Antioxidant Activity of Alkaloid Fractions of Zanthoxylum Acanthopodium Dc. Fruits With 1,1-Diphenyl-2-Picrylhydrazyl Assay

Rosidah, Rosidah; Hasibuan, Poppy Anjelisa Zaitun; Haro, Ginda; Masri, Puteri; Satria, Denny

doi:10.22159/ajpcr.2018.v11s1.26560

Cited by 9 publications

(6 citation statements)

References 14 publications

(14 reference statements)

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“…The reports has shown that alkaloids extracts of Euphorbia granulate leaf was shown a great antiradical activity by the characterization of several substances which inhibit free radical DPPH [22]. Further, Rosidah et al, [26] have reported that alkaloid fractions of Z. acanthopodium DC. fruit have very strong antioxidant potential by DPPH method.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Alkaloid Extract of Cucumis Trigonus Roxb

Pratima

2019

Int J Pharm Pharm Sci

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Objective: To evaluate the antioxidant and antibacterial efficiency of alkaloids of root, leaf, and fruit of Cucumis trigonus. Methods: The antioxidant and antibacterial properties of alkaloid extracts were assessed by ferric thiocynate (FTC), 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, hydrogen peroxide radical scavenging assay, superoxide radical scavenging activity and ferric reducing power were analyzed separately for its inhibition percentage at different concentration (20µg, 30µg, 40µg and 50µg/ml) and antibacterial activity by agar disc diffusion and minimum inhibitory concentration (MIC) against Staphylococcus aureus (MTCC Code-9886), Pseudomonas aeruginosa (MTCC Code-6458). Results: The highest percentage of extraction yield was observed in fruit followed by leaf and root. The maximum content of alkaloid was found in fruit and root compared to leaf. The scavenging efficiencies of the extracts increased with the increasing concentrations. Appreciable levels of total antioxidant activity by FTC (2.112±0.011%), DPPH radical scavenging activity (1.912±0.001%), superoxide radical scavenging activity (0.955±0.021%) and hydrogen peroxide radical scavenging activity (0.914±0.087 %) were observed at 50µg/ml of fruit extract. However, ferric reducing power was more in the root (0.893±0.0870%) compared to fruit (0.791±0.023%) and leaf (0.520±0.00 %) at 50µg/ml. The alkaloid extracts of root and fruit exhibited more antibacterial activity against Staphylococcus aureus (16.51±0.05 and 19.68±0.03 mm) and Pseudomonas aeruginosa (12.2±0.03 and14.2±0.06) at 100µg/ml than leaf. The minimum inhibitory concentration (MIC) of the root, leaf, and fruit was in the range of 3.125µg/ml to 35µg/ml for the pathogenic bacteria. Conclusion: Data from the present results revealed that the alkaloid extracts of fruit and root of C. trigonus show good antioxidant and antibacterial potential than leaf. Hence, may be explored for the formation of new antibacterial with antioxidant drugs.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Alkaloid Extract of Cucumis Trigonus Roxb

Pratima

2019

Int J Pharm Pharm Sci

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“…(1 kg) were repeatedly macerated with ethanol 96% (3 x 3 d, 7.5 L), The filtrate was evaporated to give a viscous extract. Viscous extract was fractionated with n-hexane and continue with chloroform at pH 3,7 and 9 [10], [11], [12].…”

Section: Methodsmentioning

confidence: 99%

The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

Dalimunthe¹,

Hasibuan²,

Satria³

2019

Open Access Maced J Med Sci

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AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro. MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and 25 µg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULT: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes. CONCLUSION: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes.

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“…The total phenol concentration (TPC) of the sample was determined using a Folin ciocalteu technique with gallic acid as a standard [15,16,18].…”

Section: Determination Of Total Phenol Concentrationmentioning

confidence: 99%

Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

et al. 2021

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Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

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Antioxidant Activity of Alkaloid Fractions of Zanthoxylum Acanthopodium Dc. Fruits With 1,1-Diphenyl-2-Picrylhydrazyl Assay

Cited by 9 publications

References 14 publications

Antioxidant and Antibacterial Activity of Alkaloid Extract of Cucumis Trigonus Roxb

Antioxidant and Antibacterial Activity of Alkaloid Extract of Cucumis Trigonus Roxb

The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

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