarity. This study revealed that endophytic bacteria from L. indica leaves could be a potential source of the novel natural antioxidant compound.

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Section: A B Discussionmentioning

confidence: 99%

Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves

Arivo

MUBARIK²,

Rusmana³

et al. 2023

Biodiversitas

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“…Two Bacillus strains ( Bacillus siamensis , Bacillus aryabhatii ) isolated from the Hoya multiflora Blume plant showed high phenolic contents of 26.17 ± 0.48 and 16.86 ± 0.59 mg/g of gallic acid equivalents (GAE), respectively. It correlates with the high antioxidant activities of bacterial crude extract [ 70 ]. Another endophytic Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Endophytic bacteria isolated from Urtica dioica L.- preliminary screening for enzyme and polyphenols production

Marchut-Mikołajczyk,

Chlebicz,

Kawecka

et al. 2023

Microb Cell Fact

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Endophytes, especially those isolated from herbal plants, may act as a reservoir of a variety of secondary metabolites exhibiting biological activity. Some endophytes express the ability to produce the same bioactive compounds as their plant hosts, making them a more sustainable industrial supply of these substances. Urtica dioica L. (common stinging nettle) is a synanthropic plant that is widely used in herbal medicine due to the diversity of bioactive chemicals it contains, e.g., polyphenols, which demonstrate anti-inflammatory, antioxidant, and anti-cancerous capabilities. This study aimed at isolating endophytic bacteria from stinging nettles for their bioactive compounds. The endophytic isolates were identified by both biochemical and molecular methods (16S rRNA) and investigated for enzymes, biosurfactants, and polyphenols production. Each of the isolated bacterial strains was capable of producing biosurfactants and polyphenols. However, three of the isolated endophytes, identified as two strains of Bacillus cereus and one strain of Bacillus mycoides, possessed the greatest capacity to produce biosurfactants and polyphenols. The derivatized extracts from culture liquid showed the 1.633 mol l−1 (9.691 mg l−1) concentration of polyphenol compounds. Therefore, the present study signifies that endophytic B. cereus and B. mycoides isolated from Urtica dioica L. could be a potential source of biosurfactants and polyphenols. However, further study is required to understand the mechanism of the process and achieve efficient polyphenol production by endophytic bacteria.

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“…The presence of carotenoid, phenolic and flavonoid indicated these compounds might play a role as antioxidants. The correlation of phenolic and flavonoid content with the antioxidant activity of an extract already reviewed (Sariwati et al 2019;Pudjas et al 2022). Phenolic and flavonoids are known as radical scavengers, metal chelators, reducing agents, and hydrogen donors.…”

Section: Discussionmentioning

confidence: 99%

“…The total phenolic and flavonoid compounds were measured following the method described by Pudjas et al (2022). The antioxidant assay of extracts were measured through DPPH assay, which previously described by Batubara et al (2009).…”

Section: Total Phenolic Total Flavonoid and Antioxidant Activity Assaymentioning

confidence: 99%

Antioxidant Properties of Active Fraction Extract Derived from Yellow-Red Pigment Produced by the Marine Sponge-Associated Bacterium Bacillus haikouensis AGS112 and Identification of Related Compounds

et al. 2023

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Sponge-associated bacteria can produce bioactive compounds similar to the host. Here, the investigation of antioxidant properties of the yellow-red pigment produced by sponge-associated bacterium AGS112 was conducted using in vitro and in vivo analysis. The 16S rRNA gene sequence showed the sponge-associated bacterium AGS112 has the closest similarity with Bacillus haikouensis C-89 (99%). The crude pigment extract produced by Bacillus haikouensis AGS112 contained both phenolic and flavonoid. The peaks occurred at 412 nm and 664 nm, which indicated as the presence of carotenoids and flavonoids, respectively. The active fraction extract obtained through bio-autography TLC, and had more potent antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) compared to the crude pigment extract with an IC50 value of 68.62±0.59 µg/ml and 198.88±1.66 µg/ml, respectively. Moreover, the active fraction extract at the concentration of 35 µg/ml could better enhance the viability of Schizosaccharomyces pombe and achieve the highest expression of sod1 and ctt1 genes. Metabolite profiling using LC-MS analysis confirmed the active fraction extract contains eudesmin and artelastin that might be contributed as antioxidants. These results suggest that the active fraction extract of the yellow-red pigment produced by B. haikouensis AGS112 had potency to be used as candidate for natural antioxidants.

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Antioxidant Activity of Endophytic Bacteria Derived from Hoya multiflora Blume Plant and Their Cellular Activities on Schizosaccharomyces pombe

Cited by 4 publications

References 42 publications

Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves

Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves

Endophytic bacteria isolated from Urtica dioica L.- preliminary screening for enzyme and polyphenols production

Antioxidant Properties of Active Fraction Extract Derived from Yellow-Red Pigment Produced by the Marine Sponge-Associated Bacterium Bacillus haikouensis AGS112 and Identification of Related Compounds

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