Antioxidant Activity and Vitamin C Concentration Analysis of Gandaria (Bouae macrophylla Griff) Ethanol Extract Using Spectrophotometry UV Vis

Abstract

Antioxidants are chemical compounds that donate one or more electrons to free radicals to inhibit free radical reactions. One of the potential antioxidant sources is gandaria fruit (Bouea Macrophylla Griff). This research aims to determine the antioxidant activity and vitamin C content of the gandaria fruit extract. Gandaria fruit was macerated with ethanol as a solvent. Furthermore, the rotated extract was carried out for the phytochemical screening. The results of the phytochemical screening of the gandaria fruit extract showed a positive of flavonoids, terpenoids, tannins, saponins, and phenolics. The gandaria fruit extract was tested for antioxidants and quantitative analysis of vitamin C levels to obtain IC50 values and vitamin C levels using spectrophotometry UV-Vis at a wavelength of 517nm with vitamin C as a positive control. The results of spectrophotometric measurements revealed that the gandaria fruit extract had an IC50 value of 5.72 g/ml, and vitamin C had an IC50 value of 2.260 g/ml, indicating that the gandaria fruit extract and vitamin C had very strong antioxidants. The value of vitamin C levels was 0.526 mg.

Formulation and Antioxidant Activity Analysis of Jotang Herb (Acmella paniculata) Extract Mask Cream

“…18 In this study, the antioxidant activity test was carried out on 3 cream mask preparation formulas from the ethanol extract of jotang herbs in each formula, with the aim of knowing the IC 50 value in each formula for the preparation of jotang herb extract cream masks with extract ratios as much as 1 gram and 10 grams and to find out the comparison of mugwort cream masks. Measurement of the absorbance of cream mask samples was carried out with various concentrations (2,4,8,16, and 32 ppm) and then measured at a wavelength of 517 nm. The results of the IC50 value of the antioxidant test for cream mask preparations from the ethanol extract of the jotang herb (Acmella paniculata) showed that Formula 0 cream masks had very strong antioxidants (40.1959 µg/ml) and Formula I had very strong antioxidant value (25.663 µg/ml).…”

“…Antioxidant compounds are needed for the body to protect against exposure to free radicals, which work by donating electrons to compounds that have oxidant properties so that they easily inhibit oxidant activity. 2 One of the plants that contain antioxidants and is often used is the jotang plant (Acmella paniculata), which has an IC50 value of 1.77 µg/ml. It is a herb that grows wild in gardens, along rice fields, river banks, and moist soils in tropical and subtropical regions.…”