Antioxidant Activities of Perilla frutescens against Low-Density Lipoprotein Oxidation in Vitro and in Human Subjects

Saita, Emi; Kishimoto, Yoshimi; Tani, Makoto; Iizuka, Maki; Toyozaki, M; Sugihara, Norie; Kondo, Kazuo

doi:10.5650/jos.61.113

Cited by 47 publications

(27 citation statements)

References 35 publications

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“…Catalase and SOD play important roles in the control of oxidative stress and apoptosis in Jurkat cells [35]. Similar to our findings, an aqueous extract of another rosmarinic acid-containing Lamiaceae herb, Perilla frutescens , was shown to upregulate the mRNA and protein expression of these antioxidant enzymes in cultured human vein endothelial cells [36]. …”

Section: Discussionsupporting

confidence: 81%

Rosmarinic Acid-Rich Extracts of Summer Savory (Satureja hortensisL.) Protect Jurkat T Cells against Oxidative Stress

Chkhikvishvili

Sanikidze

Gogia

et al. 2013

Oxidative Medicine and Cellular Longevity

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Summer savory (Satureja hortensis L., Lamiaceae) is used in several regions of the world as a spice and folk medicine. Anti-inflammatory and cytoprotective effects of S. hortensis and of its rosmarinic acid-rich phenolic fraction have been demonstrated in animal trials. However, previous studies of rosmarinic acid in cell models have yielded controversial results. In this study, we investigated the effects of summer savory extracts on H2O2-challenged human lymphoblastoid Jurkat T cells. LC-MS analysis confirmed the presence of rosmarinic acid and flavonoids such as hesperidin and naringin in the phenolic fraction. Adding 25 or 50 µM of H2O2 to the cell culture caused oxidative stress, manifested as generation of superoxide and peroxyl radicals, reduced cell viability, G0/G1 arrest, and enhanced apoptosis. This stress was significantly alleviated by the ethanolic and aqueous extracts of S. hortensis and by the partially purified rosmarinic acid fraction. The application of an aqueous S. hortensis extract doubled the activity of catalase and superoxide dismutase in the cells. The production of IL-2 and IL-10 interleukins was stimulated by H2O2 and was further enhanced by the addition of the S. hortensis extract or rosmarinic acid fraction. The H2O2-challenged Jurkat cells may serve as a model for investigating cellular mechanisms of cytoprotective phytonutrient effects.

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Section: Discussionsupporting

confidence: 81%

Rosmarinic Acid-Rich Extracts of Summer Savory (Satureja hortensisL.) Protect Jurkat T Cells against Oxidative Stress

Chkhikvishvili

Sanikidze

Gogia

et al. 2013

Oxidative Medicine and Cellular Longevity

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“…Phenolic compounds, major secondary metabolites in many edible plants (Barros et al, 2011;Kim et al, 2013), are of great importance for medical treatment. Seeds of Perilla frutescens are an essential Chinese herbal medicine and contain many bioactive phenolic compounds showing excellent anti-oxidative (Yamamoto and Ogawa, 2002;Zhao et al, 2010;Izumi et al, 2012), anti-biotic (Saita et al, 2012;Zhao et al, 2012a;Yang et al, 2013) and hepatic protection (for rosmarinic acid and caffeic acid; Yang et al, 2013) activities. In addition, luteolin and apigenin in perilla seed could improve several hypermonoaminergic neuropsychological disorders (Zhao et al, 2010(Zhao et al, , 2012a.…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantitation of Phenolic Compounds from the Seed and Pomace of Perilla frutescens Using HPLC/PDA and HPLC-ESI/QTOF/MS/MS

Zheng

Lin

et al. 2014

Phytochem. Anal.

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“…P. frutescens var. acuta (PF), a widely cultivated and consumed leafy vegetable, belongs to the Labiatae, and is used as a traditional medicine in Korea, Japan, China, and other Asian countries (Saita et al, 2012). There are two major types of PF, P. frutescens var.…”

mentioning

confidence: 99%

Barrier protective effects of rosmarinic acid on HMGB1‐induced inflammatory responses in vitro and in vivo

Yang

Lee

et al. 2013

Journal Cellular Physiology

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High mobility group box 1 (HMGB1) protein is a crucial cytokine that mediates response to infection, injury, and inflammation. Rosmarinic acid (RA) is an important component of the leaves of Perilla frutescens and has neuroprotective, anti-microbial, anti-oxidant, and anti-cancer effects but little is known of its effects on HMGB1-mediated inflammatory response. Here, we investigated this issue by monitoring the effects of RA on the lipopolysaccharide (LPS) or cecal ligation and puncture (CLP)-mediated release of HMGB1 and HMGB1-mediated modulation of inflammatory responses. RA potently inhibited the release of HMGB1 and down-regulated HMGB1-dependent inflammatory responses in human endothelial cells. RA also inhibited HMGB1-mediated hyperpermeability and leukocyte migration in mice. Furthermore, RA reduced CLP-induced HMGB1 release and sepsis-related mortality. Given these results, RA should be viewed as a candidate therapeutic agent for the treatment of various inflammatory diseases via inhibition of the HMGB1 signaling pathway.

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Antioxidant Activities of Perilla frutescens against Low-Density Lipoprotein Oxidation in Vitro and in Human Subjects

Cited by 47 publications

References 35 publications

Rosmarinic Acid-Rich Extracts of Summer Savory (Satureja hortensisL.) Protect Jurkat T Cells against Oxidative Stress

Rosmarinic Acid-Rich Extracts of Summer Savory (Satureja hortensisL.) Protect Jurkat T Cells against Oxidative Stress

Identification and Quantitation of Phenolic Compounds from the Seed and Pomace of Perilla frutescens Using HPLC/PDA and HPLC-ESI/QTOF/MS/MS

Barrier protective effects of rosmarinic acid on HMGB1‐induced inflammatory responses in vitro and in vivo

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