Antioxidant activity and anticancer effect of<i>Vitex agnus-castus</i>L. (Verbenaceae) seed extracts on MCF–7 breast cancer cells

Aslantürk, Özlem Sultan; Çelik, Tülay Aşkın

doi:10.1080/00087114.2013.850797

Cited by 7 publications

(7 citation statements)

References 48 publications

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“…Viable cells will have a clear cytoplasm, whereas dead cells will have a blue cytoplasm [14,15]. Number of viable and/or dead cells per unit volume is determined by light microscopy as a percentage of untreated control cells [15,16].…”

Section: Trypan Blue Dye Exclusion Assaymentioning

confidence: 99%

In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages

Aslantürk¹

2018

Genotoxicity - A Predictable Risk to Our Actual World

271

241

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Cytotoxicity is one of the most important indicators for biological evaluation in vitro studies. In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. In order to determine the cell death caused by these damages, there is a need for cheap, reliable and reproducible short-term cytotoxicity and cell viability assays. Cytotoxicity and cell viability assays are based on various cell functions. A broad spectrum of cytotoxicity assays is currently used in the fields of toxicology and pharmacology. There are different classifications for these assays: (i) dye exclusion assays; (ii) colorimetric assays; (iii) fluorometric assays; and (iv) luminometric assays. Choosing the appropriate method among these assays is important for obtaining accurate and reliable results. When selecting the cytotoxicity and cell viability assays to be used in the study, different parameters have to be considered such as the availability in the laboratory where the study is to be performed, test compounds, detection mechanism, specificity, and sensitivity. In this chapter, information will be given about in vitro cytotoxicity and viability assays, these assays will be classified and their advantages and disadvantages will be emphasized. The aim of this chapter is to guide the researcher interested in this subject to select the appropriate assay for their study.

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Section: Trypan Blue Dye Exclusion Assaymentioning

confidence: 99%

In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages

Aslantürk¹

2018

Genotoxicity - A Predictable Risk to Our Actual World

271

241

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“…Çalışmadan elde edilen sonuçlara göre, metanol ekstresinde bulunan fenolik madde içeriğinin, ekstrenin gösterdiği antioksidan aktiviteyi de etkilediği söylenebilir. Bitkilerden farklı yöntemlerle elde edilen ekstrelerin çözücü özelliklerine göre farklı bileşikler içermesi, ekstrelerin DPPH üzerinden serbest radikali süpürücü aktivitelerinin birbirinden farklı olmasına ve polar çözücüler kullanılarak elde edilen ekstrelerin, polar olmayan çözücüler kullanılarak elde edilen ekstrelerden çok daha yüksek DPPH radikal süpürücü aktivite göstermesine neden olabilmektedir [27][28][29][30]. Tek bir yöntemle antioksidan aktivitesi hakkında karar vermenin doğru bir yaklaşım olmadığı, antioksidan aktivitesi belirlenirken, farklı yöntemler kullanılması ve elde edilen aktivite sonuçlarının, her bir özelliğe göre verilmesinin daha doğru bir yaklaşım olacağı da düşünülmektedir.…”

Section: Discussionunclassified

“…Özellikle MCF-7 hücrelerinde ortaya çıkan güçlü sitotoksik ve apoptotik etki, metanol ekstresinde bulunan fenolik bileşikler (fenoller, taninler ve antrakinonlar) ile ilişkilendirilebilir. Çünkü fenolik bileşikler antioksidan aktivitenin yanı sıra, karsinojen metabolizmasının modülasyonu, hücre çoğalması ve farklılaşmasında onkogenler ve tümör baskılayıcı genlerde gen ifadesinin düzenlenmesi, hücre döngüsünün durdurulması ve apoptozisin teşvik edilmesi, çeşitli sinyal akış yolaklarının inhibe edilmesi gibi çok sayıda tamamlayıcı ve bir arada çalışabilen etki mekanizmasına sahiptirler [28][29][30][31][32][33][34][35][36][37][38][39][40][41][42]. Ayrıca, fenolik bileşiklerin konsantrasyona bağlı olarak DNA'yı inhibe etmek suretiyle sitotoksik etki gösterdikleri de bilinmektedir [43].…”

Section: Martins Ve Arkadaşlarıunclassified

Carpobrotus acınaiformis L. Metanol Ekstresinin Fitokimyasal Taraması ve Ekstrenin İnsan Metastatik Meme Kanseri (MCF-7) ve İnsan Kolon Kanseri (Caco-2) Hücreleri Üzerindeki Sitotoksik ve Apoptotik Etkisinin Araştırılması

