Antioxidant Activity of Alkaloid Compounds from Litsea cubeba Lour

Dalimunthe, Aminah; Hasibuan, Poppy Anjelisa Zaitun; Silalahi, Jansen; Sinaga, Siti Fatimah; Satria, Denny

doi:10.13005/ojc/340270

Cited by 25 publications

(24 citation statements)

References 13 publications

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“…Diabetes is a state of increased free radical production that elicits oxidative stress as a consequence of an imbalance between radicalgenerating and radical-scavenging systems [3]. The uncontrolled production of oxygen free radicals and the unrateable system of antioxidant capability in protection results in the cause of many diseases, such as cancer, diabetes, heart diseases, Alzheimer's, and ageing [23]. Higher levels of HbA1c cause complications.…”

Section: Introductionmentioning

confidence: 99%

Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

Surbakti¹,

Sitorus²,

Rosidah³

et al. 2019

Open Access Maced J Med Sci

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AIM: This study aimed to investigate Saurauia vulcani Korth. leaves. the activity of ethanol extract in hypoglycemic, superoxide dismutase (SOD), glycosylated haemoglobin (HbA1c) and detection of insulin expression by immunochemistry METHODS: Saurauia vulcani Korth. Leaves powder was extracted by maceration method with ethanol 96%. The extract was administrated orally in doses of 100 mg/kg BW for 27 days. Diabetes was induced in rats by administered of Nicotinamide (NA) 230 mg/kg BW and streptozotocin (STZ) 65 mg/kg BW. Level of blood glucose, SOD, HbA1c were measured, and histopathology pancreas was observed to determine insulin expression with immunochemistry RESULTS: Ethanol extract of Saurauia vulcani Korth. Leaves (EESL) shown a significantly (*p < 0.05) reduced in blood glucose levels at 104.25 ± 2.562 mg/dL and HbA1c level at 32.53 ± 0.188 ng/mL, but increased SOD level at 60.64 ± 0.740 pg/mL and histopathology study shown secretion insulin as seen number of expressions of insulin / slice 31.00 ± 0.315. CONCLUSION: The result of this study showed EESL possess the hypoglycemic activity and increase the level of SOD but decrease the level of HbA1c in diabetic rat condition. The mechanism of the activity is suggested by stimulating the insulin secretion of pancreas β-cells which were damaged.

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Section: Introductionmentioning

confidence: 99%

Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

Surbakti¹,

Sitorus²,

Rosidah³

et al. 2019

Open Access Maced J Med Sci

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“…However, these fractions demonstrated lower ABTS scavenging ability than the control, vitamin C (IC50 = 13.50 µg/mL). Dalimunthe et al 24 reported higher IC50 values for alkaloid fractions of L. cubeba. Similarly, H2O2 scavenging activity also increased in a dosedependent manner (Figure 2a), with the stem alkaloid fraction scavenging 55.66% and 80.42% of H2O2 at 20 µg/mL and 100 µg/mL, respectively.…”

Section: Resultsmentioning

confidence: 95%

“…Antioxidant activity Numerous findings agree that diseases such as cancer, diabetes, cardiovascular and neurodegenerative disorders are exacerbated by oxidative stress arising from the inability of the body to completely scavenge ROS, which damage biological molecules including lipids, proteins and deoxyribonucleic acid (DNA). [19][20][21][22][23][24] Despite the availability of synthetic antioxidants such as butylated hydroxy anisole (BHA) and butylated hydroxy toluene (BHT), there is increased search for natural antioxidants as these are considered to be safer, cheaper and less toxic. The antioxidant activity of alkaloid fractions of T. hirtum was evaluated by various in-vitro models including the DPPH, ABTS, H2O2 radical scavenging activity, reducing power and metal chelating activity.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Alkaloid Fractions of Tristemma hirtum P. Beauv

Ita¹,

Eduok²

2020

TJNPR

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A wide range of ailments such as microbial infections and diseases associated with increased reactive oxygen species (ROS) in the body including inflammation, cardiovascular diseases, immune system dysfunction, arteriosclerosis, diabetes and neurogenerative diseases are being treated with medicinal plants in many cultures around the world. 1-2 Its use is very popular in Africa where the dependence on traditional medicine practice is still high. Despite the presence of synthetic drugs that can treat microbial infections and retard ROS, challenges such as the high cost, microbial resistance and toxicity have negatively impacted on the use of these synthetic drugs for effective health care delivery, resulting in treatment failures. This has prompted the renewed search for therapeutic alternatives from plant sources, which are considered to be safe, cost effective and affordable. 1 T. hirtum, family Melastomataceae (local names: Orunchi, Igbo; Udia inuen, Ibibio; Apiko, Yoruba) is a medicinal plant that grows abundantly in Nigeria and in many West African countries. The leaves are used in traditional medicine to treat skin diseases, headache, menstrual pain and for blood purification. Limited biological activity has been reported for this plant. For instance, a decoction of the leaf extract showed antimicrobial, antidermatophytic

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“…The samples were analyzed using a FACScan flow cytometer. On the basis of DNA content, the percentage of cells in each stage in the cell cycle (G 1 , S, and G 2 /M) was calculated using ModFit Lt. 3.0 s [ 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

The anticancer activities of Vernonia amygdalina Delile. Leaves on 4T1 breast cancer cells through phosphoinositide 3-kinase (PI3K) pathway

Hasibuan

Harahap

Sitorus

et al. 2020

Heliyon

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Vernonia amygdalina Delile ( Asteraceae ) is used in traditional medicine to treat diabetes mellitus, and some research provides its activity to treat breast cancer. The aim of this study is to assess the anticancer activity of Vernonia amygdalina Delile leaves fractions on 4T1 breast cancer cells. Analysis of phytochemical compounds were carried out with LC-MS/MS. Cytotoxic activity was determined using the MTT method in the 4T1 cell line. Apoptosis, the cell cycle, and PI3K and mTOR profiles were analyzed with flow cytometry. The phytochemicals found were diterpene (ingenol-3-angelate) and some phenolics (chlorogenic acid and 4-methoxycinnamic acid), flavonoids (apigetrin, apigenin, luteolin, diosmetin, baicalin, rhoifolin, and scutellarin), and coumarines (7-hydroxycoumarine, 4-methylumbelliferone, and 4-methylumbelliferyl glucuronide). The results of the MTT assay showed that the IC 50 values n -hexane fraction, ethylacetate fraction (EAF), and ethanol fractions were 1,860.54 ± 93.11, 25.04 ± 0.36, and 1,940.84 ± 96.37 μg/mL, respectively. EAF induced early and late apoptosis, inhibited cell cycle progression on the G 2 /M phase, and inhibited PI3K and mTOR expression. The EAF of Vernonia amygdalina Delile leaves showed anticancer activity on 4T1 breast cancer cells through induction of apoptosis, enhanced cell accumulation on G 2 /M phases in the cell cycle, and inhibited expression of PI3K and mTOR.

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Antioxidant Activity of Alkaloid Compounds from Litsea cubeba Lour

Cited by 25 publications

References 13 publications

Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

Antioxidant and Antibacterial Activity of Alkaloid Fractions of Tristemma hirtum P. Beauv

The anticancer activities of Vernonia amygdalina Delile. Leaves on 4T1 breast cancer cells through phosphoinositide 3-kinase (PI3K) pathway

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