Antioxidant Activity of Coconut (Cocos nucifera L.) Protein Fractions

Abstract

Coconut cake is an abundant and good potential edible protein source. However, until now it has not been extensively used in the food industry. To promote its usage, the characterization, nutrition value and antioxidant activity of coconut cake protein fractions (albumin, globulin, prolamine, glutelin-1 and glutelin-2) were studied. Results revealed that all the albumin, globulin, glutelin-1 and glutelin-2 fractions showed a high nutrition value. The prolamine, glutelin-1 and glutelin-2 all exhibited good radical scavenging activity and reducing power, and the globulin and prolamine showed high ion chelating ability (89.14–80.38%). Moreover, all the fractions except glutelin-2 could effectively protect DNA against oxidative damage. Several peptides containing five to eight amino acids with antioxidant activity were also identified by LC-MS/MS from the globulin and glutelin-2 fractions. The results demonstrated that the coconut cake protein fractions have potential usages in functional foods.

Proteomic analysis of oilseed cake: a comparative study of species‐specific proteins and peptides extracted from ten seed species

“…Moreover, it is difficult to compare results, even within the seeds of one species, if the authors use different raw materials, extraction methods, and electrophoresis conditions. We obtained SDS‐PAGE profiles similar to those that have been described previously for coconut meal, 19,20 flaxseed meal, 21 hemp seed meal and hemp protein isolate, 22,23 milk thistle seed protein isolate, 24 black cumin flour obtained from Nigella damascena , 25 pumpkin seed protein extract, 11,26 rapeseed meal, 27–29 sesame protein isolate and seed flour, 30,31 and finally, from sunflower flour and protein concentrate 32,33 …”

In vitro and in silico analysis of the effect of fluconazole, an antifungal drug, on DNA

“…28 Considering the mentioned information, in this study, the concentration of FeSO4 and H2O2 was selected as 1.5 afnd 6 mM as used by Li and her coworkers. 29 Fluconazole concentration ≥ 88 μM could protect 46 µM of DNA against radical hydroxyl produced by the reaction between 1.5 mM of FeSO4 and 6 mM of H2O2 while the drug concentration ≤ 44 μM could not provide the protection. Therefore in our opinion, the protection was provided by binding and covering DNA by the drug.…”

Effect of Bezafibrate on Deoxyribonucleic Acid in the Presence of Iron and Copper

“…To examine the protective effects of bezafibrate on DNA against the radical hydroxyl, the pUC19 plasmid DNA (49 µM bp) was treated with 60 µM CuCl2 plus 6mM H2O2 plus different concentrations of bezafibrate (2.6, 1.3, 0.13, Türk Diyab Obez / Turk J Diab Obes / 2019; 3: 131-136 0.013, and 0.0013 mM) and followed by incubation at 37°C for 1 hour. After that, the reaction mixture was subjected to analysis on agarose gel (1%) electrophoresis for 55 minutes at 90 volts (20).…”