Antioxidant activities of bambara groundnut (Vigna subterranea) protein hydrolysates and their membrane ultrafiltration fractions

Arise, Abimbola K.; Alashi, Adeola M.; Nwachukwu, Ifeanyi D.; Ijabadeniyi, Oluwatosin Ademola; Aluko, Rotimi E.; Amonsou, Eric O.

doi:10.1039/c6fo00057f

Cited by 91 publications

(62 citation statements)

References 24 publications

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“…The fraction containing 3-5 kDa MW peptides presented the highest antioxidant capacity, but without significantly (p > 0.05) differing from the fraction consisting of peptides with a MW < 3 kDa. These results contrast with the behavior shown by bambara groundnut Alcalase-protein hydrolysates, with the ferric reducing power been practically independent of MW [19].…”

Section: Antioxidant Properties Of Peptide Fractions Obtained By Ultrcontrasting

confidence: 96%

Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Hou

Wang

et al. 2019

Antioxidants

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In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

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Section: Antioxidant Properties Of Peptide Fractions Obtained By Ultrcontrasting

confidence: 96%

Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Hou

Wang

et al. 2019

Antioxidants

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“…Recently, many studies are interested in generating bioactive peptides from plant protein sources. Some factors that influence the bioactivity of peptide are size, amino acid composition and sequence (Arise et al, 2016). Generally, bioactive peptides have two to twenty amino acid residues per chain and often contain high proportion of hydrophobic amino acid.…”

Section: Introductionmentioning

confidence: 99%

Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates

Sonklin

Laohakunjit

Kerdchoechuen

2018

Preprint

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Background Bioactive peptides can prevent damage associated with oxidative stress in the human body when consumed regularly. Recently year, peptides have attracted immense interest because of their beneficial functional properties, safety and little or no side effects when used at high concentration. Most antioxidant peptide has small size less than 1 kDa and contains high proportion of hydrophobic amino acid. Particularly Tyr, Leu, Ala, Ile, Val, Lys, Phe, Cys, Met and His exhibited high antioxidant activity. Mungbean protein contain high abundance of protein and hydrophobic amino acid contents, investigating its bioactivity is an important aspect of adding value to this by-product obtained from a growing industry. Therefore, the objectives of this study were to optimize the conditions used to generate MMPH with antioxidant activity form bromelain and to investigate the antioxidant activities of each molecular weight peptide fraction. Methods Response Surface Methodology (RSM) was used for screening the optimal conditions to produce Mungbean meal protein hydrolysate (MMPH). After that optimal MMPH was fractionated using ultrafiltration membranes with different molecular weight (MW) distribution. Crude-MMPH and four peptide fractions were investigated for five antioxidant activities: DPPH scavenging activity, Hydroxyl scavenging activity, Superoxide scavenging activity, Ferric reducing antioxidant power and metal ion chelation activity. Results The optimal condition of crude-MMPH production was 12 % (w/w) of bromelain and hydrolysis time for 12 h. The EC50 of DPPH was the highest for the F4 peptide fraction (MW<1 kDa) at 0.5320 mg/mL. Metal ion chelating activity was generally weak, except for the F4 that had a value of 43.94% at a protein concentration of 5 mg/mL. The F4 also exhibited high hydroxyl and superoxide radical scavenging activities (54 and 65.1%), but poor activity for ferric reducing antioxidant power (0.102 mM Fe2+/mg protein) compared to other peptide fractions and crude-MMPH. Molecular weight and amino acid were the main factors that determined the antioxidant activities of these peptide fractions. Results show that F4 have high antioxidant potentials. Discussion The lowest MW Fraction (less than 1 kDa) showed the highest DPPH activity, superoxide-, hydroxyl-scavenging activity and metal chelation activity. On the other hand, this fraction had poor ferric reducing power. This showed that low molecular weight has an important effect on antioxidant activities. According to the mechanism of the reaction, the potential of antioxidant activity was divided into two main groups: hydrogen atom transfer (HAT) and single electron transfer (SET). Therefore, this finding suggests that the antioxidant mechanism of peptides obtained mungbean could react with many species of free radicals by multiple mechanisms. Mungbean meal peptide can be developed into multiple functional foods which possess both antioxidant properties and aroma/taste.

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“…). Similar findings have previously been reported for Mytilus Edulis (Park et al ., ), chicken skin (Onuh et al ., ) and bambara groundnut (Arise et al ., ) protein hydrolysates. However, results obtained from silver carp (Dong et al ., ) and poultry viscera (Jamdar et al ., ) protein hydrolysates are in contrast to the current observation.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activities of Rapana venosa meat and visceral mass during simulated gastrointestinal digestion and their membrane ultrafiltration fractions

Luo

Xing

Wang

et al. 2017

Int J of Food Sci Tech

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Summary Rapana venosa (Rv) is an abundant marine snail resource with high content of protein. The antioxidant activities of Rv meat and visceral mass during simulated gastrointestinal (GI) digestion and their membrane ultrafiltration fractions were evaluated. Results indicated that visceral mass possessed stronger antioxidant activities than meat. The simulated GI digestion increased the hydroxyl radical scavenging activity while decreased the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) scavenging activity and reducing power. After membrane ultrafiltration, there were three fractions, that is molecular weight (MW) > 10 kDa, MW 3.5–10 kDa and MW < 3.5 kDa. Fractions with MW > 10 kDa and MW < 3.5 kDa showed the highest hydroxyl, DPPH radical scavenging activity, respectively. Fractions with MW 3.5–10 kDa and MW > 10 kDa showed the highest reducing power for meat and visceral mass, respectively. Rv hydrolysates exhibited significantly higher hydroxyl radical scavenging activity than the positive control vitamin C (Vc) and may serve as useful ingredients for application in food industry nutritional products.

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Antioxidant activities of bambara groundnut (Vigna subterranea) protein hydrolysates and their membrane ultrafiltration fractions

Cited by 91 publications

References 24 publications

Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates

Antioxidant activities of Rapana venosa meat and visceral mass during simulated gastrointestinal digestion and their membrane ultrafiltration fractions

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