Çelik

Aslantürk

2018

Marmara Fen Bilimleri Dergisi

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ÖzBu çalışmanın amacı, C. acinaciformis L. (makas otu) bitkisinin toprak üstü kısımlarından elde edilen metanol ekstresinde (ME) fitokimyasal madde taramasının yapılması, ekstrenin toplam fenolik madde miktarı, antioksidan aktivitesi ve in vitro sitotoksik ve apoptotik etkilerinin araştırılmasıdır. Ekstrede bulunan fitokimyasallar standart metod ve HPLC analizi kullanılarak belirlenmiştir. C. acinaciformis ME, antioksidan aktivitesi (DPPH radikalini süpürme, H 2 O 2 süpürme ve metal şelatlama assay), methanol ekstresinin MCF-7 ve Caco-2 hücreleri üzerindeki in vitro sitotoksik etkisi MTT assay ile apoptotik etkisi ise DNA difüzyon assay ile belirlenmiştir.Yapılan kalitatif fitokimyasal tarama test sonuçlarına göre; C. acinaciformiş ME'de fenoller, taninler, flavonoidler ve antrakinon'ların bulunduğu, alkaloid ve saponinlerin ise bulunmadığı belirlenmiştir. Ekstrenin toplam fenolik madde miktarı 61.26 ± 0,110 mg GAE/g ekstre olarak hesaplanmıştır. ME'nin denenen bütün konsantrasyonlarda (100 μg/ml, 250 μg/ml, 500 μg/ml ve 1000 μg/ml) yüksek seviyede DPPH radikalini süpürme aktivitesine sahip olduğu H 2 O 2 süpürme aktivitesinin çok düşük olduğu, metal şelatlama aktivitesinin ise bulunmadığı belirlenmiştir. ME, MCF-7 hücreleri üzerinde oldukça yüksek oranda sitotoksik etki göstermiş (p<0.05), Caco-2 hücreleri üzerindeki sitotoksik etkisi ise daha düşük oranda olmuştur. ME'nin MCF-7 ve Caco-2 hücreleri üzerinde oluşturduğu apoptotik etki de sitotoksik etkiye benzerlik göstermiştir. Metanol ekstresinin MCF-7 hücreleri üzerindeki apoptotik etkisi, Caco-2 hücrelerine nazaran daha fazla olmuş ve aradaki farkın istatistiki açıdan da önemli olduğu bulunmuştur (p<0.05). AbstractMedicinal plants constitute an integral part of traditional healing customs in tradiotional culture. Many plants synthesize substances that are useful to the maintenance of health in humans and other animals. The demand for medicinal plants is currently increasing in both developed and developing countries for various reasons. For some it would be the growing recognition that natural products have fewer or even no side effects; for others it would be their accessibility and affordable costs that would tip the scales. A large number of extracts of plants, as well as some pure compounds isolated from these plants, have been screened for cytotoxicity against a wide range of cell lines. Such studies are necessary to determine whether biological activity is a specific phenomenon or whether it should be merely attributed to nonspecific toxicity. However, scientific data on biological effects and mechanisms of action of extracts from medical plants are still insufficient.

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“…The cell line studies have mostly concentrated on DNA damage, cytotoxicity through apoptosis and free radical scavenging activitiy. 11 The presence of mammary tumor (benign or malignant) was histopathologically confirmed. Antitumor activity of methanolic extract of seeds of Vitex agnus has been evaluated by treating the tumor induced animals for 15 days.…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitex agnus Extract on MNU Induced Mammary Tumor of Sprague Dawley Rats

Kour¹,

Anantharaya

Bhat³

et al. 2017

JYP

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INTRODUCTIONBreast cancer is one of the most common cancers with over one million cases reported worldwide. It accounts up to 30% of all the cancers reported in female's worldwide. 1 Regardless of having advanced treatments, the disease continues to have the highest rate of treatment failure due to tumor resistance, both intrinsic and acquired.2 Many treatment strategies have been applied for the best results to ameliorate the cancer, but none has shown any significant results so far. Natural products have long been an important source for the treatment of different types of cancer. More than a thousand plants have been found to possess significant anticancer properties.3 Over 60% of currently used anticancer agents are derived from natural sources. 4 The Vitex agnus castus (VAC) is a shrub found in central Europe and central Asia has brown or black colored pepper like seeds having significant levels of herbal and medicinal properties.5 It is a well-known herb used for the treatment of female reproductive problem, especially pre-menstrual syndrome and certain endocrine related problems. 6 Studies reported that the fruit of this plant contains phenolic compounds like terpenes, flavonoids and aetheric oils, etc.7 Further studies on it showed that ethanolic extract from dried ripe VAC fruits displays cytotoxic activity against six human cell lines from different cancer tissue origins.8 Studies for antioxidant activity in the past have shown that decreased GSH levels are indicator of cell viability along-with increased MDA levels showing marked cell proliferation in tumor tissues whereas decreased levels of the same are indicator improved oxidative status of the tissue. From the recent literature, it is evident that ethanolic fruit extract of the VAC has shown some anticancer effects in, in-vitro studies and there is no recent data suggesting the anticancer property in vivo. Therefore, this study was conducted to elucidate the anticancer effect of the Vitex agnus seed extract along-with its antioxidant activity by estimating GSH and MDA levels on induced mammary tumor of SD rats in an in-vivo study. MATERIALS AND METHODS AnimalsAfter obtaining the permission from the institutional ethical committee, healthy, 30 days old inbred Sprague Dawley female rats of body weight 70-80 grams were taken for this study. Animals were maintained according to prescribed guidelines of a committee for control and supervision of experts on animals (CPCSEA), Govt. Of India, for the use of laboratory animals. All animals were maintained under normal day-night environment in temperature controlled institutional animal house. Animals were housed in polypropylene cages; paddy husk was used for bedding. Animals were given water ad libitum and standard rat feed pellet was used for feeding. Induction of tumor Preparation of the carcinogenMammary tumor was induced by injecting N-Methyl-N-Nitrosourea dissolved in normal saline with pH-4 maintained by adding 3% glacial acetic acid. The SD rats were given a single intraperitoneal dose of Effect o...

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Antioxidant activity and anticancer effect ofVitex agnus-castusL. (Verbenaceae) seed extracts on MCF–7 breast cancer cells

Cited by 7 publications

References 48 publications

In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages

In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages

Carpobrotus acınaiformis L. Metanol Ekstresinin Fitokimyasal Taraması ve Ekstrenin İnsan Metastatik Meme Kanseri (MCF-7) ve İnsan Kolon Kanseri (Caco-2) Hücreleri Üzerindeki Sitotoksik ve Apoptotik Etkisinin Araştırılması

Effect of Vitex agnus Extract on MNU Induced Mammary Tumor of Sprague Dawley Rats

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