Antioxidant Activity and Acteoside Analysis of Abeliophyllum distichum

Abstract

This study determined acteoside and its content in Abeliophyllum distichum via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (•OH), and O2− radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, •OH, and O2− radical scavenging activities of A. distichum leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in A. distichum leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in A. distichum leaves, which resulted in DPPH, •OH, and O2− radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that A. distichum leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.

UPLC-ESI-Q-TOF-MS-Based Metabolite Profiling, Antioxidant and Anti-Inflammatory Properties of Different Organ Extracts of Abeliophyllum distichum

“…In addition, AB displayed a stronger ability to decrease DPPH free radicals (RC 50 = 294.5 ± 16.4 µg/mL, Figure 1 C), ABTS cation radicals (RC 50 = 36.0 ± 0.63 µg/mL, Figure 1 D), and hydroxyl radical scavenging activity (86 ± 0.97%) than other organ extracts, suggesting that A. distichum branches possess a stronger antioxidant activity than that of the other analyzed organs. Recently, it has been shown that antioxidant activity of A. distichum leaf EtOH extracts is slightly higher than A. distichum stem EtOH extracts [ 15 ]. The variation of antioxidant activity might be mediated by phytochemical compositions and concentrations, which are influenced by season, climatic conditions, stages of maturity, and extraction conditions.…”

“…The absorbance was measured at 532 nm, after incubation for 30 min at 90 °C. Hydroxyl radical scavenging activity was calculated as described by Lee et al [ 15 ].…”

“…distichum leaf EtOH extract has a higher free radical scavenging activity than its stem extract, suggesting variation in biological activities in different organs of A . distichum [ 15 ]. Among phytochemicals in A. distichum , phenolic and phenylethanoid glycosides have been reported as multiple active compounds with therapeutic value [ 11 , 14 ].…”

“…Among phytochemicals in A. distichum , phenolic and phenylethanoid glycosides have been reported as multiple active compounds with therapeutic value [ 11 , 14 ]. For example, acteoside (phenylethanoid glycoside) and isoacteoside (dihydroxypheynylethyl glycoside) identified in leaves of A. distichum , exhibited antioxidant and anti-inflammatory activities, respectively [ 15 , 16 ], indicating that A. distichum could have functional applications in the cosmetic, biomedical, pharmaceutical, and other industries. While various studies have characterized the phenolic and phenylethanoid glycoside compounds from A. distichum , systematic investigation including the comparison of the metabolite composition in different organ and molecular mechanism underlying the anti-inflammatory effects is extremely limited.…”

Phytochemical Investigation and Biological Activities of Lantana rhodesiensis

“…The major phenolic compounds were identified in each extract ( Figure 3 ) based on their retention index and their PDA spectrum with comparison to a library. Results showed that all extracts were characterized by high quantities of isomers of acteoside, a phenolic molecule well known for its wide range of biological properties including anti-inflammatory, antioxidant and hepatoprotective activities [ 51 , 52 , 53 , 54 , 55 ].…”