Antioxidant activity and stability of α‐tocopherol, resveratrol and epigallocatechin‐3‐gallate in mixture and complexation with bovine serum albumin

Abstract

Intermolecular interactions among α‐tocopherol, resveratrol and EGCG appear to be antagonistic based on antioxidant activity but improve their structural stability and preserve antioxidant property. These bioactive compounds interact with BSA to form tri‐ligand complexes. Such tri‐ligand complexes maintain structural integrity and antioxidant property of a‐tocopherol, resveratrol and EGCG simultaneously and to a greater degree than is observed for mono‐ligand complexes with BSA.

Improving Physicochemical Stability of Quercetin-Loaded Hollow Zein Particles with Chitosan/Pectin Complex Coating

“…ABTS and DPPH assays of quercetin-loaded HZ and HZ-chi/pec particles were estimated according to the method of Dong et al and Pan et al [ 24 , 25 ]. In brief, equal volumes of potassium persulfate (2.6 mM) and ABTS (7.4 mM) were mixed and allowed to react and generate ABTS + for 12 h in the dark.…”

Effect of resveratrol and curcumin on formation of N‐Carboxymethyl lysine and its intracellular oxidative stress

“…The formation of AGEs in the presence of Res and α‐dicarbonyls (GO and MGO) was inhibited while protein carbonylation and thiols depletion were significantly promoted as compared to the GO and MGO systems alone (Arcanjo et al ., 2018). BSA interacts with, Res, α‐tocopherol and epigallocatechin‐3‐gallate to form multi‐ligand complexes, which reduces its masking effect on antioxidant activity of these compounds (Dong et al ., 2020). Concerning CU‐containing samples (240 µmol L −1 ), after heating for 20 min, the CML content was only 12 ± 4 µg mL −1 (Fig.…”

Elaboration of Cationic Soluble Soybean Polysaccharides-Epigallocatechin Gallate Nanoparticles with Sustained Antioxidant and Antimicrobial Activities

“…ABTS assays were performed according to Dong et al A 7 mM ABTS aqueous solution and a 2.5 mM potassium persulfate (K 2 S 2 O 8 ) aqueous solution were mixed in a brown flask and reacted at 25 °C for 16 h under dark conditions. The ABTS + radical working solution was acquired by diluting the ABTS + solution with 10 mM PBS (pH 7.4) to an absorbance of 0.70 ± 0.02 at 734 nm and was placed at room temperature (25 °C).…”